Evaluation of Modulatory Activities of Lactobacillus crispatus Strains in the Context of the Vaginal Microbiota

Argentini, Chiara; Fontana, Federico; Alessandri, Giulia; Lugli, Gabriele Andrea; Mancabelli, Leonardo; Ossiprandi, Maria Cristina; Sinderen, Douwe van; Ventura, Marco; Milani, Christian; Turroni, Francesca

doi:10.1128/spectrum.02733-21

Cited by 16 publications

(13 citation statements)

References 67 publications

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“…The 24 h incubation of CFs of L. brevis KNUAS017 considerably inhibited the growth of S. aureus and E. coli (51.59% and 61.96%), followed by L. sakei KNUAS019 (39.54% and 58.42%) and L. graminis KNUAS018 (41.56% and 47.19%), respectively. Similarly, the earlier study reported that L. crispatus strain exhibited significant inhibitory activity among the other LAB stains when co-cultured with a different bacterial pathogen, which might have a higher content of bacteriocin [ 70 ]. Similarly, several studies confirmed the antibacterial efficiency of LAB [ 58 , 71 ].…”

Section: Resultsmentioning

confidence: 86%

Antioxidant and Antibacterial Effects of Potential Probiotics Isolated from Korean Fermented Foods

Sathiyaseelan

Saravanakumar

Han

et al. 2022

IJMS

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A total of sixteen bacterial strains were isolated and identified from the fourteen types of Korean fermented foods that were evaluated for their in vitro probiotic potentials. The results showed the highest survivability for Bacillus sp. compared to Lactobacillus sp. in simulated gastric pH, and it was found to be maximum for B. inaquosorum KNUAS016 (8.25 ± 0.08 log10 CFU/mL) and minimum for L. sakei KNUAS019 (0.8 ± 0.02 log10 CFU/mL) at 3 h of incubation. Furthermore, B. inaquosorum KNUAS016 and L. brevis KNUAS017 also had the highest survival rates of 6.86 ± 0.02 and 5.37 ± 0.01 log10 CFU/mL, respectively, in a simulated intestinal fluid condition at 4 h of incubation. The percentage of autoaggregation at 6 h for L. sakei KNUAS019 (66.55 ± 0.33%), B. tequilensis KNUAS015 (64.56 ± 0.14%), and B. inaquosorum KNUAS016 (61.63 ± 0.19%) was >60%, whereas it was lower for L. brevis KNUAS017 (29.98 ± 0.09%). Additionally, B. subtilis KNUAS003 showed higher coaggregation at 63.84 ± 0.19% while B. proteolyticus KNUAS001 found at 30.02 ± 0.33%. Among them, Lactobacillus sp. showed the best non-hemolytic activity. The highest DPPH and ABTS radical scavenging activity was observed in L. sakei KNUAS019 (58.25% and 71.88%). The cell-free supernatant of Lactobacillus sp. considerably inhibited pathogenic growth, while the cell-free supernatant of Bacillus sp. was moderately inhibited when incubated for 24 h. However, the overall results found that B. subtilis KNUAS003, B. proteolyticus KNUAS012, L. brevis KNUAS017, L. graminis KNUAS018, and L. sakei KNUAS019 were recognized as potential probiotics through different functional and toxicity assessments.

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Section: Resultsmentioning

confidence: 86%

Antioxidant and Antibacterial Effects of Potential Probiotics Isolated from Korean Fermented Foods

Sathiyaseelan

Saravanakumar

Han

et al. 2022

IJMS

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“…These findings, although obtained in in vitro tests, underline the potential of L. crispatus strains as beneficial microbes and strengthen the identification of L. crispatus species as a hallmark of vaginal eubiosis [ 34 , 35 ]. Therefore, strains belonging to this species should be further characterized for their safety in cellular models and in in vivo studies, in the perspective of employing probiotic strains to prevent VVC and RVVC or to restore vaginal homeostasis.…”

Section: Discussionmentioning

confidence: 62%

Vaginal Lactobacillus Impair Candida Dimorphic Switching and Biofilm Formation

et al. 2022

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Lactobacillus spp. generally dominate the vaginal microbiota and prevent pathogen adhesion and overgrowth, including Candida spp., by various mechanisms. Although Candida spp. can be commensal, in certain conditions they can become pathogenic, causing vulvovaginal candidiasis. The insurgence of candidiasis is related to the expression of Candida virulence factors, including morphologic switching and biofilm formation. Germ tubes, pseudohyphae, and hyphae promote Candida tissue invasion, biofilms increase persistence and are often resistant to antifungals and host immune response. Here, we explored the inhibitory activity of vaginal Lactobacillus strains belonging to Lactobacillus crispatus, Lactobacillus gasseri, Limosilactobacillus vaginalis, and Lactiplantibacillus plantarum species towards Candida virulence factors. With the aim to investigate the interrelation between mode of growth and functionality, supernatants were collected from lactobacilli planktonic cultures and, for the first time, from adherent ones, and were evaluated towards Candida dimorphic switching and biofilm. Candida biofilms were analyzed by multiple methodologies, i.e., crystal violet staining, MTT assay, and confocal microscopy. Lactobacillus supernatants reduce Candida switching and biofilm formation. Importantly, L. crispatus supernatants showed the best profile of virulence suppression, especially when grown in adherence. These results highlight the role of such species as a hallmark of vaginal eubiosis and prompt its employment in new probiotics for women’s health.

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“…However, changes in the natural microbiota of the urogenital system can be caused by many factors and are associated with age [ 26 ], medical treatment [ 7 ], diet, or diabetes [ 27 ]. Although there are many scientific reports on the antibacterial properties of Lactobacillus against pathogens, these data mainly concern strains isolated from the genital tract or food [ 8 , 28 , 29 ]. The innovativeness of these studies is based on the understanding of the interactions between microorganisms constantly present in the urinary tract and uropathogens.…”

Section: Discussionmentioning

confidence: 99%

Organic Acids Secreted by Lactobacillus spp. Isolated from Urine and Their Antimicrobial Activity against Uropathogenic Proteus mirabilis

et al. 2022

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The natural microbiota of the urinary tract includes Lactobacillus spp., which secrete molecules with antimicrobial properties and have antagonistic activity against many pathogens. This paper focuses on the antibacterial effect of Lactobacillus strains isolated from urine against clinical strains of Proteus mirabilis isolated from kidney stones and from urine with coexisting urolithiasis. The study involved analyzing the main antimicrobial molecules secreted by Lactobacillus. In order to indicate which agent had the strongest antimicrobial effect, the supernatants were made alkaline and treated with catalase and high temperature. Both treated and untreated supernatants were analyzed for their activity. Exposing uropathogens to all untreated cell-free supernatants of Lactobacillus significantly reduced their growth, and it was established that these properties were related to organic acid secretion by these strains. Using LC–MS/MS and spectrophotometric techniques, lactic, citric, and succinic acids were determined qualitatively and quantitatively. The influence of these acids on the P. mirabilis growth and biofilm formation and their influence on membrane permeability were also investigated. The results indicate that organic acids secreted by Lactobacillus strains have a high antibacterial potential and could be used as novel agents in the treatment of urinary tract infections caused by P. mirabilis.

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Evaluation of Modulatory Activities of Lactobacillus crispatus Strains in the Context of the Vaginal Microbiota

Abstract: The human vaginal microbiota includes all microorganisms that colonize the vaginal tract. In this context, a vaginal microbiota dominated by Lactobacillus and specifically by Lactobacillus crispatus is considered a hallmark of health.

Cited by 16 publications

References 67 publications

Antioxidant and Antibacterial Effects of Potential Probiotics Isolated from Korean Fermented Foods

Antioxidant and Antibacterial Effects of Potential Probiotics Isolated from Korean Fermented Foods

Vaginal Lactobacillus Impair Candida Dimorphic Switching and Biofilm Formation

Organic Acids Secreted by Lactobacillus spp. Isolated from Urine and Their Antimicrobial Activity against Uropathogenic Proteus mirabilis

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