VaCPK20 , a calcium-dependent protein kinase gene of wild grapevine Vitis amurensis Rupr., mediates cold and drought stress tolerance

Dubrovina, Alexandra S.; Kiselev, Konstantin V.; Khristenko, Valeriya S.; Aleynova, Olga A.

doi:10.1016/j.jplph.2015.05.020

Cited by 83 publications

(48 citation statements)

References 54 publications

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“…Complementary DNA was synthesized using a cDNA synthesis kit with Oligo(dT)15 primer (Silex, Moscow, Russia) as described by Aleynova et al (2016). The real-time qPCRs were performed using a real-time PCR kit (Syntol, Moscow, Russia) and an EvaGreen real-time PCR dye (Biotium, Hayward, USA) as described Dubrovina et al (2015).…”

Section: Extraction Of Rna and Reverse Transcription (Rt) Real-time Qmentioning

confidence: 99%

“…It has been shown previously that both somatic DNA mutations and changes in DNA methylation accumulate with the age of plant tissues (Boyko et al 2006, Golubov et al 2010, Kiselev et al 2015a, Ogneva et al 2016). Furthermore, methylated cytosine (5mC) is known to frequently mutate to thymine in the DNA (Ehrlich et al 1986). However, little is known on the relationship between DNA methylation and mutation accumulation in plants.…”

Section: Introductionmentioning

confidence: 99%

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Somatic mutations, DNA methylation, and expression of DNA repair genes in Arabidopsis thaliana treated with 5-azacytidine

Kiselev¹,

Ogneva²,

Dubrovina³

et al. 2019

Biol. plant.

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An inhibitor of DNA methylation 5-azacytidine (5A) is a chemical analog of the nucleoside cytidine. This study investigated the influence of 5A-induced DNA hypomethylation on the accumulation of somatic DNA mutations (nucleotide substitutions, indels) in the Actin2 3′ untranslated region, nuclear internal transcribed spacer ITS1-5.8rRNA-ITS2, and the ribulose-1,5-bisphosphate carboxylase/oxygenase gene of Arabidopsis thaliana and analyzed concurrent changes in the expression of DNA methyltransferase and DNA repair genes. The 5A treatment (20 mg per 100 g of soil) decreased DNA methylation, and the detected 5A-induced demethylation was associated with the up-regulation of the DNA methyltransferase genes: chromomethylase AtCMT3, methyltransferase AtMETI, and domains rearranged methyltransferases AtDRM1 and AtDRM2. Cultivation of plants in the presence of 5A led to a considerable increase in the number of single nucleotide substitutions in the analyzed DNA regions of 5A-treated A. thaliana. The 5A treatment significantly increased the transcriptions of 7 DNA repair genes (endonuclease AtARP, DNA demethylases AtDME and AtDML2, DNA glycosylase AtMBD4, DNA damage-binding protein AtDDB1, and photolyases AtUVR2 and AtUVR3) out of the 17 analyzed genes from the base excision repair, nucleotide excision repair, and photoreactivation pathways. However, 5A decreased the transcription of DNA 3′-phosphatase AtZDP, DNA repair protein AtRad23a, mismatch repair proteins AtMsh2 and AtMsh3. It is possible that the changes in the transcription of the DNA repair genes contributed to the detected increase in the number of single nucleotide substitutions that accumulated in the 5A-treated A. thaliana. Taken together, the data indicate that there is an interaction between the processes of DNA methylation and mutation accumulation.Additional key words: indels, methyltransferase, single nucleotide substitutions. 

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Section: Extraction Of Rna and Reverse Transcription (Rt) Real-time Qmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Somatic mutations, DNA methylation, and expression of DNA repair genes in Arabidopsis thaliana treated with 5-azacytidine

Kiselev¹,

Ogneva²,

Dubrovina³

et al. 2019

Biol. plant.

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“…In rice and Arabidopsis, to our knowledge, only OsCPK7 , OsCPK13 and AtCPK1 have been analysed and implicated in cold stress response (Abbasi et al ; Bohmer & Romeis ; Komatsu et al ; Saijo et al ). In addition, PeCPK10 , in Populus euphratica (Chen et al ), and VaCPK20 , in Vitis amurensis (Dubrovina et al ), were identified as positive regulators of cold stress tolerance, whereas ZmCPK1 , in Zea mays , has been shown to act as a negative regulator of cold stress signalling (Weckwerth et al ). Evidence indicates that CDPKs certainly participate in plant cold stress tolerance.…”

Section: Introductionmentioning

confidence: 99%

Rice calcium‐dependent protein kinase OsCPK17 targets plasma membrane intrinsic protein and sucrose‐phosphate synthase and is required for a proper cold stress response

Almadanim

Alexandre

Rosa

et al. 2017

Plant Cell & Environment

101

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Calcium-dependent protein kinases (CDPKs) are involved in plant tolerance mechanisms to abiotic stresses. Although CDPKs are recognized as key messengers in signal transduction, the specific role of most members of this family remains unknown. Here we test the hypothesis that OsCPK17 plays a role in rice cold stress response by analyzing OsCPK17 knockout, silencing, and overexpressing rice lines under low temperature. Altered OsCPK17 gene expression compromises cold tolerance performance, without affecting the expression of key cold stress-inducible genes. A comparative phosphoproteomic approach led to the identification of six potential in vivo OsCPK17 targets, which are associated with sugar and nitrogen metabolism, and with osmotic regulation. To test direct interaction, in vitro kinase assays were performed, showing that the sucrose phosphate synthase OsSPS4, and the aquaporin OsPIP2;1/OsPIP2;6 are phosphorylated by OsCPK17 in a calcium-dependent manner. Altogether, our data indicates that OsCPK17 is required for a proper cold stress response in rice, likely affecting the activity of membrane channels and sugar metabolism.Rice production is severely affected by different abiotic stresses, including cold. Cold perception is mediated by calcium signals that activate kinases to elicit the adequate cellular response. In this work, we show the involvement of the rice calciumdependent protein kinase 17 (OsCPK17) in such a process. We show that altered OsCPK17 gene expression in transgenic lines affects cold tolerance performance, This article is protected by copyright. All rights reserved.and that OsCPK17 targets proteins are associated with osmotic regulation, and sugar and nitrogen metabolism.

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“…It is the hardiest species within the genus Vitis and can tolerate temperatures as low as -40°C (Jiao et al, 2015;Ma et al, 2010). Therefore, it is a widely used species for grapevine coldhardiness research (Dubrovina et al, 2015;Li et al, 2013) and for hardy cultivar breeding (Zhang et al, 2015). Furthermore, amur grape is resistant to various diseases incited by fungi, such as Uncinula necator, Plasmopara viticola, and Coniothyrium diplodiella (Li et al, 2008;Wan et al, 2007).…”

mentioning

confidence: 99%

Cytological Study of Gender Conversion in Amur Grape

Shen¹,

Liu²,

Ke³

et al. 2018

J. Amer. Soc. Hort. Sci.

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Amur grape (Vitis amurensis) is a dioecious species. To elucidate the time of and reason for pistil abortion in male amur grape from the perspective of cytology, we observed the sections of pistil of a male line during its development using optical and transmission electron microscopes. The abnormity in the morphology of nucellar cell and the development of various organelles appeared before the abnormity of functional megaspore mitosis. Programmed cell death (PCD) of the nucellar cells might be an important reason for mitosis disorder, leading to the abortion of pistil in male flower. However, the abortion can be eliminated by forchlorfenuron treatment, resulting in the recovery of functional pistil in male amur grape. This study provides cytological information on the gender conversion mechanism in male amur grape, which can promote gender determination studies in Vitis species.

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VaCPK20 , a calcium-dependent protein kinase gene of wild grapevine Vitis amurensis Rupr., mediates cold and drought stress tolerance

Cited by 83 publications

References 54 publications

Somatic mutations, DNA methylation, and expression of DNA repair genes in Arabidopsis thaliana treated with 5-azacytidine

Somatic mutations, DNA methylation, and expression of DNA repair genes in Arabidopsis thaliana treated with 5-azacytidine

Rice calcium‐dependent protein kinase OsCPK17 targets plasma membrane intrinsic protein and sucrose‐phosphate synthase and is required for a proper cold stress response

Cytological Study of Gender Conversion in Amur Grape

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