Validated spectrofluorometric method for determination of gemfibrozil in self nanoemulsifying drug delivery systems (SNEDDS)

Abstract

HIGHLIGHTSA spectrofluorometric method has been developed for the determination of gemfibrozil.The method is based on the excitation and emission capacities of gemfibrozil.This method allows de determination of the drug in SNEDDS.Results showed linear relationships, low limits of LOD and LOQ with good robustness. GRAPHICAL ABSTRACTValidated spectofluorometric method for determination of gemfibrozil in self nanoemulsifying drug delivery systems (SNEDDS) Article history: ABSTRACT A spectrofluorometric method has been developed and validated for the determination of gemfibrozil. The method is based on the excitation and emission capacities of gemfibrozil with excitation and emission wavelengths of 276 and 304 nm respectively. This method allows de determination of the drug in a selfnanoemulsifying drug delivery system (SNEDDS) for improve its intestinal absorption. Results obtained showed linear relationships with good correlation coefficients (r 2 > 0.999) and low limits of detection and quantification (LOD of 0.075 μg mL −1 and LOQ of 0.226 μg mL −1 ) in the range of 0.2-5 μg mL −1 , equally this method showed a good robustness and stability. Thus the amounts of gemfibrozil released from SNEDDS contained in gastro resistant hard gelatine capsules were analysed, and release studies could be performed satisfactorily.

Smart Nanoscale Extracellular Vesicles in the Brain: Unveiling their Biology, Diagnostic Potential, and Therapeutic Applications

“…In this context, Hao et al specifically generated microglia targeting exosomes loaded with gemfibrozil (MExo-Gem), a drug for hyperlipidemia. 110 They showed that when injected in mice, these exosomes could facilitate phagocytic internalization of Aβ aggregates in the microglia. This effect resulted in improved cognition in the AD mice.…”

“…Other than tissue-derived EVs, EVs are also engineered in vitro for better therapeutic performance. In this context, Hao et al specifically generated microglia targeting exosomes loaded with gemfibrozil (MExo-Gem), a drug for hyperlipidemia . They showed that when injected in mice, these exosomes could facilitate phagocytic internalization of Aβ aggregates in the microglia.…”

Magnetic solid phase extraction of gemfibrozil from human serum and pharmaceutical wastewater samples utilizing a β-cyclodextrin grafted graphene oxide-magnetite nano-hybrid

“…Gemfibrozil, 5-(2,5-dimethylphenoxy)-2,2-dimethylpentanoic acid, is a benzene derivative of valeric acid belonging to a drug group known as fibrates [17]. It is clinically effective in reducing serum cholesterol and triglyceride levels.…”

“…It has also been demonstrated that this drug lowers the incidence of coronary heart disease in humans [18]. Several methods have been developed for the determination of gemfibrozil in biological samples, environmental substances and pharmaceutical formulations, including high performance liquid chromatography with fluorescence detection, liquid chromatography-tandem mass spectrometry, gas chromatography-mass spectrometry and spectrofluorometry [17][18][19][20][21][22].…”

Construction and characterization of GCE/MWCNT/Au-NP as a new impedimetric and voltammetric sensor for determination of gemfibrozil in pharmaceutical and biological samples

“…Thus an accurate and sensitive quantification of GEM in pharmaceutical formulations and biological fluids such as blood plasma is required for quality-control procedures in the pharmaceutical industry and physiological pharmaco-kinetics studies. Reviewing the articles published in journals shows that different methods such as high performance liquid chromatography [4][5][6], gas chromatography [7,8], spectrofluorimetry [9][10][11], thin-layer chromatography [12], and polarography [13] have been reported for GEM measurement in clinical and pharmaceutical samples. Although these methods have good sensitivities in the determination of GEM, they suffer from limitations such as expensive equipment, operation complexity, and high consumption of reagents.…”