Validated voltammetric determination of olmesartan medoxomil: Method development and electrochemical behaviors investigation

Çelebi̇er, Mustafa; Süslü, İncilay; Altınöz, Sacide

doi:10.1039/c2ay26138c

Cited by 5 publications

(4 citation statements)

References 24 publications

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“…The plasma recovery ( Table 2 ) was found to be more than 85% for both of the drugs at different concentration levels suitable for bioanalytical analysis. Furthermore, the specificity and matrix effect of the formulation component were evaluated at three dose levels (1, 2, and 4 μ g/ml), employing a standard addition method [ 22 ]. The developed method is found to be specific for the coestimation of the PTX and DOX in the developed formulation system with matrix effect ≤ ±2% ( Figure 4(c) ).…”

Section: Resultsmentioning

confidence: 99%

“…The developed formulation was lyophilized and further analyzed for specificity, matrix effect, entrapment, and loading efficiency utilizing the developed HPLC method [ 21 ]. To evaluate the matrix effect and specificity of the developed method, blank formulation was fortified with known amount of drug and dissolved in methanol to disrupt the nanocarrier [ 22 ]. The samples were then analyzed by the developed HPLC method for PTX and DOX in the presence of formulation components.…”

Section: Methodsmentioning

confidence: 99%

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Validated HPLC-UV Method for Simultaneous Estimation of Paclitaxel and Doxorubicin Employing Ion Pair Chromatography: Application in Formulation Development and Pharmacokinetic Studies

Saklani

Tiwari

Yadav

et al. 2022

BioMed Research International

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Though paclitaxel (PTX) and doxorubicin (DOX) are amongst the most widely used and investigated drug pair for combination chemotherapy but surprisingly, not a single validated HPLC-UV method is available to analyze PTX and DOX simultaneously. So, herein a HPLC-UV method is developed and validated for the same, filling an indispensable gap in the literature. As these two moieties have characteristically different polarities, resolving them under the common chromatographic conditions is a challenging task. Herein, the principle of ion pair chromatography is utilized to resolve these two moieties on a C18 column employing an isocratic mobile phase comprised of acetonitrile and octane sulfonic acid buffer (67 : 37) and detected simultaneously at 231 nm using a UV detector only. The retention time is 4.4 and 7.2 min for PTX and DOX, respectively, with a total analysis time of less than 10 minutes, suitable for the formulation development and research, while LOQ is less than 0.066 μg/ml for both the drugs, suitable for the therapeutic drug monitoring at preclinical and clinical research setup. To substantiate the applicability of the developed method, a nanoformulation coloaded with PTX and DOX was designed and analyzed using the developed protocol. The method is also applied successfully to study the plasma kinetic profile of both the moieties simultaneously in Balb/c mice. Further, the method is validated as per the ICH guidelines fulfilling the unmet need of a validated analytical tool to simultaneously estimate PTX and DOX. Moreover, the results suggest that the principal of common ion chromatography demonstrated here can also be applied further for the simultaneous chromatographic separation of other polar and nonpolar moieties too. Consequently, the reported method surely will advance the toolset required for the precision-based combination chemotherapy.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Validated HPLC-UV Method for Simultaneous Estimation of Paclitaxel and Doxorubicin Employing Ion Pair Chromatography: Application in Formulation Development and Pharmacokinetic Studies

Saklani

Tiwari

Yadav

et al. 2022

BioMed Research International

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“…However, according to the literature, the reduction behavior of OM at PtIr-PEDOT/CFP can be attributed to saturation of the azomethine group C=N in tetrazolyl moiety of OM. 14 The plausible mechanism has been schematically represented in Figure 14.…”

Section: X-ray Photoelectron Spectroscopy (Xps)-mentioning

confidence: 99%

“…Electroanalytical methods offer advantages over other analytical methods in terms of simplicity, cost, rapid detection, requirement of sample in small quantity, non-requirement of derivatizations and less sensitivity toward matrix effects. [12][13][14][15] Surprisingly, there are only three reports based on electroanalytical methods for OM determination in bulk and dosage forms. These methods suffer from disadvantages like high detection limits, narrow linear dynamic range and complicated electrode preparation procedures.…”

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confidence: 99%

PEDOT Decorated with PtIr Nanoclusters on Carbon Fiber Paper toward Electrocatalytic Reduction of a Hypertensive Drug Olmesartan Medoxomil

Akshaya

Vinod

Nidhin

et al. 2018

J. Electrochem. Soc.

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A facile electrochemical sensor was developed by electrochemical deposition of PtIr nanoparticles on poly(3,4ethylenedioxythiophene) (PEDOT) modified carbon fiber paper electrode (CFP). The modified electrodes were characterized by Field emission scanning electron microscopy (FESEM) with energy-dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD), RAMAN spectroscopy, Fourier transform infrared spectroscopy (FTIR) and electroanalytical techniques. PtIr-PEDOT modified CFP electrode was used successfully in the determination of an anti-hypertensive prodrug Olmesartan medoxomil (OM) at femtomolar (fM) level for the first time. Under optimal conditions, the prepared electrochemical sensor has shown broad linear range between 0.001 pM and 0.220 pM and superior detection limit of 3.42 fM was observed based on Differential pulse voltammetric (DPV) study. In addition, the designed electrochemical sensor was successfully demonstrated as a novel platform in the electrochemical determination of OM in human urine and pharmaceutical samples.

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Applications for Drug Assays

Özkan

Kauffmann

Zuman

2015

Monographs in Electrochemistry

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Validated voltammetric determination of olmesartan medoxomil: Method development and electrochemical behaviors investigation

Cited by 5 publications

References 24 publications

Validated HPLC-UV Method for Simultaneous Estimation of Paclitaxel and Doxorubicin Employing Ion Pair Chromatography: Application in Formulation Development and Pharmacokinetic Studies

Validated HPLC-UV Method for Simultaneous Estimation of Paclitaxel and Doxorubicin Employing Ion Pair Chromatography: Application in Formulation Development and Pharmacokinetic Studies

PEDOT Decorated with PtIr Nanoclusters on Carbon Fiber Paper toward Electrocatalytic Reduction of a Hypertensive Drug Olmesartan Medoxomil

Applications for Drug Assays

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