Validated HPLC–MS–MS method for simultaneous determination of atorvastatin and 2-hydroxyatorvastatin in human plasma—pharmacokinetic study

Bořek-Dohalský, V.; Huclová, Jitka; Barrett, B.; Němec, B.; Ulč, Ivan; Jelı́nek, Ivan

doi:10.1007/s00216-006-0655-3

Cited by 66 publications

(45 citation statements)

References 10 publications

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“…One possibility is that AVA could act as an adjuvant therapy. Wong and Davis recently showed that AVA did not potentiate the in vitro activity of dihydroartemisinin (DHA) in the 3D7 strain of P. falciparum (13) and also posited that AVA had no clinical relevance because AVA IC 50 s were well above the plasma concentrations achievable in humans taking high-dose AVA (0.1 to 0.5 M) (2).…”

mentioning

confidence: 99%

Atorvastatin as a Potential Antimalarial Drug: In Vitro Synergy in Combinational Therapy with Dihydroartemisinin

Savini

Souraud

Briolant

et al. 2010

Antimicrob Agents Chemother

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mentioning

confidence: 99%

Atorvastatin as a Potential Antimalarial Drug: In Vitro Synergy in Combinational Therapy with Dihydroartemisinin

Savini

Souraud

Briolant

et al. 2010

Antimicrob Agents Chemother

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“…All of these methods consisted buffers combination as mobile phase. Several methods also reported for determination of Statin's [30][31][32][33][34].…”

Section: Figmentioning

confidence: 99%

Simultaneous Determination of Ceftriaxone Sodium and Statin Drugs in Pharmaceutical Formulations and Human Serum by Rp-HPLC

Sultana

Arayne

Shahzad

2010

J. Chil. Chem. Soc.

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An accurate, sensitive and least time consuming reverse phase high performance liquid chromatographic (RP-HPLC) method for the estimation of ceftriaxone in the presence of statin drugs in formulation and human serum has been developed and validated. Chromatographic separation was conducted on prepacked Purospher Star, C18 (5 mm, 250 x 4.6 mm) column at room temperature using methanol:water:acetonitrile (70:15:20 v/v/v) as a mobile phase, pH adjusted at 2.8 with ortho-phosphoric acid and at a flow rate of 1.0 mL/minute, while UV detection was performed at 240 nm. The results obtained showed a good agreement with the declared content. The method shows good linearity in the range of 2.5-25 µg/mL with a correlation coefficient 0.9966 -0.9998 (inter-and intra-day RSD < 2 %).The limit of detection and quantification for ceftriaxone and statins in pharmaceutical formulation and serum were in the range 0.124 -1.006 µg/mL. Analytical recovery was >98.72 %. The proposed method may be used for the quantitative analysis of commonly administered statin's i.e. simvastatin, rosuvastatin atorvastatin and pravastatin alone or in combination with ceftriaxone from raw materials, dosage formulations and in serum. The established HPLC method is rapid, accurate and selective, because of its sensitivity and reproducibility.

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“…Some of those methods include enzyme inhibition assay [9], radioimmunoassay [10], electrochemical method [11], high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection [12][13][14][15], liquid chromatography-mass spectrometry [16], microbore liquid chromatography/electrospray ionization-tandem mass spectrometry [17], and liquid chromatography-tandem mass spectrometry (LC-MS/MS) [18][19][20][21][22][23][24][25][26][27]. Immunoassay methods lack specificity and, in some cases, are subject to problems with cross-reactive interferences.…”

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confidence: 99%

LC—MS/MS method for quantification of atorvastatin, o-hydroxyatorvastatin, p-hydroxyatorvastatin, and atorvastatin lactone in rat plasma

Crevar-Sakač

Vujić

Vujčić

et al. 2016

Acta Chromatographica

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Summary.A simple and sensitive liquid chromatography-tandem mass spectrometry method was developed for the quantification of atorvastatin, ortho-hydroxyatorvastatin, para-hydroxyatorvastatin, and atorvastatin lactone in rat plasma. Solid-phase extraction was used for preparation of samples. Rosuvastatin was chosen as an internal standard. Chromatographic separation was achieved on ZORBAX Eclipse C 18 Analytical, 4.6 × 100 mm (3.5 μm) column with a gradient mobile phase composed of acetonitrile and 0.1% acetic acid, at a flow rate of 400 μL min −1 . The column was kept at constant temperature (25 °C), and autosampler tray temperature was set at 4 °C. The following selected reaction monitoring (SRM) transitions were selected: (m/z, Q1 → Q3, collision energy) atorvastatin (559.47 → 440.03, 22 eV), atorvastatin lactone (541.36 → 448.02, 19 eV), orthohydroxyatorvastatin (575.20 → 440.18, 20 eV), para-hydroxyatorvastatin (575.54 → 440.18, 20 eV), and rosuvastatin (482.25 with selected combination of two fragments 257.77, 31 eV, and 299.81, 35 eV) in positive ion mode. The method was validated over a concentration range of 0.5-20 ng mL −1 for ortho-hydroxyatorvastatin and para-hydroxyatorvastatin and 0.1-20 ng mL −1 for atorvastatin and atorvastatin lactone with excellent linearity (r 2 ≥ 0.99). This method demonstrated acceptable precision and accuracy at four quality control concentration levels. The detection limits were 0.1 and 0.13 ng mL −1 for orthohydroxyatorvastatin and para-hydroxyatorvastatin, respectively, and 0.05 ng mL −1 for atorvastatin and atorvastatin lactone. All analytes were found to be stable at examined conditions. Validated method was applied for determination of atorvastatin and its metabolites in plasma of experimental animals.

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Validated HPLC–MS–MS method for simultaneous determination of atorvastatin and 2-hydroxyatorvastatin in human plasma—pharmacokinetic study

Cited by 66 publications

References 10 publications

Atorvastatin as a Potential Antimalarial Drug: In Vitro Synergy in Combinational Therapy with Dihydroartemisinin

Atorvastatin as a Potential Antimalarial Drug: In Vitro Synergy in Combinational Therapy with Dihydroartemisinin

Simultaneous Determination of Ceftriaxone Sodium and Statin Drugs in Pharmaceutical Formulations and Human Serum by Rp-HPLC

LC—MS/MS method for quantification of atorvastatin, o-hydroxyatorvastatin, p-hydroxyatorvastatin, and atorvastatin lactone in rat plasma

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