Validation and in vitro characterization of antibiotic-loaded bone cement release

Cabanillas, Paloma Frutos; Peña, Enma; Barrales‐Rienda, J. M.; Frutos, G.

doi:10.1016/s0378-5173(00)00520-2

Cited by 95 publications

(60 citation statements)

References 19 publications

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“…PMMA is a highly hydrophobic polymer, and is thus impervious to drug diffusion [31]. The release of ciprofloxacin from samples assayed can be explained by the Van De Belt theory [32], as during the first hours of the experiment (24 hours) only ciprofloxacin molecules located in the superficial layers were released.…”

Section: Discussionmentioning

confidence: 99%

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Study of the Influence of Bone Cement Type and Mixing Method on the Bioactivity and the Elution Kinetics of Ciprofloxacin

Martínez-Moreno

Mura

Merino

et al. 2015

The Journal of Arthroplasty

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Section: Discussionmentioning

confidence: 99%

“…Previous papers showed that around 3% of gentamicin [31,34] and vancomycin [18] were released. These values are very similar to the ones of our study, 2.5-5.5%, depending on the conditions.…”

Section: Discussionmentioning

confidence: 99%

Study of the Influence of Bone Cement Type and Mixing Method on the Bioactivity and the Elution Kinetics of Ciprofloxacin

Martínez-Moreno

Mura

Merino

et al. 2015

The Journal of Arthroplasty

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“…10,20 Three discs of different diameter NT-G were each individually immersed in 1 mL of PBS in a 24-well plate at a temperature of 37°C with an agitation at 100 rpm. FlatTi in 1 mL of PBS was used as a parallel control.…”

Section: Characterization Of Drug Release From Tntsmentioning

confidence: 99%

“…The samples were analyzed for drug content using a colorimetric assay. 20 Briefly, the o-phthaldialdehyde reagent was formulated by adding 2.5 g of o-phthaldialdehyde (Sigma-Aldrich), 62.5 mL of methanol (Sigma-Aldrich), and 3 mL of 2-mercaptoethanol (Sigma-Aldrich) to 560 mL of sodium borate (Sigma-Aldrich) in distilled water. The reagent was stored in a brown bottle in a dark chamber for at least 24 hours prior to use.…”

Section: Characterization Of Drug Release From Tntsmentioning

confidence: 99%

Inhibited bacterial biofilm formation and improved osteogenic activity on gentamicin-loaded titania nanotubes with various diameters

Lin¹,

Tan²,

Duan³

et al. 2014

IJN

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Titania nanotubes loaded with antibiotics can deliver a high concentration of antibiotics locally at a specific site, thereby providing a promising strategy to prevent implant-associated infections. In this study we have fabricated titania nanotubes with various diameters (80, 120, 160, and 200 nm) and 200 nm length via electrochemical anodization. These nanotubes were loaded with 2 mg of gentamicin using a lyophilization method and vacuum drying. A standard strain, Staphylococcus epidermidis (American Type Culture Collection 35984), and two clinical isolates, S. aureus 376 and S. epidermidis 389, were selected to investigate the anti-infective ability of the gentamicin-loaded nanotubes (NT-G). Flat titanium (FlatTi) and nanotubes with no drug loading (NT) were also investigated and compared. We found that NT-G could significantly inhibit bacterial adhesion and biofilm formation compared to FlatTi or NT, and the NT-G with 160 nm and 200 nm diameters had stronger antibacterial activity because of the extended drug release time of NT-G with larger diameters. The NT also exhibited greater antibacterial ability than the FlatTi, while nanotubes with 80 nm or 120 nm diameters had better effects. Furthermore, human marrow derived mesenchymal stem cells were used to evaluate the effect of nanotubular topographies on the osteogenic differentiation of mesenchymal stem cells. Our results showed that NT-G and NT, especially those with 80 nm diameters, significantly promoted cell attachment, proliferation, spreading, and osteogenic differentiation when compared to FlatTi, and there was no significant difference between NT-G and NT with the same diameter. Therefore, nanotube modification and gentamicin loading can significantly improve the antibacterial ability and osteogenic activity of orthopedic implants.

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“…11 Further details on this method can be found elsewhere. 11,24 For the spectrophotometric determination, equal volumes of GTMcontaining solutions, o-Phtaldialdehyde reagent and isopropanol were mixed. After 30 min of incubation at room temperature, the absorbance was measured at 332 nm.…”

mentioning

confidence: 99%

Presence of starch enhances in vitro biodegradation and biocompatibility of a gentamicin delivery formulation

et al. 2014

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The effect of α-amylase degradation on the release of gentamicin from starch-conjugated chitosan microparticles was investigated up to 60 days. Scanning electron microscopic observations showed an increase in the porosity and surface roughness of the microparticles as well as reduced diameters. This was confirmed by 67% weight loss of the microparticles in the presence of α-amylase. Over time, a highly porous matrix was obtained leading to increased permeability and increased water uptake with possible diffusion of gentamicin. Indeed, a faster release of gentamicin was observed with α-amylase. Starch-conjugated chitosan particles are non-toxic and highly biocompatible for an osteoblast (SaOs-2) and fibroblast (L929) cell line as well as adipose-derived stem cells. When differently produced starch-conjugated chitosan particles were tested, their cytotoxic effect on SaOs-2 cells was found to be dependent on the crosslinking agent and on the amount of starch used.

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Validation and in vitro characterization of antibiotic-loaded bone cement release

Cited by 95 publications

References 19 publications

Study of the Influence of Bone Cement Type and Mixing Method on the Bioactivity and the Elution Kinetics of Ciprofloxacin

Study of the Influence of Bone Cement Type and Mixing Method on the Bioactivity and the Elution Kinetics of Ciprofloxacin

Inhibited bacterial biofilm formation and improved osteogenic activity on gentamicin-loaded titania nanotubes with various diameters

Presence of starch enhances in vitro biodegradation and biocompatibility of a gentamicin delivery formulation

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