Vaginal Gel Component Hydroxyethyl Cellulose Significantly Enhances the Infectivity of
            <i>Chlamydia trachomatis</i>
            Serovars D and E

Raffai, Tímea; Burián, Katalin; Janovák, László; Bogdanov, Anita; Hegemann, Johannes H.; Endrész, Valéria; Virók, Dezső

doi:10.1128/aac.02034-18

Cited by 6 publications

(6 citation statements)

References 16 publications

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“…Incorporating microbicides into vaginal gels is a well-accepted strategy for inhibiting sexually transmitted disease transmission including HSV-2 transmission [9][10][11]. We also showed that even basic components of the vaginal gels, such as the gelling agent hydroxyethyl cellulose can also significantly influence the replication of sexually transmitted pathogens such as Chlamydia trachomatis [12].…”

Section: Introductionmentioning

confidence: 81%

Correlation between detergent activity and anti-herpes simplex virus-2 activity of commercially available vaginal gels

et al. 2020

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Objective: Herpes simplex virus-2 (HSV-2) infections are almost exclusively sexually transmitted. The presence of vaginal gels during sexual activity may have a significant positive or negative impact on viral transmission. Therefore we investigated three off-the-shelf vaginal lubricants and one pH restoring gel to evaluate their impact on HSV-2 replication.Results: HeLa cells were infected with untreated virions and virions incubated with the particular gels. The accumulation of viral genomes was monitored by quantitative PCR (qPCR) method at 24 h post infection. Two of the tested gels had no significant effect on HSV-2 replication at the maximum applied concentration, while two had a strong inhibitory effect (~ 98% reduction of replication). The replication inhibitory effect was observed at various multiplicity of infection (MOI 0.4-6.4) and the two inhibitory gels were also capable of inhibiting the HSV-2 induced cytopathic effect on HeLa cells. The surface tension decreasing activity-an indication of detergent activity-was strongly correlated with the anti-HSV-2 activity of the gels (R 2 : 0.88). Our results indicate that off-the-shelf vaginal gels have a markedly different anti-HSV-2 activity that may influence HSV-2 transmission.

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Section: Introductionmentioning

confidence: 81%

Correlation between detergent activity and anti-herpes simplex virus-2 activity of commercially available vaginal gels

et al. 2020

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“…The titer of infectious elementary bodies (EBs) was determined by indirect immunofluorescence assay and was expressed in inclusion forming unit/mL (IFU/mL) [18]. HeLa cells were maintained in minimal essential medium (MEM) comprising 10% fetal bovine serum, 2 mM L-glutamine, 1 × nonessential amino acids, 1 × MEM vitamins, 25 μg/mL gentamicin, and 1 μg/mL fungizone [19].…”

Section: Methodsmentioning

confidence: 99%

Bioactive Compounds of Nigella Sativa Essential Oil as Antibacterial Agents against Chlamydia Trachomatis D

et al. 2019

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Urogenital tract infection caused by obligate intracellular bacterium Chlamydia trachomatis D (CtrD) is a leading cause of sexually transmitted diseases. Essential oil (EO) of Nigella sativa has a broad antimicrobial spectrum. The aim of this study was to evaluate the antimicrobial activity of the bioactive compounds (p-cymene, thymoquinone, carvacrol, and thymol) of N. sativa EO against CtrD. The cytotoxic effects of the compounds were determined by MTT assay. In order to quantify the anti-chlamydial activity of the compounds, HeLa cells were infected with CtrD or CtrD treated previously with the compounds. The titer of the infectious CtrD was determined by indirect immunofluorescence assay. The minimum inhibitory concentrations of the compounds were evaluated by direct quantitative PCR. None of the compounds showed a cytotoxic effect on HeLa cells in the concentrations tested. According to the immunofluorescence assay, all of the compounds significantly inhibited the growth of CtrD. The quantitative PCR revealed that the minimum concentration that exerted anti-chlamydial activity was 3.12 µM in the case of thymoquinone and p-cymene, while that of carvacrol and thymol was 6.25 µM. Therefore, it can be concluded that bioactive compounds of N. sativa EO could be used as effective antimicrobial agents against CtrD.

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“…Direct PCR and direct qPCR eliminate the purification step, significantly shortening the protocol, but the inhibitory effect of the direct sample can be present. Previously, direct qPCR methods have been successfully applied to monitor Chlamydia and herpes simplex virus-2 growth and the antimicrobial effects of various compounds [6][7][8][9][10][11]. In this study, we want to leave out the DNA purification step and develop a direct qPCR detection method that is suitable to detect Mycoplasma contamination within U937 cell cultures.…”

Section: Introductionmentioning

confidence: 99%

Direct qPCR is a sensitive approach to detect Mycoplasma contamination in U937 cell cultures

et al. 2019

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Objective: We aim to directly detect Mycoplasma DNA in a U937 suspension cell culture without using DNA purification. In order to make Mycoplasma contamination monitoring easier, we optimized a commercially available quantitative PCR (qPCR)-based detection kit. We compared the sensitivity of direct qPCR against qPCR with a purified DNA template. Results: Our findings indicate that qPCR worked optimally with a 6 μl sample volume and a 52 °C annealing-extension temperature. We were able to decrease the annealing-extension step time from 60 to 20 s without any major decrease in reaction sensitivity. The total cycle time of optimized direct qPCR was 65 min. The optimized qPCR protocol was used to detect Mycoplasma DNA before and after DNA purification. Our findings indicate that direct qPCR had a higher sensitivity than regular qPCR. Ct levels produced by direct qPCR with 6 μl templates were almost identical to Ct levels produced by regular qPCR with DNA purified from a 60 μl cell culture sample (23.42 vs 23.49 average Ct levels, respectively). The optimized direct qPCR protocol was successfully applied to monitor the elimination of Mycoplasma contamination from U937 cell cultures.

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Vaginal Gel Component Hydroxyethyl Cellulose Significantly Enhances the Infectivity of Chlamydia trachomatis Serovars D and E

Cited by 6 publications

References 16 publications

Correlation between detergent activity and anti-herpes simplex virus-2 activity of commercially available vaginal gels

Correlation between detergent activity and anti-herpes simplex virus-2 activity of commercially available vaginal gels

Bioactive Compounds of Nigella Sativa Essential Oil as Antibacterial Agents against Chlamydia Trachomatis D

Direct qPCR is a sensitive approach to detect Mycoplasma contamination in U937 cell cultures

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