Valerophenone synthase-like chalcone synthase homologues in Humulus lupulus

Novák, Petr; Matoušek, Jaroslav; Břı́za, J.

doi:10.1023/a:1024326102694

Cited by 22 publications

(18 citation statements)

References 20 publications

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“…Both genes were mapped to LG08, together with the other three previously mapped chalcone synthase-like genes (chs2, chs3 and chs4). Linkage analysis established that the vps gene is linked to the three chalcone synthase-like genes as was suggested by Novak et al (2003), assuming that the vps gene is part of the same gene cluster as chs3 and chs4. However, none of the chs or vps genes mapped with our detected QTLs.…”

Section: Qtl Analysismentioning

confidence: 74%

“…Valerophenone synthase (VPS) is involved in the first steps of biosynthesis of bitter resins and was the first chalcone synthases (CHS) homologue to be cloned (Paniego et al 1999;Okada and Ito 2001). Four other chs homologues were later isolated from hop: chs_H1, with enzymatic activity in the biosynthesis of bitter acids (Matoušek et al 2002) and chs2, chs4 and chs3, with decreased or no enzymatic activity (Novak et al 2003;Okada et al 2004). However, it has been suggested that more genes and regulatory factors are involved in the biosynthesis of alpha acids (Matoušek et al 2005).…”

Section: Introductionmentioning

confidence: 99%

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Identification of QTLs for alpha acid content and yield in hop (Humulus Lupulus L.)

et al. 2009

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Amplified fragment length polymorphism (AFLP) and microsatellite (SSR) markers were applied to a segregation population of 111 genotypes derived from a pseudo-testcross of hop (Humulus lupulus L.) in order to detect quantitative trait loci (QTLs) for alpha-acid content and yield traits. A total of 199 markers (150 AFLPs, 43 SSRs, one hypothetical sex marker, five chs genes) were located on the 20 linkage groups (LGs) of the maternal and paternal maps, covering 706 and 616 cM, respectively. Due to the presence of 16 common biparental SSR markers, homology of seven LGs between parental maps could be inferred. The progeny segregated quantitatively for alpha-acid content and yield determined in the years from 2002-2006. A total of 13 putative QTLs for alpha acid content, 13 for dry cone weight and 18 for harvest index were identified on the two maps across years.Possible homologies between the detected QTLs on the two maps as well as in different years were established for all three traits. The most promising QTL for alpha acid content was identified on LG03 flanked by two AFLP markers (E-ACC-M-CAA103F*/P-ACA-M-CAC412F). From 13.80 to 36.64% higher content of alpha acids than the averages obtained in different years was observed in plants having both flanking markers. The candidate region for further characterization of QTLs for yield traits was located on LG01 where the putative QTLs for harvest index were detected on both maps in each of the 5 years. The QTLs identified represent an important improvement in alpha acids MAS and the first step towards marker-assisted breeding for hop yield.

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Section: Qtl Analysismentioning

confidence: 74%

Section: Introductionmentioning

confidence: 99%

Identification of QTLs for alpha acid content and yield in hop (Humulus Lupulus L.)

et al. 2009

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“…Chalcone synthase (CHS; EC 2.3.1.74) then catalyzes the condensation of p -coumaroyl-CoA with malonyl-CoA to form naringenin chalcone (NC). A trichome-specific CHS gene, CHS_H1 , has been identified from hops ( 24 ). NC is then prenylated by HlPT1L, and further methylated by an O -methyltransferase (HlOMT1) to form XN ( 20 , 23 , 25 ).…”

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confidence: 99%

Noncatalytic chalcone isomerase-fold proteins in Humulus lupulus are auxiliary components in prenylated flavonoid biosynthesis

Ban

Qin

Mitchell

et al. 2018

Proc. Natl. Acad. Sci. U.S.A.

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SignificanceHere, we identify two noncatalytic chalcone isomerase-fold proteins, which are critical for high-efficiency prenylchalcone production in Humulus lupulus. Our results provide insights into their evolutionary development from the ancestral noncatalytic fatty acid-binding chalcone isomerase-fold proteins to specialized auxiliary proteins supporting flavonoid biosynthesis in plants, and open up the possibility of producing high-value plant prenylchalcones using heterologous systems.

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“…An exon 2-specific DNA fragment (460 bp) of the VPS gene was used as a probe. Two other VPS homologous chalcone synthase genes (CHS4 and CHS_H1) are also expressed in lupulin glands (Okada et al 2004), and thus, to avoid cross hybridization, hybridization and washing were performed at 65°C (Ishii et al 1997, Novák et al 2003. NBT/BCIP (4-nitroblue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate) color detection was used, with a violet-purple color indicating the hybridizing signals (closed arrowheads in Figure 2).…”

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confidence: 99%

Two distinct phase of glandular trichome development in hop (Humulus lupulus L.)

Sugiyama

Oda

Kurosaki

2006

Plant Biotechnology

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Hop bitter resins and essential oils are biosynthesized and accumulated in lupulin glands of hop (Humulus lupulus L.). Valerophenone synthase (VPS) is involved in the first steps of biosynthesis of bitter resins, and the VPS gene is specifically expressed in lupulin glands on bracteoles and leaves. In this study, spatial and temporal expression of the VPS gene was studied using in-situ hybridization. No gene signals were observed in the early stage of lupulin gland development, but strong expression was observed when the cuticle was slightly detached from the glandular head cells. Expression levels were remarkably reduced with accumulation of hop bitter resins and essential oils in the sub-cuticular space. The findings suggest that development of lupulin glands is strictly divided into a growth phase and biosyntheticsecretory phase.

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Valerophenone synthase-like chalcone synthase homologues in Humulus lupulus

Cited by 22 publications

References 20 publications

Identification of QTLs for alpha acid content and yield in hop (Humulus Lupulus L.)

Identification of QTLs for alpha acid content and yield in hop (Humulus Lupulus L.)

Noncatalytic chalcone isomerase-fold proteins in Humulus lupulus are auxiliary components in prenylated flavonoid biosynthesis

Two distinct phase of glandular trichome development in hop (Humulus lupulus L.)

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