Rice calcium‐dependent protein kinase OsCPK17 targets plasma membrane intrinsic protein and sucrose‐phosphate synthase and is required for a proper cold stress response

Abstract

Calcium-dependent protein kinases (CDPKs) are involved in plant tolerance mechanisms to abiotic stresses. Although CDPKs are recognized as key messengers in signal transduction, the specific role of most members of this family remains unknown. Here we test the hypothesis that OsCPK17 plays a role in rice cold stress response by analyzing OsCPK17 knockout, silencing, and overexpressing rice lines under low temperature. Altered OsCPK17 gene expression compromises cold tolerance performance, without affecting the expression of key cold stress-inducible genes. A comparative phosphoproteomic approach led to the identification of six potential in vivo OsCPK17 targets, which are associated with sugar and nitrogen metabolism, and with osmotic regulation. To test direct interaction, in vitro kinase assays were performed, showing that the sucrose phosphate synthase OsSPS4, and the aquaporin OsPIP2;1/OsPIP2;6 are phosphorylated by OsCPK17 in a calcium-dependent manner. Altogether, our data indicates that OsCPK17 is required for a proper cold stress response in rice, likely affecting the activity of membrane channels and sugar metabolism.Rice production is severely affected by different abiotic stresses, including cold. Cold perception is mediated by calcium signals that activate kinases to elicit the adequate cellular response. In this work, we show the involvement of the rice calciumdependent protein kinase 17 (OsCPK17) in such a process. We show that altered OsCPK17 gene expression in transgenic lines affects cold tolerance performance, This article is protected by copyright. All rights reserved.and that OsCPK17 targets proteins are associated with osmotic regulation, and sugar and nitrogen metabolism.

Genome-wide survey and expression analysis of calcium-dependent protein kinase (CDPK) in grass Brachypodium distachyon

“…As shown in Fig. 5, BdCDPK08was significantly upregulated in the seedlings by treatment with all test phytohormones, suggesting BdCDPK08 might play critical roles in phytohormones-induced signaling pathway, and execute multifunction in plant growth and development, which was consistent with the previous reports that the homologs of BdCDPK08 were played important roles in plant growth and development and response to abiotic and biotic stresses (Additional file 14) [32,[79][80][81][82][83]. It has been reported that CDPKs regulated ABA signal pathway by phosphorylating ABA-responsive element binding factors (ABFs) and ABA Insensitive (ABI).…”

“…Lossof-function mutations of CPK4 and CPK11 exhibited pleiotropic ABA-insensitive phenotypes and decreased tolerance of seedlings to salt stress, further study showed that CPK4 and CPK11 positively regulated calciummediated ABA signaling pathways through the phosphorylation of two ABA-responsive transcription factors, ABF1 and ABF4 [28]. Over-expression of OsCDPK7 enhanced plant tolerance to salinity/drought by inducting some stress-responsive genes, but not to cold, while OsCDPK13 and OsCPK17 were supposed to be important signaling components for response to cold stress in rice [30][31][32]. Recent studies have provided compelling evidence for the involvement of CDPKs in immunity signaling and plant disease resistance.…”

The role of calcium-dependent protein kinase in hydrogen peroxide, nitric oxide and ABA-dependent cold acclimation

“…This change in cytoplasmic Ca 2+ is sensed by calcium sensors such as calcium-dependent protein kinases (CPKs), which interact with downstream signaling components including hormones such as abscisic acid (ABA), reactive oxygen species (ROS), and mitogen-activated protein kinases (MPKs), leading to an increased tolerance to various stresses ( Boudsocq and Sheen, 2013 ). CPKs play an important role in signal transduction, and the functions of several CPKs in Arabidopsis and rice have recently been identified ( Almadanim et al , 2017 ; Jin et al , 2017 ). Activated CPKs participate in different signal transduction pathways through the phosphorylation of specific substrates ( Kobayashi et al , 2012 ; Santin et al , 2017 ).…”

SUMOylation of rice DELLA SLR1 modulates transcriptional responses and improves yield under salt stress

“…cv. Nipponbare mature seeds were used for rice transformations according to (Almadanim et al, 2017), and selection for transformants asserted by hygromycin resistance and standard GUS-staining with X-Gluc solution (Roth) of a section of seminal roots. Proteins were extracted, detected and quantified using a modified TCA/Acetone method (Luís et al, 2016).…”