Validation and implementation of an internal standard in comet assay analysis

Boeck, Marlies De; Touil, Nadia; Visscher, Geofrey De; Vande, Peter Aka; Kirsch‐Volders, Micheline

doi:10.1016/s1383-5718(00)00075-9

Cited by 114 publications

(80 citation statements)

References 12 publications

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“…33,34) The comet tail moment, tail DNA%, and tail length are the most frequently used descriptors to predict DNA damage. 18,35,36) LPS resulted in a significant increase in tail moment, tail DNA%, and tail length and there was a significant positive correlation of tail length with tail moment (r=0.884, p<0.001) and olive moment (r=0.908, p<0.001), indicating that LPS is genotoxic. LPS at 1 mg/kg/bw intraperitonally induces 33% increase in damage of DNA lymphocyte accompanied by a higher tail length of 6.911 µm in male rats.…”

Section: ±06mentioning

confidence: 94%

Indoxacarb interaction alters immunotoxic and genotoxic potential of endotoxin

Kaur

Mukhopadhyay

Arora

et al. 2016

Journal of Pesticide Science

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Indoxacarb is commonly used to effectively control pests, cockroaches, termites, fleas, and houseflies. Although the toxicological profile of indoxacarb had already been well characterized, we examined the possible toxicological interaction with indoxacarb and endotoxin. Male Swiss albino mice aged 8-10 weeks were orally administered indoxacarb dissolved in groundnut oil at 4 mg/ kg/day and 2 mg/kg/day for 90 days. On day 91, five animals from each group were challenged with lipopolysaccharides (LPS) at 80 µg/mouse, administered intranasally. Indoxacarb at 4 mg/kg significantly decreased Total leukocyte count, lymphocytopenia, and neutrophilia. Both doses of indoxacarb combined with LPS resulted in significant lymphocytopenia. Indoxacarb did not produce DNA damage in comet assay, but when combined with LPS, it resulted in a significant increase in tail length, tail moment, and olive moment. The data indicate that indoxacarb at 4 mg/kg administered orally for 90 days induced immune-response change. Further, both doses of indoxacarb, when combined with LPS, accelerate immunotoxicity and endotoxin-induced DNA damage.

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Section: ±06mentioning

confidence: 94%

Indoxacarb interaction alters immunotoxic and genotoxic potential of endotoxin

Kaur

Mukhopadhyay

Arora

et al. 2016

Journal of Pesticide Science

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“…Several arguments are in favor of the use of % TD. De Boeck et al (2000) demonstrated less inter-electrophoresis and inter-experimenter variability when using % TD compared to TL (De Boeck et al 2000). Collins (2004) argues that TL can be useful at low DNA damage levels, but not at higher levels of DNA damage and that TL is more sensitive to background and threshold settings of the image analysis (Collins, 2004).…”

Section: Alkaline Comet Assaymentioning

confidence: 99%

Genomic Integrity of Mouse Embryonic Stem Cells

Leyns¹,

Gonzalez²

2012

Embryogenesis

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“…Tail moment (TM) was measured by the computerized image analysis system and encompasses both the length of DNA migration in the comet tail and the tail intensity. TM is considered to be one of the best indices of induced DNA damage among the various parameters (De Boeck et al, 2000).…”

Section: Animal Treatment and Genetic Diagnosismentioning

confidence: 99%

“…The consequences of exposure and metabolism of carcinogens can be assessed by DNA damage as revealed by the level of DNA bulky adducts or by the number of DSBs (Farmer et al, 2003;Husgafvel-Pursiainen, 2004). Also, DSBs may result from the incomplete excision repair of DNA adducts, cross-links and alkali-labile sites when high alkaline conditions occur (Pfuhler and Wolf, 1996;De Boeck et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

DNA damage and repair process in earthworm after in-vivo and in vitro exposure to soils irrigated by wastewaters

Qiao

Chen

Wang

et al. 2007

Environmental Pollution

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A non-linear dose-response relationship exists between earthworm DNA damage, measured with comet assay, and total PAHs levels in soils irrigated by wastewaters. AbstractIn this study, DNA damage to earthworms (Eisenia fetida) after in vivo exposure to contaminated soils was measured by detecting DNA strand breakages (DSBs) and causality was analyzed through fractionation based bioassays. A non-linear dose-response relationship existed between DNA damage and total soil PAHs levels. DNA damage, measured with the comet assay, and its repair process, were observed. To identify the chemical causality, an in vitro comet assay using coelomocytes was subsequently performed on the fractionated organic extracts from soils. The results showed that the PAHs in the soils were responsible for the exerting genotoxic effects on earthworms. When normalized to benzo(a)pyrene toxic equivalent (TEQ BaP ), the saturation dose in the dose-response curve was about 10 ng TEQ BaP g À1 soil (dw).

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Validation and implementation of an internal standard in comet assay analysis

Cited by 114 publications

References 12 publications

Indoxacarb interaction alters immunotoxic and genotoxic potential of endotoxin

Indoxacarb interaction alters immunotoxic and genotoxic potential of endotoxin

Genomic Integrity of Mouse Embryonic Stem Cells

DNA damage and repair process in earthworm after in-vivo and in vitro exposure to soils irrigated by wastewaters

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