Vaccinia necrosum and its relationship to impaired immunologic responsiveness

Multiple Viral Ligands Naturally Presented by Different Class I Molecules in Transporter Antigen Processing-Deficient Vaccinia Virus-Infected Cells

“…In addition, to identify the HLA restriction of these ligands, new HLApeptide complex stability assays using TAP-deficient T2 cells with specific anti-HLA-B or anti-HLA-C Abs were performed. The numbers of HLA-peptide surface complexes induced by the IAM KRTLLEL (D5R 148-157 ), LPFGSLGI (A50R 294-301 ), and IPSPG IMLV (C11R 101-110 ) synthetic peptides were similar to those induced by a well-known HLA-B51 ligand, HBV HBc [19][20][21][22][23][24][25][26][27] (Fig. 5B), by the use of the anti-HLA-B Ab, indicating that these peptides were restricted by the HLA-B51 class I molecule.…”

“…The A10L 867-876 and K2L [16][17][18][19][20][21][22][23][24][25][26][27][28][29][30] (but not B8R [53][54][55][56][57][58][59] ) peptides have the known anchor motifs for HLA-B*2705 binding of Arg at P2 and basic or aliphatic C-terminal residues (SYFPEITHI database [54]). As the B8R 53-59 peptide was also coimmunoprecipitated with an HLA-B27-specific MAb, it could be an unusual HLA-B27-restricted ligand.…”

“…The Orthopoxvirus vaccinia virus is a widely used tool for research and vaccine development (47), and recent concerns about bioterrorism and emerging infectious diseases have elicited renewed interest in VACV and other poxviruses (31). Vaccination induces a strong humoral response leading to viral clearance, and the role of cellular responses in this crossprotection is well documented (21,56). In recent years, studies using both vaccinated humans and human histocompatibility complex (HLA)-transgenic mouse models have allowed the identification of more than 70 VACV-derived epitopes restricted by various HLA molecules (reviewed in references 30 and 32).…”

“…The following synthetic peptides were used as controls in complex stability assays: KPNA2 (GLVPFLVSV, HLA-A2 restricted) (23), influenza virus NP (SRYWAIRTR, HLA-B27 restricted) (67), hepatitis B virus (HBV) HBc [19][20][21][22][23][24][25][26][27] (LPSDFFPSV, HLA-B51 restricted) (8), cytomegalovirus (CMV) pp65 [7][8][9][10][11][12][13][14][15] (RCPEMISVL, HLA-Cw1 restricted) (33), and C4CON (QYDDAVYLK, HLA-Cw4 restricted) (17). Either RMA-S transfectants or T2 cells expressing small amounts of major histocompatibility complex (MHC) class I on the cell surface were incubated at 26°C for 16 h in RPMI 1640 medium supplemented with 10% heat-inactivated fetal bovine serum (FBS).…”

Role of Metalloproteases in Vaccinia Virus Epitope Processing for Transporter Associated with Antigen Processing (TAP)-independent Human Leukocyte Antigen (HLA)-B7 Class I Antigen Presentation

“…* This work was supported by grants provided by Foundació n para la Inves-Cross-protective vaccination with orthopoxviruses, first with an empirically developed vaccine against cowpox virus and later through the massive worldwide administration of vaccinia virus (VACV), achieved the eradication of smallpox, a pandemic disease caused by variola major virus (10). The role of cellular responses in this cross-protection is well documented (11,12). The cowpox protein CPXV12 inhibits peptide translocation by TAP, thereby interfering with MHC class I-peptide complex formation (13).…”