Validated electrochemical immunosensor for ultra-sensitive procalcitonin detection: Carbon electrode modified with gold nanoparticles functionalized sulfur doped MXene as sensor platform and carboxylated graphitic carbon nitride as signal amplification

Medetalibeyoğlu, Hilal; Beytur, Murat; Akyıldırım, Onur; Atar, Necip; Yola, Mehmet Lütfi

doi:10.1016/j.snb.2020.128195

Cited by 85 publications

(45 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are two-type voltammetric immunosensors including sandwich type containing label and label-free. To obtain sensitive biosensor signals, the sandwich-type immunosensors are especially used and applied to the complex samples [18]. In the preparation of sandwich-type immunosensors, the primer (capture) antibody is firstly immobilized to any sensor platform such as electrochemical surface.…”

Section: Introductionmentioning

confidence: 99%

Sensitive sandwich-type voltammetric immunosensor for breast cancer biomarker HER2 detection based on gold nanoparticles decorated Cu-MOF and Cu2ZnSnS4 NPs/Pt/g-C3N4 composite

Yola

2021

Microchim Acta

Self Cite

View full text Add to dashboard Cite

A sandwich-type sensitive voltammetric immunosensor for breast cancer biomarker human epidermal growth factor receptor 2 (HER2) detection was prepared. The electrochemical immunosensor was developed based on gold nanoparticles decorated copper-organic framework (AuNPs/Cu-MOF) and quaternary chalcogenide with platinum-doped graphitic carbon nitride (g-C 3 N 4 ). Cu 2 ZnSnS 4 nanoparticle (CZTS NP) quaternary chalcogenide with platinum (Pt)-doped g-C 3 N 4 composite (Pt/g-C 3 N 4 ) was tagged as CZTS NPs/Pt/g-C 3 N 4 . AuNPs/Cu-MOF composite was successfully synthesized by amidation reaction between AuNPs functionalized with amino group and Cu-MOFs containing carboxylic acid. After the conjugations of primer HER2 antibody and antigen HER2 protein to AuNPs/Cu-MOF as sensor platform, CZTS NPs/Pt/g-C 3 N 4 composite was prepared by one-pot hydrothermal method. After immune reaction of 30 min, the prepared HER2 immunosensor was characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), x-ray diffraction (XRD) method, x-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). The developed immunosensor showed high sensitivity with a detection limit of 3.00 fg mL −1 . Additional properties of the voltammetric immunosensor are high selectivity, stability, reproducibility, and reusability.

show abstract

Section: Introductionmentioning

confidence: 99%

Sensitive sandwich-type voltammetric immunosensor for breast cancer biomarker HER2 detection based on gold nanoparticles decorated Cu-MOF and Cu2ZnSnS4 NPs/Pt/g-C3N4 composite

Yola

2021

Microchim Acta

Self Cite

View full text Add to dashboard Cite

show abstract

“…A new sensor was fabricated using glassy carbon electrode (GCE) modified with gold nanoparticles and delaminated sulfur doped MXene for PCT detection ( Medetalibeyoglu et al, 2020 ). To the best of our knowledge, this sensor is the best in terms of the obtained LOD value (2 fg/mL), which is far better than already discussed.…”

Section: Inflammatory Biomarkers and Their Detection Platformsmentioning

confidence: 99%

“… 3.3–125 μg/mL 1.95 μg/mL Giorgi-Coll et al (2019) Optical C.I. 1.25–9000 ng/mL 0.38 ng/mL Sene Ingridi de Souza et al (2020) Optical Reflectance 50–350 pg/mL 0.52 and 0.08 pg/mL Khan et al (2020) Procalcitonin Electro/optical EL 100 fg/mL to 50 ng/mL 41 fg/mL Yang et al (2019) Electrochemical Amperometry 0.01 pg/mL to 10 ng/mL 0.002 pg/mL Liu et al (2019) Electrochemical Impedimetry 0.01–10 ng/mL 0.10 ng/mL Tanak et al (2019) Electrochemical DPV 0.001–25 ng/mL 3 × 10 −4 ng/mL Gao et al (2020) Electrochemical Amperometry 10 pg/mL to 50 ng/mL 0.15 pg/mL Abbas and Soomro (2019) Electrochemical DPV 0.01–1.0 pg/mL 2 fg/mL Medetalibeyoglu et al (2020) Electrochemical Amperometry 500 fg/mL to 50 ng/mL 130 fg/mL Qu et al (2020) Electrochemical ...…”

Section: Inflammatory Biomarkers and Their Detection Platformsmentioning

confidence: 99%

Advancement in biosensors for inflammatory biomarkers of SARS-CoV-2 during 2019–2020

Garg

Sharma

Singh

2021

Biosensors and Bioelectronics

View full text Add to dashboard Cite

COVID-19 pandemic has affected everyone throughout the world and has resulted in the loss of lives of many souls. Due to the restless efforts of the researchers working hard day and night, some success has been gained for the detection of virus. As on date, the traditional polymerized chain reactions (PCR), lateral flow devices (LFID) and enzyme linked immunosorbent assays (ELISA) are being adapted for the detection of this deadly virus. However, a more exciting avenue is the detection of certain biomarkers associated with this viral infection which can be done by simply re-purposing our existing infrastructure. SARS-CoV-2 viral infection triggers various inflammatory, biochemical and hematological biomarkers. Because of the infection route that the virus follows, it causes significant inflammatory response. As a result, various inflammatory markers have been reported to be closely associated with this infection such as C-reactive proteins, interleukin-6, procalcitonin and ferritin. Sensing of these biomarkers can simultaneously help in understanding the illness level of the affected patient. Also, by monitoring these biomarkers, we can predict the viral infections in those patients who have low SARS-CoV-2 RNA and hence are missed by traditional tests. This can give more targets to the researchers and scientists, working in the area of drug development and provide better prognosis. In this review, we propose to highlight the conventional as well as the non-conventional methods for the detection of these inflammatory biomarkers which can act as a single platform of knowledge for the researchers and scientists working for the treatment of COVID-19.

show abstract

“…Among the multiple functions of carbon nanomaterials, amplification of the electrochemical signals provided by (bio)electrochemical devices has found extensive applications (see Table 2 ) [ 115 , 116 , 117 , 118 , 119 , 120 , 121 , 122 , 123 ]. To accomplish signal amplification two main ways, involving different mechanistic strategies, can be considered: (a) increasing the loading of electrochemically detectable species or the redox mediators or the catalysts, and (b) enhancing the electrochemical response as the result of an improved electron transfer.…”

Section: Multifunctional Carbon Nanomaterialsmentioning

confidence: 99%

“… Preparation of carboxylated c-g-C 3 N 4 and Ab 2 bioconjugate ( up ) and sandwich-type immunosensor ( down ). Reproduced from [ 121 ] with permission. …”

Section: Figurementioning

confidence: 99%

Multimodal/Multifunctional Nanomaterials in (Bio)electrochemistry: Now and in the Coming Decade

et al. 2020

View full text Add to dashboard Cite

Multifunctional nanomaterials, defined as those able to achieve a combined effect or more than one function through their multiple functionalization or combination with other materials, are gaining increasing attention in the last years in many relevant fields, including cargo targeted delivery, tissue engineering, in vitro and/or in vivo diseases imaging and therapy, as well as in the development of electrochemical (bio)sensors and (bio)sensing strategies with improved performance. This review article aims to provide an updated overview of the important advances and future opportunities exhibited by electrochemical biosensing in connection to multifunctional nanomaterials. Accordingly, representative aspects of recent approaches involving metal, carbon, and silica-based multifunctional nanomaterials are selected and critically discussed, as they are the most widely used multifunctional nanomaterials imparting unique capabilities in (bio)electroanalysis. A brief overview of the main remaining challenges and future perspectives in the field is also provided.

show abstract

Validated electrochemical immunosensor for ultra-sensitive procalcitonin detection: Carbon electrode modified with gold nanoparticles functionalized sulfur doped MXene as sensor platform and carboxylated graphitic carbon nitride as signal amplification

Cited by 85 publications

References 52 publications

Sensitive sandwich-type voltammetric immunosensor for breast cancer biomarker HER2 detection based on gold nanoparticles decorated Cu-MOF and Cu2ZnSnS4 NPs/Pt/g-C3N4 composite

Sensitive sandwich-type voltammetric immunosensor for breast cancer biomarker HER2 detection based on gold nanoparticles decorated Cu-MOF and Cu2ZnSnS4 NPs/Pt/g-C3N4 composite

Advancement in biosensors for inflammatory biomarkers of SARS-CoV-2 during 2019–2020

Multimodal/Multifunctional Nanomaterials in (Bio)electrochemistry: Now and in the Coming Decade

Contact Info

Product

Resources

About