Validated Spectrofluorimetric Method For The Determination of Cefoxitin Sodium in Its Pure Form and Powder for Injection via Derivatization with 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl)

Abstract

Objectives: An accurate and precise spectrofluorimetric methodwas developed and validated for the determination of β-lactam antibiotic named; cefoxitin sodiumin its pure form and powder for injection. Methods: Based on nucleophilic substitution reaction of target drug with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) to form a highly fluorescent fluorophore measured at 540 nm after excitation at 460 nm. Results: Under optimum condition, the proposed method obeys Beer's law in range (0.5-7 µg mL -1 ) and the reaction mechanisms were presented. Conclusion: The method was validated according to ICH guideline for accuracy, precision and was successfully applied for the determination of the drug in its pure form and powder for injection. The obtained results were statistically compared with those of the reported method and found to be in good agreement.

Eco-friendly analytical methods for the determination of compounds with disparate spectral overlapping: application to antiviral formulation of sofosbuvir and velpatasvir

“…6c, d). This decrease in fluorescence was previously reported (El-Yazbi et al, 2016;Darwish et al, 2009;Attia et al, 2017) and was attributed to the formation of NBD-OH in the excess quantity of hydroxide ion that prohibits the reaction of NBD-Cl with the drug. Phosphate buffer with the same pH was also tested and compared with 0.2 M borate buffer.…”

“…In the NBD-Cl method, VP was found to react with NBD-Cl in borate buffer pH 8 yielding a highly fluorescent yellow product with maximum absorption at 436 nm and exhibiting strong fluorescence at 550 nm, after excitation at 480 nm (Fig. 5b) (Miyano et al, 1985;Walash et al, 2011;El-Yazbi et al, 2016;Darwish et al, 2009;Attia et al, 2017;Annenkov et al, 2015;Rageh et al, 2010;Omar et al, 2017;Mohamed et al, 2019). This reaction occurs as NBD-Cl reacts with the amine group in the VP under mild alkaline conditions yielding a highly fluorescent yellow colored derivative as shown in Scheme 1 (Miyano et al, 1985;Walash et al, 2011;El-Yazbi et al, 2016;Darwish et al, 2009;Attia et al, 2017;Annenkov et al, 2015;Rageh et al, 2010;Omar et al, 2017;Mohamed et al, 2019).…”