2000
DOI: 10.1016/s0005-2736(00)00130-9
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Vacuolar H+-pyrophosphatase

Abstract: The H(+)-translocating inorganic pyrophosphatase (H(+)-PPase) is a unique, electrogenic proton pump distributed among most land plants, but only some alga, protozoa, bacteria, and archaebacteria. This enzyme is a fine model for research on the coupling mechanism between the pyrophosphate hydrolysis and the active proton transport, since the enzyme consists of a single polypeptide with a calculated molecular mass of 71-80 kDa and its substrate is also simple. Cloning of the H(+)-PPase genes from several organis… Show more

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Cited by 407 publications
(405 citation statements)
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“…Removal of PP i prevents these reactions from reaching equilibrium and plays an important role in maintaining the direction of these reactions (Maeshima, 2000). Arabidopsis encodes six soluble inorganic PPases and three membrane-bound H + -translocating PPases (Schulze et al, 2004;Ferjani et al, 2011).…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Removal of PP i prevents these reactions from reaching equilibrium and plays an important role in maintaining the direction of these reactions (Maeshima, 2000). Arabidopsis encodes six soluble inorganic PPases and three membrane-bound H + -translocating PPases (Schulze et al, 2004;Ferjani et al, 2011).…”
Section: Discussionmentioning
confidence: 99%
“…But generally PPases remove pyrophosphate (PP i ) that is created as a byproduct of anabolic processes such as nucleic acid, protein, and carbohydrate synthesis (Gómez-García et al, 2006). Removal of PP i is necessary to prevent the inhibition of thermodynamically unfavorable reactions (Maeshima 2000). Another function could be to keep cytosolic [PP i ] levels low, since high [PP i ] is toxic (Cooperman et al, 1992).…”
mentioning
confidence: 99%
“…Proton pumping pyrophosphatase (H ϩ -PPase) 1 is an integral membrane protein that utilizes the energy released upon the hydrolysis of PP i to transport protons across the membrane against the electrochemical potential gradient (1)(2)(3). H ϩ -PPases represent a distinct class of ion translocases with no sequence similarity to ubiquitous ATP-energized pumps such as the F-, V-, or P-type ATPases or ABC transporters (4).…”
mentioning
confidence: 99%
“…[1][2][3][4] This is congruent with the distinct localization of H C -PPases in the vacuoles of actively dividing cells of plants. [5][6][7][8][9][10] However, H C -PPase also saliently localizes at the vascular tissues of plants, wherein this protein is predominantly localized at the plasma membrane (PM) of the sieve element companion cell (SE-CC) complexes.…”
mentioning
confidence: 65%