Vaginotypes of the human vaginal microbiome

Mancabelli, Leonardo; Tarracchini, Chiara; Milani, Christian; Lugli, Gabriele Andrea; Fontana, Federico; Turroni, Francesca; Sinderen, Douwe van; Ventura, Marco

doi:10.1111/1462-2920.15441

Cited by 31 publications

(23 citation statements)

References 67 publications

(112 reference statements)

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“…METAnnotatorX2 ( 56 ) was used with default settings, 50,000 input reads, and human read filtering. We retrieved from the public data set a representative complete subset of data sequenced on an Illumina MiSeq platform ( 57 ). The strain tracking analysis was instead carried out using all publicly available genomes of L. crispatus , L. iners , L. gasseri , and L. jensenii through the StrainGE software ( https://github.com/broadinstitute/StrainGE ), with default parameters, against 80 publicly available vaginal shotgun sequencing samples (selected based on its high-depth sequencing) and downloaded from the Sequence Reads Archive (SRA) of NCBI (Table S1).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of Modulatory Activities of Lactobacillus crispatus Strains in the Context of the Vaginal Microbiota

et al. 2022

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Section: Methodsmentioning

confidence: 99%

Evaluation of Modulatory Activities of Lactobacillus crispatus Strains in the Context of the Vaginal Microbiota

et al. 2022

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“…The vaginal microbiota change not only during the female menstrual cycle but also during the reproductive process (36). The vaginal microbiota are closely related to women's health, especially APOs.…”

Section: The Vaginal Microbiota and Aposmentioning

confidence: 99%

The Role of Microbiota in Infant Health: From Early Life to Adulthood

et al. 2021

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From early life to adulthood, the microbiota play a crucial role in the health of the infant. The microbiota in early life are not only a key regulator of infant health but also associated with long-term health. Pregnancy to early life is the golden time for the establishment of the infant microbiota, which is affected by both environmental and genetic factors. Recently, there is an explosion of the studies on the role of microbiota in human diseases, but the application to disease or health is relatively limited because many aspects of human microbiota remain controversial, especially about the infant microbiota. Therefore, a critical and conclusive review is necessary to understand fully the relationship between the microbiota and the health of infant. In this article, we introduce in detail the role of microbiota in the infant from pregnancy to early life to long-term health. The main contents of this article include the relationship between the maternal microbiota and adverse pregnancy outcomes, the establishment of the neonatal microbiota during perinatal period and early life, the composition of the infant gut microbiota, the prediction of the microbiota for long-term health, and the future study directions of microbiota.

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“…Lactobacillus species act to suppress anaerobes like Gardnerella , Streptococcus , Prevotella , Ureaplasma and others related to infections such as bacterial vaginosis. The vaginal microbiota, examined via 16S rRNA sequencing, is often classified into community state types (CSTs) based on which species dominates the microbiome, i.e., Lactobacillus crispatus (CST I), Lactobacillus gasseri (CST II), Lactobacillus iners (CST III), Lactobacillus jensenii (CST V) and CST IV (diverse mixture of anaerobic bacteria); with recent studies based on metagenomics sequencing expanding these groups ( Mancabelli et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Neutrophils Dominate the Cervical Immune Cell Population in Pregnancy and Their Transcriptome Correlates With the Microbial Vaginal Environment

et al. 2022

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The cervicovaginal environment in pregnancy is proposed to influence risk of spontaneous preterm birth. The environment is shaped both by the resident microbiota and local inflammation driven by the host response (epithelia, immune cells and mucous). The contributions of the microbiota, metabolome and host defence peptides have been investigated, but less is known about the immune cell populations and how they may respond to the vaginal environment. Here we investigated the maternal immune cell populations at the cervicovaginal interface in early to mid-pregnancy (10–24 weeks of gestation, samples from N = 46 women), we confirmed neutrophils as the predominant cell type and characterised associations between the cervical neutrophil transcriptome and the cervicovaginal metagenome (N = 9 women). In this exploratory study, the neutrophil cell proportion was affected by gestation at sampling but not by birth outcome or ethnicity. Following RNA sequencing (RNA-seq) of a subset of neutrophil enriched cells, principal component analysis of the transcriptome profiles indicated that cells from seven women clustered closely together these women had a less diverse cervicovaginal microbiota than the remaining three women. Expression of genes involved in neutrophil mediated immunity, activation, degranulation, and other immune functions correlated negatively with Gardnerella vaginalis abundance and positively with Lactobacillus iners abundance; microbes previously associated with birth outcome. The finding that neutrophils are the dominant immune cell type in the cervix during pregnancy and that the cervical neutrophil transcriptome of pregnant women may be modified in response to the microbial cervicovaginal environment, or vice versa, establishes the rationale for investigating associations between the innate immune response, cervical shortening and spontaneous preterm birth and the underlying mechanisms.

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Vaginotypes of the human vaginal microbiome

Cited by 31 publications

References 67 publications

Evaluation of Modulatory Activities of Lactobacillus crispatus Strains in the Context of the Vaginal Microbiota

Evaluation of Modulatory Activities of Lactobacillus crispatus Strains in the Context of the Vaginal Microbiota

The Role of Microbiota in Infant Health: From Early Life to Adulthood

Neutrophils Dominate the Cervical Immune Cell Population in Pregnancy and Their Transcriptome Correlates With the Microbial Vaginal Environment

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