Vacuolar protein in apical and flower-petal cells

Shumway, L. K.; Cheng, Vincent Wing-Sang; Ryan, Clarence A.

doi:10.1007/bf00384766

Cited by 19 publications

(3 citation statements)

References 10 publications

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“…These findings suggest that spherical bodies observed in the vacuole are not anthocyanic vacuolar inclusions. Protein bodies, which are spherical, have been observed in vacuoles in some petals including petunia, tomato, tobacco (Shumway et al, 1972), and Rhododendron (Schneider, 1972). However, intravacuolar protein bodies have not been observed in petals in carnation (Smith et al, 1992) and rose (Yamada et al, 2009b).…”

Section: Discussionmentioning

confidence: 99%

Cell Division and Expansion in Petals during Flower Development and Opening in <i>Eustoma grandiflorum</i>

2016

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Section: Discussionmentioning

confidence: 99%

Cell Division and Expansion in Petals during Flower Development and Opening in <i>Eustoma grandiflorum</i>

2016

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“…A similar uptake of DIP organelles into vacuoles in root tip cells also seems to be developmentally regulated. It is possible that this process may be related to the formation of what have been termed vacuolar protein bodies in vegetative tissues (Greyson and Mitchell, 1969;Shumway et al, 1972). As little or no DIP labeling was detected in the PSV tonoplast, and little or no labeling for ␦-TIP or ␣-TIP (PSV tonoplast markers) was detected around or in the crystalloid, we hypothesize that the DIP organelles in the cytoplasm are surrounded by a membrane that fuses with the PSV tonoplast to deliver the internal, DIP-containing membranes to the vacuole interior.…”

Section: Discussionmentioning

confidence: 99%

Biogenesis of the Protein Storage Vacuole Crystalloid

Jiang

Phillips

Rogers

et al. 2000

The Journal of Cell Biology

168

221

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We identify new organelles associated with the vacuolar system in plant cells. These organelles are defined biochemically by their internal content of three integral membrane proteins: a chimeric reporter protein that moves there directly from the ER; a specific tonoplast intrinsic protein; and a novel receptor-like RING-H2 protein that traffics through the Golgi apparatus. Highly conserved homologues of the latter are expressed in animal cells. In a developmentally regulated manner, the organelles are taken up into vacuoles where, in seed protein storage vacuoles, they form a membrane-containing crystalloid. The uptake and preservation of the contents of these organelles in vacuoles represents a unique mechanism for compartmentalization of protein and lipid for storage.

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“…Shumway et al (15,16) observed that protein bodies are present in tomato leaves which contain proteinase inhibitor I and later (17) indirectly identified the inhibitor in the protein bodies using immunological methods combined with electron microscope techniques. In this study we have isolated vacuoles from tomato leaves that were induced to accumulate inhibitors I and II and directly confirm that these two proteinase inhibitors are located in vacuoles of the leaf cells.…”

mentioning

confidence: 99%

Immunological Identification of Proteinase Inhibitors I and II in Isolated Tomato Leaf Vacuoles

Walker-Simmons¹,

Ryan²

1977

Plant Physiol.

111

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Proteinase inhibitor I has been identified and quantified in isolated vacuoles from tomato (Lycopersicon esculentum) leaves induced to accumulate inhibitors either by wounding or by supplying excised leaves with the wound hormone, proteinase inhibitor-inducing factor. Proteinase inhibitor II was also identified in the vacuoles but not quantified. Control vacuoles were prepared from unwounded plants that did not contain inhibitors. Vacuole with a 17-hr day. Excised leaves were induced to accumulate proteinase inhibitors by supplying them with a crude PIIF solution (13) for 20 min. The leaves were rinsed and supplied with water under 1,000 ft-c for 72 hr at 31 C. Leaves from young intact plants were induced to accumulate inhibitors by crushing the center of the lower leaves between a rubber stopper (No. 000) and a flat file. This was repeated for 2 consecutive days to ensure maximal accumulation when vacuoles were prepared from the leaves.Proteinase inhibitor I was quantified by the immunoradial diffusion method described by Ryan (12). Purified proteinase inhibitor I from tomato leaves (9) was used as the standard. Inhibitor II was identified by this method but was not quantified because no standard tomato leaf inhibitor II was available at the time of assay.The number of cells/g in leaf tissue was estimated from measurements of the volume of epidermal, mesophyll, and palisade cells in a young tomato leaf representative of those utilized for this study. An intact leaf was measured and weighed, and the number of cells, based on volume of each type of cell, was calculated for the whole leaf. Approximately 2.5 x 107 cells/g of leaf tissue were calculated for the leaves used in this study.Vacuole isolation was a modification of the method of Wagner and Siegelman (20) and is similar to that of Strobel and Hess (19). Tomato leaves were sterilized in 70% alcohol and shredded into longitudinal strips 1 to 2 mm wide. The strips were floated on a sterile solution of 1.5% (w/v) Cellulysin (Calbiochem) and 0.25 M sucrose adjusted to pH 5.75 with NaOH. The sterile leaf mixture was incubated at room temperature with gentle shaking for about 20 hr in small Petri dishes (60 x 15 mm) each containing 12 ml of solution. After incubation, the shredded leaf solution was collected in a beaker, stirred (10 rpm) with an Omni-Mixer for 30 min, and filtered through glass wool and cheesecloth. The filtered solution was centrifuged in Babcock bottles (Kimble No. 1000) (14) in a one-speed Jalco centrifuge (model 58) for 5 min at approximately 500g. During centrifugation, a vacuole and protoplast mixture floated to the surface and collected in the neck of the Babcock bottle. After centrifugation the vacuole-enriched fraction was gently removed from the neck of the Babcock bottle with a Pasteur pipette. The vacuole-enriched fraction was resuspended in 0.195 M sucrose, which released more vacuoles from the protoplasts, and was centrifuged again. The resuspension and centrifugation process was repeated several times until as much as po...

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Vacuolar protein in apical and flower-petal cells

Cited by 19 publications

References 10 publications

Cell Division and Expansion in Petals during Flower Development and Opening in <i>Eustoma grandiflorum</i>

Cell Division and Expansion in Petals during Flower Development and Opening in <i>Eustoma grandiflorum</i>

Biogenesis of the Protein Storage Vacuole Crystalloid

Immunological Identification of Proteinase Inhibitors I and II in Isolated Tomato Leaf Vacuoles

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