Validated liquid chromatographic-fluorescence method for the quantitation of gemifloxacin in human plasma

Al-Hadiya, Badraddin M.H.; Khady, Adnan A.; Mostafa, Gamal A. E.

doi:10.1016/j.talanta.2010.08.047

Cited by 35 publications

(29 citation statements)

References 13 publications

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“…Linear regression equation and correlation coefficient (R 2 ) were employed to statistically evaluate the linearity of the results (Ali et al, 2006;Lister, 2005;Al-Hadiya et al, 2010).…”

Section: Linearity and Rangementioning

confidence: 99%

“…To evaluate the specificity of the method, drug free lipid formulation (QC zero ) samples were carried out through the assay procedure and the retention times of the lipid formulation components were compared with those of ACV analyte (Al-Hadiya et al, 2010). In addition, specificity of the method toward the intact drug was also studied by determination of the resolution (R) between the drug peak and the nearest degradation product if present (Dongre et al, 2008).…”

Section: Specificitymentioning

confidence: 99%

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Development and validation of a UPLC method for quantification of antiviral agent, Acyclovir in lipid-based formulations

Alamri

Kazi

Alanazi

2019

Arabian Journal of Chemistry

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Purpose: The objective of the current study is to evaluate the Ultra Performance Liquid Chromatography (UPLC) method for quantification of Acyclovir in lipid-based formulations.Method: A simple, rapid, reliable and precise reversed phase UPLC method has been developed and validated according to the regulatory guidelines, which composed of isocratic mobile phase; 0.25% formic acid (FA) in Milli-Q water with a flow rate of 0.5 ml/min, and column BEH C18 (2.1 · 50 mm, 1.7 lm). The detection was carried out at 254 nm.Results: The developed UPLC method was found to be rapid (1.2 min run time), selective with well resoluted Acyclovir peak (0.89 min) from different lipid matrices and sensitive (Limit of Detection (LOD) was 0.3 ppm and Lower Limit of Quantification (LLOQ) was 1 ppm). The accuracy and precision were determined and were perfectly matching with the standard FDA limits.Conclusion: The study showed that the proposed UPLC method can be used for the assessment of drug purity, stability, solubility and lipid-formulation release profile with no interference of excipients or related substances of active pharmaceutical ingredient. ª 2014 Production and hosting by Elsevier B.V. on behalf of King Saud University.

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“…Linear regression equation and correlation coefficient (R 2 ) were employed to statistically evaluate the linearity of the results (Ali et al, 2006;Lister, 2005;Al-Hadiya et al, 2010).…”

Section: Linearity and Rangementioning

confidence: 99%

Section: Specificitymentioning

confidence: 99%

Development and validation of a UPLC method for quantification of antiviral agent, Acyclovir in lipid-based formulations

Alamri

Kazi

Alanazi

2019

Arabian Journal of Chemistry

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“…1). Few analytical methods appeared in the literature for the determination of this drug including spectrophotometric [21][22][23][24], LC [25][26][27], HPLC [28,29], electrophoresis [30,31] and spectrofluorimetry [32,33] were reported for analysis of GFX.…”

Section: Introductionmentioning

confidence: 99%

Supramolecular interaction of gemifloxacin and hydroxyl propyl β-cyclodextrin spectroscopic characterization, molecular modeling and analytical application

Dsugi

Elbashir

Suliman

2015

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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“…Several methods have been reported for the determination of fluoroquinolones either in pure forms, in dosage forms, or in biological fluids like chromatography [3][4][5][6][7][8][9], capillary zone electrophoresis [10,11], electrochemistry [12][13][14][15], atomic absorption spectrometry [16,17], spectrofluorimetry [18][19][20][21] and spectrophotometric methods for GMF [22][23][24][25][26][27][28], MOX [16,[29][30][31][32][33] and GTF [16,[34][35][36][37][38][39][40][41][42][43][44]. These methods are associated with some major drawbacks such as decreased selectivity due to measurement in ultraviolet region [22,23,29,30] and/or de...…”

Section: Introductionmentioning

confidence: 99%

Kinetic spectrophotometric determination of some fluoroquinolone antibiotics in bulk and pharmaceutical preparations

Wahed¹,

Sheikh²,

Gouda³

et al. 2013

Bull. Chem. Soc. Eth.

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ABSTRACT. A simple and sensitive kinetic spectrophotometric method was developed for the determination of some fluoroquinolonea antibiotics; gemifloxacin mesylate, moxifloxacin hydrochloride and gatifloxacin in bulk and in pharmaceutical preparations. The method is based upon a kinetic investigation of the oxidation reaction of the drugs with alkaline potassium permanganate at room temperature for a fixed time of 20 min for gemifloxacin and 15 min for moxifloxacin or gatifloxacin. The absorbance of the coloured manganate ion was measured at 610 nm. The absorbance-concentration plots were rectilinear over the ranges of 2.0-20, 4.0-24 and 4.0-40 µg mL −1 for gemifloxacin, moxifloxacin and gatifloxacin, respectively. The concentrations of the studied drugs were calculated using the corresponding calibration equations for the fixed-time method. The determination of the studied drugs by the fixed concentration and rate constant methods was also feasible with the calibration equations obtained but the fixed time method has been found to be more applicable. The different experimental parameters affecting the development and stability of the colors were carefully studied and optimized. The proposed method was applied to the determination of the studied drugs in pharmaceutical formulations.

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Validated liquid chromatographic-fluorescence method for the quantitation of gemifloxacin in human plasma

Cited by 35 publications

References 13 publications

Development and validation of a UPLC method for quantification of antiviral agent, Acyclovir in lipid-based formulations

Development and validation of a UPLC method for quantification of antiviral agent, Acyclovir in lipid-based formulations

Supramolecular interaction of gemifloxacin and hydroxyl propyl β-cyclodextrin spectroscopic characterization, molecular modeling and analytical application

Kinetic spectrophotometric determination of some fluoroquinolone antibiotics in bulk and pharmaceutical preparations

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