Validated HPTLC method for assay of prednisolone in tablets and comparison with pharmacopeial methods

Mehta, Astha; Thaker, Anil

doi:10.1556/jpc.23.2010.3.8

Cited by 6 publications

(1 citation statement)

References 8 publications

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“…Figure 1 shows the chemical structures of both drugs. Both ESO and PRD have been determined previously in the literature, alone or in the presence of other drugs, in different matrices using different chromatographic methods, such as HPLC and TLC (Chitlange, Chaturvedi, & Wankhede, 2011; El‐Kimary & Ragab, 2015; Mabrouk et al, 2019; Mehta & Thaker, 2011; Musharraf et al, 2012; Pawar et al, 2014; Ragab & EL‐Kimary, 2017; Raut et al, 2014), and different spectroscopic methods, including spectrophotometry, spectrofluorimetry, and chemometry (Belal et al, 2014; Chitlange, Chaturvedi, Tawargeri, et al, 2011; Chitlange, Chaturvedi, & Wankhede, 2011; Mabrouk et al, 2019; Manoharan, 2019; Manu et al, 2012; Mohamed et al, 2006; Singh & Verma, 2007; Sversut et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

US FDA–validated TLC method with four greenness assessment evaluations for simultaneous determination of prednisolone and esomeprazole in spiked human plasma

Abdelhamid

Magdy

Anwar

et al. 2022

Biomedical Chromatography

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Recently, prednisolone has been used in treating many medical conditions, such as autoimmune diseases and cancer. It is also prescribed to mitigate the respiratory complications caused by COVID-19 infection. It can cause some health complications, such as GIT ulcers, so it should be co-administered with proton-pump inhibitors, such as esomeprazole, to prevent the risk of ulcers. This work aims to develop an ecofriendly and sensitive TLC method for simultaneous determination of esomeprazole and prednisolone in their binary mixtures and spiked human plasma.Separation was performed using a mixture of ethyl acetate, methanol, and ammonia (9.5:0.5:0.1, v/v/v) as an eluting system with UV scanning at 245 nm. Dapoxetine was used as an internal standard to correct the variation during sampling. The resulting R f values for plasma, esomeprazole, prednisolone, and dapoxetine were 0.03, 0.51, 0.72 and 0.85, respectively. Four greenness assessment tools-national environmental method index, eco-scale assessments, analytical greenness metric approach (AGREE), and green analytical procedure index (GAPI)-were used to evaluate the greenness characteristics of the proposed method to the environment, and the results were acceptable and satisfactory. Validation parameters were checked according to the US FDA guidelines to achieve the international requirements for bioanalytical method validation, and the results were within the accepted ranges.

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Section: Introductionmentioning

confidence: 99%

US FDA–validated TLC method with four greenness assessment evaluations for simultaneous determination of prednisolone and esomeprazole in spiked human plasma

Abdelhamid

Magdy

Anwar

et al. 2022

Biomedical Chromatography

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Simultaneous Determination of Prednisolone Acetate and Moxifloxacin Hydrochloride in Bulk and in Eye Drop Using Rp–hptlc

Raut

Shirkhedkar

Ugale

et al. 2013

Journal of Liquid Chromatography & Related Technologies

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Development of HPLC-UV Method for Simultaneous Determination of Corticosteroid Co-Administered Immune Therapy

Abdelhamid

Farid

Anwar

et al. 2023

Journal of Chromatographic Science

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Prednisolone (PDS) has recently been utilized to treat a variety of medical disorders, including autoimmune illnesses and cancer. It is also used to treat coronavirus disease 2019 infection-related respiratory problems. Because it may induce health problems including gastrointestinal lesions and ulceration, it has to be used alongside other drugs like esomeprazole (ESM), which acts as a proton pump antagonist to reduce the probability of ulceration. As a result, the goal of this research is to create an environmentally safe and sensitive high-performance liquid chromatography (HPLC) approach for determining PDS and ESM in their binary combination and spiked human plasma. C8 column (100 × 4.6 mm, 5 μm) and gradient mobile phase elution were used to separate the studied drugs with ultraviolet recognition at 290 nm. Caffeine was utilized as an internal standard to adjust the sample variance. Plasma, caffeine, ESM and PDS all had tR values of 1.4, 3.5, 6.3 and 7.3, respectively. The suggested method’s greenness features were evaluated using three greenness evaluation tools: green analytical procedure index, analytical greenness metric approach and analytical eco-scale, and the findings were approved and satisfied. Validation parameters were evaluated in accordance with US-FDA recommendations in order to meet the global desires for biological analysis technique, acceptable limits were obtained.

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Validated HPTLC method for assay of prednisolone in tablets and comparison with pharmacopeial methods

Cited by 6 publications

References 8 publications

US FDA–validated TLC method with four greenness assessment evaluations for simultaneous determination of prednisolone and esomeprazole in spiked human plasma

US FDA–validated TLC method with four greenness assessment evaluations for simultaneous determination of prednisolone and esomeprazole in spiked human plasma

Simultaneous Determination of Prednisolone Acetate and Moxifloxacin Hydrochloride in Bulk and in Eye Drop Using Rp–hptlc

Development of HPLC-UV Method for Simultaneous Determination of Corticosteroid Co-Administered Immune Therapy

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