Validation and application of a method for the simultaneous absolute quantification of 16 neutral and acidic human milk oligosaccharides by graphitized carbon liquid chromatography – electrospray ionization – mass spectrometry

Tonon, Karina M.; Miranda, Antônio; Abrão, Ana Cristina Freitas de Vilhena; Morais, Mauro Batista de; Morais, Tania Beninga

doi:10.1016/j.foodchem.2018.09.036

Cited by 58 publications

(42 citation statements)

References 36 publications

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“…HMOs were extracted from human milk, identified and quantified by liquid chromatography-mass spectrometry (LC-MS) following a validated method previously reported in detail [41]. Analyses were performed in duplicate for each human milk sample and HMOs concentrations were reported as the mean value of the duplicates.…”

Section: Methodsmentioning

confidence: 99%

Maternal and Infant Factors Associated with Human Milk Oligosaccharides Concentrations According to Secretor and Lewis Phenotypes

et al. 2019

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Human milk oligosaccharides (HMOs) are multifunctional carbohydrates naturally present in human milk that act as prebiotics, prevent pathogen binding and infections, modulate the immune system and may support brain development in infants. HMOs composition is very individualized and differences in HMOs concentrations may affect the infant’s health. HMOs variability can be partially explained by the activity of Secretor (Se) and Lewis (Le) genes in the mother, but non-genetic maternal factors may also be involved. In this cross-sectional, observational study, 78 single human milk samples ranging from 17 to 76 days postpartum (median: 32 days, IQR: 25–46 days) were collected from breastfeeding Brazilian women, analyzed for 16 representative HMOs by liquid chromatography coupled to mass spectrometry and associations between maternal and infant factors with HMOs concentrations were investigated. HMOs concentrations presented a high variability even in women with the same SeLe phenotype and associations with maternal allergic disease, time postpartum and with infant’s weight, weight gain and sex. Overall, we present unprecedented data on HMOs concentrations from breastfeeding Brazilian women and novel associations of maternal allergic disease and infant’s sex with HMOs concentrations. Differences in HMOs composition attributed to maternal SeLe phenotype do not impact infant growth, but higher concentrations of specific HMOs may protect against excessive weight gain.

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Section: Methodsmentioning

confidence: 99%

Maternal and Infant Factors Associated with Human Milk Oligosaccharides Concentrations According to Secretor and Lewis Phenotypes

et al. 2019

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“…A large amount of studies on hMOS start with removing or reducing the lipid content of the milk by centrifugation [44,57,73,81,107,108,114,115,116,117]. In some studies, the fat layer on top of the sample is scooped off the milk, in other studies the clear liquid is pipetted into a new vial, carefully avoiding transferral of fat to the new vial.…”

Section: Analysis Of Hmosmentioning

confidence: 99%

Challenges and Pitfalls in Human Milk Oligosaccharide Analysis

Leeuwen

2019

Nutrients

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Human milk oligosaccharides have been recognized as an important, functional biomolecule in mothers’ milk. Moreover, these oligosaccharides have been recognized as the third most abundant component of human milk, ranging from 10–15 g/L in mature milk and up to and over 20 g/L reported in colostrum. Initially, health benefits of human milk oligosaccharides were assigned via observational studies on the differences between breastfed and bottle fed infants. Later, pools of milk oligosaccharides were isolated and used in functional studies and in recent years more specific studies into structure–function relationships have identified some advanced roles for milk oligosaccharides in the healthy development of infants. In other research, the levels, diversity, and complexity of human milk oligosaccharides have been studied, showing a wide variation in results. This review gives a critical overview of challenges in the analysis of human milk oligosaccharides. In view of the myriad functions that can be assigned, often to specific structures or classes of structures, it is very relevant to assess the levels of these structures in the human milk correctly, as well as in other biological sample materials. Ultimately, the review makes a case for a comparative, inter-laboratory study on quantitative human milk oligosaccharide analysis in all relevant biological samples.

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“…The deprotonated ion peaks [M-H] − , as well as the fragmentation patterns of the detected oligosaccharides had not previously identified from any natural resources (Fukuda et al, 2010;Elkhayat et al, 2012;Sandjo and Kuete, 2013;Alhaj et al, 2013;Albrecht et al, 2014;Urashima et al, 2016;Chen et al, 2018;Gangwar et al, 2018;Robinson et al, 2018;Tonon et al, 2019).…”

Section: Identification Of Oligosaccharidesmentioning

confidence: 99%

“…Free oligosaccharides are the most abundant solid constituent of milk, apart from lactose and lipids. Several oligosaccharides have been identified in milk from various sources, containing different structural moieties of sugars: lactose, glucose, N-acetyl glucosamine galactose, N-acetylgalactosamine, mannose, and fucose (Singh et al, 2016;Srivastava et al, 2016;Urashima et al, 2016;Gangwar et al, 2017;Robinson et al, 2018;Urashima et al, 2018;Tonon et al, 2019). Recently, a new oligosaccharide has been isolated from CM and named medalose (Gangwar et al, 2018).…”

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confidence: 99%

Electrospray ionization mass spectrometry characterization of ubiquitous minor lipids and oligosaccharides in milk of the camel (Camelus dromedarius) and their inhibition of oxidative stress in human plasma

Hamed

Said

Kontek

et al. 2020

Journal of Dairy Science

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The aim of this study was to characterize minor lipids in methanol fraction extracted from raw camel milk after loading it on a water-preconditioned short C18 open column and fractionating with a gradient of methanol/water. The C18 column showed high fractionation efficiency of minor lipids, such as glycosphingolipids, lipopolysaccharides, or oligosaccharides, when compared with other constituents, in particular polysaccharides, proteins, and free fatty acids. Liquid chromatography electrospray ionization tandem mass spectrometry in negative ion mode was used to identify 21 new glycosphingolipids, lipopolysaccharides, and oligosaccharides. Electrospray ionization tandem mass spectrometry was qualified to provide relevant data for recognizing the molecular mass, glycosylation sequences, and structure of saccharide moieties for the revealed compounds. The sequence of combinations of one selected lipopolysaccharide, which was considered the backbone of the remaining lipopolysaccharides, was confirmed in a density functional theory study. The obtained results showed that the tested fraction is a rich source of glycosphingolipids, lipopolysaccharides, and oligosaccharides with antioxidant activity.

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Validation and application of a method for the simultaneous absolute quantification of 16 neutral and acidic human milk oligosaccharides by graphitized carbon liquid chromatography – electrospray ionization – mass spectrometry

Cited by 58 publications

References 36 publications

Maternal and Infant Factors Associated with Human Milk Oligosaccharides Concentrations According to Secretor and Lewis Phenotypes

Maternal and Infant Factors Associated with Human Milk Oligosaccharides Concentrations According to Secretor and Lewis Phenotypes

Challenges and Pitfalls in Human Milk Oligosaccharide Analysis

Electrospray ionization mass spectrometry characterization of ubiquitous minor lipids and oligosaccharides in milk of the camel (Camelus dromedarius) and their inhibition of oxidative stress in human plasma

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