Vaccines for leishmaniasis in the fore coming 25 years

Palatnik-de-Sousa, Clarisa B.

doi:10.1016/j.vaccine.2008.01.023

Cited by 164 publications

(143 citation statements)

References 156 publications

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“…Finally, the effect of mosquito nets impregnated with insecticide is currently under assessment in India 90 . Excellent reviews regarding the use of insecticides and vaccines for leishmaniasis have been published [91][92][93][94] and should be consulted. Therefore, there are promising efficient alternatives for the control of leishmaniasis besides the traditional measures that widen the horizon of the fight against this disease.…”

Section: Discussionmentioning

confidence: 99%

How effective is dog culling in controlling zoonotic visceral leishmaniasis? a critical evaluation of the science, politics and ethics behind this public health policy

Costa

2011

Rev. Soc. Bras. Med. Trop.

127

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How effective is dog culling in controlling zoonotic visceral leishmaniasis? A critical evaluation of the science, politics and ethics behind this public health policyQuanto é efetivo o abate de cães para o controle do calazar zoonótico? Uma avaliação crítica da ciência, política e ética por trás desta política de saúde pública Carlos Henrique Nery Costa 1,2 ABSTRACT Introduction: Zoonotic kala-azar, a lethal disease caused by protozoa of the genus Leishmania is considered out of control in parts of the world, particularly in Brazil, where transmission has spread to cities throughout most of the territory and mortality presents an increasing trend. Although a highly debatable measure, the Brazilian government regularly culls seropositive dogs to control the disease. Since control is failing, critical analysis concerning the actions focused on the canine reservoir was conducted. Methods: In a review of the literature, a historical perspective focusing mainly on comparisons between the successful Chinese and Soviet strategies and the Brazilian approach is presented. In addition, analyses of the principal studies regarding the role of dogs as risk factors to humans and of the main intervention studies regarding the efficacy of the dog killing strategy were undertaken. Brazilian political reaction to a recently published systematic review that concluded that the dog culling program lacked efficiency and its effect on public policy were also reviewed. Results: No firm evidence of the risk conferred by the presence of dogs to humans was verified; on the contrary, a lack of scientific support for the policy of killing dogs was confirmed. A bias for distorting scientific data towards maintaining the policy of culling animals was observed. Conclusions: Since there is no evidence that dog culling diminishes visceral leishmaniasis transmission, it should be abandoned as a control measure. Ethical considerations have been raised regarding distorting scientific results and the killing of animals despite minimal or absent scientific evidence

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Section: Discussionmentioning

confidence: 99%

How effective is dog culling in controlling zoonotic visceral leishmaniasis? a critical evaluation of the science, politics and ethics behind this public health policy

Costa

2011

Rev. Soc. Bras. Med. Trop.

127

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“…The entire full factorial design of experiment is listed in Table 1. 4 2.5 HSH S 5 2.5 ME S 6 2.5 M+H S 7 4 HSH S 8 4 ME S 9 4 M+H…”

Section: Preparation Of Csln Formulationsmentioning

confidence: 99%

“…Unfortunately, they induce only partial protection and/or need some clinically unacceptable adjuvants to induce proper Th1 response in humans (5). Among them, cysteine proteinases (CPs) are enzymes that belong to the papain super family, which are found in a number of organisms from prokaryotes to mammals.…”

Section: Introductionmentioning

confidence: 99%

Cationic Solid Lipid Nanoparticles Loaded by Cystein Proteinase Genes as a Novel anti-Leishmaniasis DNA Vaccine Delivery System: Characterization and in vitro Evaluations

et al. 2010

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-Purpose. Leishmaniasis is a major health problem in many tropical and sub-tropical countries and development of a safe and easily-available vaccine has high priority. Although several antigens potentially capable of inducing protective immunity have been studied, in the absence of pharmaceutical industry interest they have remained as fine publications only. Amongst them, Cathepsin L-like cysteine proteinases (CPs) have received considerable attention and type I and II CPs have been used in a heterologous prime-boost vaccination regime for experimental visceral leishmaniasis in dogs. Due to the promising results of the mentioned vaccination regime, we aimed to evaluate cationic solid lipid nanoparticles (cSLNs) for in vitro delivery of cpa, cpb and cpb CTE intended to be used as a cocktail DNA vaccine in our forthcoming studies. Methods: cSLNs were formulated of cetyl palmitate, cholesterol, DOTAP and Tween 80 via melt emulsification method followed by high shear homogenization. Different formulations were prepared by anchoring pDNAs on the surface of cSLNs via charge interaction. The formulations were characterized according to their size and zeta potential as well as pDNA integrity and stability against DNase I treatment. Lipoplexes' cytotoxicity was investigated on COS-7 cells by MTT test. The effect of the DOTAP:pDNA ratio on protection ability and cytotoxicity was also studied. In vitro transfection efficiency was qualified by florescent microscopy and quantified using flow cytometry technique. Results: cSLN-pDNA complexes were formulated with suitable size and zeta potential. Efficiency/cytotoxicity ratio of cSLN-pDNAs formulations was comparable to linear PEI-25kDa-pDNAs polyplexes while exhibiting significantly lower cytotoxicity. Conclusion: Tested formulations were able to deliver immunogenic CP genes efficiently. This data proves the ability of this system as a promising DNA vaccine carrier for leishmaniasis to cover the main drawback of naked pDNA delivery that is rapidly elimination from the circulation.

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“…Information about a multitude of immunization approaches representing all of the major vaccine design strategies, including vaccines using live genetically attenuated parasites, subunit proteins/peptides in adjuvants, naked DNA and infectious vectored vaccines expressing genes coding for specific leishmanial antigens and combinations thereof has been given in recent review articles (Coler & Reed 2005, Tabbara 2006, Kedzierski et al 2006, Palatnik-de-Souza 2008, Silvestre et al 2008). Many of these vaccines have been tested for immunogenicity and protective efficacy in a variety of experimental models (such as inbred laboratory rodents, dogs and NHP).…”

Section: Vaccine Studies Against Leishmaniasismentioning

confidence: 99%

“…Although host genetics can have dramatic effects on T-cell responses to existing vaccines (Lambert et al 2005), several technical problems (including inadequate information about the quality, stability and potency of the antigens) may provide explanation for some of the variation in efficacy observed in those human vaccine studies. Nevertheless, most experts believe that a preventive vaccine will be essential if this disease is ever to be controlled worldwide (Coler & Reed 2005, Tabbara 2006, Kedzierski et al 2006, Palatnik-de-Souza 2008, Silvestre et al 2008.…”

mentioning

confidence: 99%

The utility of rhesus monkey (Macaca mulatta) and other non-human primate models for preclinical testing of Leishmania candidate vaccines

Grimaldi

2008

Mem. Inst. Oswaldo Cruz

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Vaccines for leishmaniasis in the fore coming 25 years

Cited by 164 publications

References 156 publications

How effective is dog culling in controlling zoonotic visceral leishmaniasis? a critical evaluation of the science, politics and ethics behind this public health policy

How effective is dog culling in controlling zoonotic visceral leishmaniasis? a critical evaluation of the science, politics and ethics behind this public health policy

Cationic Solid Lipid Nanoparticles Loaded by Cystein Proteinase Genes as a Novel anti-Leishmaniasis DNA Vaccine Delivery System: Characterization and in vitro Evaluations

The utility of rhesus monkey (Macaca mulatta) and other non-human primate models for preclinical testing of Leishmania candidate vaccines

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