Vaccination with a live multi-gene deletion strain protects horses against virulent challenge with Streptococcus equi

Robinson, Carl; Heather, Zoe; Slater, Josh; Potts, Nicola; Steward, Karen F.; Maskell, Duncan J.; Fontaine, Michaël C.; Lee, Jeongjin; Smith, Ken; Waller, Andrew S.

doi:10.1016/j.vaccine.2015.01.019

Cited by 14 publications

(16 citation statements)

References 29 publications

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“…Five of the nine vaccinated ponies developed injection site abscesses caused by the vaccine strain where two were removed from the study. Six out of nine control ponies were pyrexic following challenge whereas none of the seven vaccinated ponies were significantly febrile (Robinson et al 2015). Nine out of nine control ponies had lymph node abscesses, whereas only one of the seven vaccinated ponies had a lymph node abscess (Robinson et al 2015).…”

Section: S Equi Vaccines Under Investigationmentioning

confidence: 88%

“…An intramuscular multigene deletion strain, where sagA, hasA, seM, aroB, pyrC, and recA genes were deleted, was investigated (Robinson et al 2015). Deletion of sagA, hasA, and seM genes was performed to reduce ability to resist phagocytosis and improve vaccine safety.…”

Section: S Equi Vaccines Under Investigationmentioning

confidence: 99%

“…Deletion of sagA caused loss of production of the hemolytic toxin, streptolysin S (SLS) making the colonies non-hemolytic as well as making the colonies less able to colonize tracheal mucosa (Robinson et al 2015). Deletion of hasA resulted in non-encapsulated colonies susceptible to phagocytosis.…”

Section: S Equi Vaccines Under Investigationmentioning

confidence: 99%

“…Deletion of hasA resulted in non-encapsulated colonies susceptible to phagocytosis. Deletion of aroB and pyrC reduced the ability to produce aromatic amino acids and pyrimidines; and deletion of recA reduced the environmental persistence by increasing the susceptibility to ultraviolet light (Robinson et al 2015). Nine ponies were vaccinated intramuscularly on Day 0 and seven ponies were revaccinated on Day 56.…”

Section: S Equi Vaccines Under Investigationmentioning

confidence: 99%

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Comparison of Immunologic Responses Following Intranasal and Oral Administration of a USDA-Approved, Live-Attenuated Streptococcus equi Vaccine

Delph

Davis

Bello

et al. 2018

Journal of Equine Veterinary Science

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Section: S Equi Vaccines Under Investigationmentioning

confidence: 88%

Section: S Equi Vaccines Under Investigationmentioning

confidence: 99%

Section: S Equi Vaccines Under Investigationmentioning

confidence: 99%

Section: S Equi Vaccines Under Investigationmentioning

confidence: 99%

See 2 more Smart Citations

Comparison of Immunologic Responses Following Intranasal and Oral Administration of a USDA-Approved, Live-Attenuated Streptococcus equi Vaccine

Delph

Davis

Bello

et al. 2018

Journal of Equine Veterinary Science

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“…equi (strain 4047) and mutant strains were as described (Paillot et al, 2010;Robinson et al, 2015; equi (strain 4047) and mutant strains were as described (Paillot et al, 2010;Robinson et al, 2015; …”

Section: Bacteriamentioning

confidence: 99%

StreptococcalsagAactivates a proinflammatory response in mast cells by a sublytic mechanism

Beek

Waern

Eriksson

et al. 2019

Cellular Microbiology

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Mast cells are implicated in the innate proinflammatory immune defence against bacterial insult, but the mechanisms through which mast cells respond to bacterial encounter are poorly defined. Here, we addressed this issue and show that mast cells respond vividly to wild type Streptococcus equi by up‐regulating a panel of proinflammatory genes and by secreting proinflammatory cytokines. However, this response was completely abrogated when the bacteria lacked expression of sagA, whereas the lack of a range of other potential virulence genes (seeH, seeI, seeL, seeM, hasA, seM, aroB, pyrC, and recA) had no effect on the amplitude of the mast cell responses. The sagA gene encodes streptolysin S, a lytic toxin, and we next showed that the wild type strain but not a sagA‐deficient mutant induced lysis of mast cells. To investigate whether host cell membrane perturbation per se could play a role in the activation of the proinflammatory response, we evaluated the effects of detergent‐ and pneumolysin‐dependent lysis on mast cells. Indeed, exposure of mast cells to sublytic concentrations of all these agents resulted in cytokine responses of similar amplitudes as those caused by wild type streptococci. This suggests that sublytic membrane perturbation is sufficient to trigger full‐blown proinflammatory signalling in mast cells. Subsequent analysis showed that the p38 and Erk1/2 signalling pathways had central roles in the proinflammatory response of mast cells challenged by either sagA‐expressing streptococci or detergent. Altogether, these findings suggest that sagA‐dependent mast cell membrane perturbation is a mechanism capable of activating the innate immune response upon bacterial challenge.

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Equine vaccines

Tizard¹

2021

Vaccines for Veterinarians

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Vaccination with a live multi-gene deletion strain protects horses against virulent challenge with Streptococcus equi

Cited by 14 publications

References 29 publications

Comparison of Immunologic Responses Following Intranasal and Oral Administration of a USDA-Approved, Live-Attenuated Streptococcus equi Vaccine

Comparison of Immunologic Responses Following Intranasal and Oral Administration of a USDA-Approved, Live-Attenuated Streptococcus equi Vaccine

StreptococcalsagAactivates a proinflammatory response in mast cells by a sublytic mechanism

Equine vaccines

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