Vaginal lactic acid bacteria in the mare: evaluation of the probiotic potential of native Lactobacillus spp. and Enterococcus spp. strains

Fraga, Martín; Perelmuter, Karen; Delucchi, Luis; Cidade, Esther; Zunino, Pablo

doi:10.1007/s10482-007-9180-4

Cited by 27 publications

(34 citation statements)

References 33 publications

(33 reference statements)

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“…As the first step for LAB colonization is adhesion to the epithelial cell surface and formation of a biofilm (Ocana and Nader-Macias, 2002), there is a significant correlation between bacterial adhesion to the host cell surface and probiotic ability (Del Re et al, 1998, 2000Makras et al, 2006). In this study, we demonstrated these characteristics in LAB isolates from the intestinal tract of Muscovy duck, consistent with previous reports (Calderon et al, 2007;Mountzouris et al, 2007;Reddy et al, 2007;Fraga et al, 2008;Klose et al, 2010). Although only a few LAB isolates identified showed high levels of hydrophobicity and autoaggregation indices, we demonstrated that these properties are present in probiotics (Espeche et al, 2009).…”

Section: Discussionsupporting

confidence: 91%

Intestinal lactic acid bacteria from Muscovy duck as potential probiotics that alter adhesion factor gene expression

Xie¹,

Bai²,

Xie³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. The purpose of this study was to assess the suitability of lactic acid bacteria (LABs) isolated from Muscovy duck as a potential probiotic. Isolates were identified by targeted polymerase chain reaction and assessed in vitro for probiotic characteristics such as autoaggregation; surface-charge; hydrophobicity; tolerance to acidic pH, bile salts and protease; and expression of genes involved in Caco-2 cell adhesion. The LAB isolates exhibited strong resistance to high bile concentration and acidic pH, produced lactic acid, and bacteriostatic (P < 0.05) were identified as bacilli compared with LAB isolates of cocci. Additionally, the LAB isolates showed high sensitivity to penicillin and tetracycline antibiotics, while they were resistant to ofloxacin, Macrodantin, and cotrimoxazole. The level of F-actin mRNA increased in the groups treated with CM3, Salmonella enterica, and CM3 + S. enterica (P < 0.0001, P < 0.05 and P < 0.05 ). The level of cell adhesion molecule (CAM) and E-cadherin (E-cad) mRNA expression was significantly lower in the treatment group (P < 0.05 for both) than in the control. The F-actin, CAM, and E-cad mRNA levels were significantly lower in the S. enterica and CM3 + S. enterica groups (P < 0.01) than in the CM3 group. Among these, RNA levels were higher in the CM3 + S. enterica than S. enterica group. These results indicate that the natural duck gut microflora is an excellent source for probiotic bacteria and can facilitate the establishment of criteria to select probiotic strains for the prevention of diarrhea.

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Section: Discussionsupporting

confidence: 91%

Intestinal lactic acid bacteria from Muscovy duck as potential probiotics that alter adhesion factor gene expression

Xie¹,

Bai²,

Xie³

et al. 2015

Genet. Mol. Res.

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“…The ability to adhere to the epithelial cells and coaggregation ability are considered as important criterions for in vitro probiotic selection (15,33,34). Our results are in agreement with those of several authors (10,14).…”

Section: Discussionmentioning

confidence: 99%

“…H 2 O 2 , lactic acid and other organic acids produced by LAB are frequently associated with this activity in vitro (15). The inhibition of urogenital infections increases the relevance of these wild strains for use in probiotic products (39).…”

Section: Discussionmentioning

confidence: 99%

“…From vaginal fluid, samples were collected with sterile cotton swabs inserted into the vagina, rotated a few turns along the vaginal sidewall, and allowed to absorb for few seconds (15). The swabs were immersed in the sterile normal saline, and used for anaerobic (BD Gaspak TM Anaerobic system, USA) culture preparation by streaking on de Man Rogasa Sharpe (MRS) Agar (CONDA, Pronadisa, Spain) plates (16).…”

Section: Methodsmentioning

confidence: 99%

“…Bacterial adhesion to VECs was assessed by microscopy (×100) after staining with 1% of crystal violet. The number of bacteria attached to 50 consecutive VECs smears was counted and VECs Controls smears were made to confirm that the presence of native bacteria was negligible (15). …”

Section: Methodsmentioning

confidence: 99%

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Technological and Probiotic Traits of the Lactobacilli Isolated From Vaginal Tract of the Healthy Women for Probiotic Use

Bouridane

Sifour

Idoui

et al. 2016

Iran. J. Biotechnol.

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Background For biotechnological application, selected lactic acid bacteria strains belonging to the genera Lactobacillus (Lb) are proposed as an alternative to the antibiotics for the prevention and treatment of urogenital tract infections. Objectives Isolating and selecting vaginal lactobacilli strains for probiotic use based on their technological and probiotic aptitudes. Materials and Methods The vaginal isolates were examined for their essential characteristics as the potential probiotic such as low pH tolerance, bile-salt and simulated human intestinal fluid (SIF) resistance, adhesion to the vaginal epithelial cells (VECs), aggregation and coaggregation, surface hydrophobicity, antimicrobial activity, acid production, antibiotic resistance, and resistance to spermicides. The best strain was identified by PCR. Results From 70 lactobacilli isolates and according to the 16 rDNA sequences, isolates B6 and B10 showed the closest homology (99%) to the Lb. gasseri and Lb. plantarum respectively. They produced hydrogen peroxide (H2O2), tolerant to acid, bile, simulated human intestinal fluid, present a strong adhesion, highest percentages of aggregation, and antibacterial activity. These strains are resistant to the spermicide and actively acidify the growth medium. Conclusions Strains Lb. plantarum B10 and Lb. gasseri B6 have a strong potential probiotic confirming their value as a tool for prevention against urinary and vaginal infections.

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Isolation of lactic acid bacteria from the reproductive tract of mares as potentially beneficial strains to prevent equine endometritis

Silva,

Castañares,

Mouguelar

et al. 2024

Vet Res Commun

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Endometritis, the infection and/or in ammation of the endometrium, is the leading cause of subfertility in mares, and therefore responsible for major economic losses in the horse industry worldwide. It is generally treated with uterine lavages combined with ecbolic agents and local or systemic antibiotics. However, since antibiotic overuse has been associated with antimicrobial resistance in mares with persistent endometritis, new prevention and treatment alternatives are needed. One such alternative could be the use of probiotic lactic acid bacteria (LAB) isolated from the host. Thanks to their species speci city, indigenous microbiota may restore ecological equilibrium within the host, and therefore, help prevent infections and improve physiological functions. In the present study, 257 bacterial strains were isolated from 77 healthy mares, and 88.76% (n = 228) of them were phenotypically classi ed as LAB. Within this group, 65.79% were able to inhibit at least one strain from each of the genera that most commonly cause equine endometritis (S. zooepidemicus, E. coli, and Staphylococcus spp.). Five strains (RCE11, RCE20, RCE91, RCE99, and RCE167) were selected on the basis of their bene cial properties: ability to autoaggregate and adhere to equine epithelial cells, high inhibition of and co-aggregation with all the bacteria isolated from clinical cases of endometritis evaluated, and negative co-inhibition between one another. All ve were nally identi ed as Enterococcus spp., namely E. faecium (two strains), E. hirae (two strains), and E. gallinarum (one strain). Further studies will assess their safety and biotechnological potential for the design of a multi-strain probiotic formula to prevent equine endometritis.

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Vaginal lactic acid bacteria in the mare: evaluation of the probiotic potential of native Lactobacillus spp. and Enterococcus spp. strains

Cited by 27 publications

References 33 publications

Intestinal lactic acid bacteria from Muscovy duck as potential probiotics that alter adhesion factor gene expression

Intestinal lactic acid bacteria from Muscovy duck as potential probiotics that alter adhesion factor gene expression

Technological and Probiotic Traits of the Lactobacilli Isolated From Vaginal Tract of the Healthy Women for Probiotic Use

Isolation of lactic acid bacteria from the reproductive tract of mares as potentially beneficial strains to prevent equine endometritis

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