Vaccination of Young Calves Against Trichophytosis

Rybnikář, A.; Vrzal, V.; Chumela, J.

doi:10.2754/avb199362010055

Cited by 11 publications

(9 citation statements)

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“…Trichophyton verrucosum infectious culture was applied to calves aged between 1 day and 4 months in our experiments. Both the rise of the experimental infection and its extent were not dependent on the age of the experimental animals; this finding complies with the previously published results 10 . The rise of the first clinical trichophytic changes was not affected significantly by the extent of skin damage; it obviously depends also on the individual sensitivity of animals to dermatophytic infection.…”

Section: Discussionsupporting

confidence: 91%

Experimental dermatophytosis in calves caused by Trichophyton verrucosum culture

Obořilová

Rybnikář

2005

Mycoses

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Microconidia suspension of Trichophyton verrucosum virulent culture isolated originally from the mycotic lesions of cattle was inoculated epicutaneously to calves in various doses. Four application methods were selected, namely, onto the sheared and gently scarified skin, onto the sheared and non-scarified skin, onto the non-sheared and gently scarified skin, and onto the non-sheared and non-scarified skin. The inoculated area was 10 x 10 cm for all cases mentioned. When the application was performed onto the sheared and scarified skin, at least 18,000 microconidia of T. verrucosum per one animal had to be used to induce the mycotic disease in 100% of animals. This value was 10 times higher when the application was performed onto the non-sheared and scarified skin, 100 times higher in the case of the sheared and non-scarified skin and 1,000 times higher in the case of the non-sheared and non-scarified skin. The minimum infectious dose still capable of inducing dermatophytosis was 180 microconidia per 100 cm(2) of the scarified skin, 18,000 microconidia per 100 cm(2) of the sheared and non-scarified skin, and 180,000 microconidia per 100 cm(2) of the non-sheared and non-scarified skin.

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Section: Discussionsupporting

confidence: 91%

Experimental dermatophytosis in calves caused by Trichophyton verrucosum culture

Obořilová

Rybnikář

2005

Mycoses

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“…sporadically (Rybnikáfi et al 1991;1993). In this regard the stage of pregnancy of the cows must be considered, because, generally, weaker immune responses in pregnant females cannot be ruled out.…”

Section: Discussionmentioning

confidence: 99%

Immunisation of Pregnant Cows Against Ringworm and its Effect on the Progeny

Rybnikář¹,

Chumela²,

Vrzal³

2001

Acta Vet. Brno

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The topic of the study was the development of immunity and its possible transmission to progeny in pregnant cows vaccinated against ringworm. Twelve experimental pregnant cows were vaccinated twice against ringworm and another twelve cows served as non-vaccinated controls. Calves born by the experimental and the control cows were challenged epicutaneously with a virulent strain of Trichophyton verrucosum. The experimental cows were challenged with the same agent ten months after the second vaccination. The challenge resulted in mycotic lesions that were less severe and healed sooner in the vaccinated than in the control cows. No difference in the clinical course of ringworm induced by challenge were observed between the calves born by the experimental and the control cows. It is concluded that pregnant cows vaccinated against ringworm developed immunity which was not transmitted to their progeny. The vaccination had no effect on pregnancy, parturition and the state of health of newborn calves. The results were not processed by statistical methods. Immunisation, Trichophyton verrucosum, pregnant cowsThe effects of vaccination of late pregnant cows against ringworm using the Russian vaccines TF-130 and LTF-130 on the state of health of the vaccinates and newborn calves and on the course of pregnancy were studied by Jilavjan et al. (1976). However, the authors did not investigate the development of immunity and its possible transfer to the progeny. Materials and MethodsOur experiment was conducted in Holstein cows in a herd that had been free of ringworm for the last ten years. The cows had never been vaccinated against ringworm.Twelve cows aged two to eight years were vaccinated intramuscularly in the gluteal region with 5 ml of a live commercial vaccine against ringworm (Trichoben, Bioveta, Ivanovice na Hané, Czech Republic); six of them were vaccinated on pregnancy day 113 to 190 and the remaining six on pregnancy day 191 to 212. The vaccination with the same dose in the opposite gluteal region was repeated after twelve days.Another twelve cows served as non-vaccinated controls. All the cows were monitored for the course of pregnancy and parturition and for the state of health of newborn calves. The calves were left with their dams for two days to assure sufficient intake of colostrum. All calves born by the vaccinated and the control cows were challenged epicutaneously with a virulent strain of T. verrucosum at the age of 26 to 72 days. The challenge dose of 5 × 10 6 CFU was rubbed into a 10 × 10 cm area of shaved and scarified epidermis on the right side.Both the vaccinated and the control cows were challenged in the same way ten months after the second vaccination.The development of skin lesions at the challenge site in the calves and cows was monitored for 43 and 34 days, respectively. Samples of flakes, crusts or hair for inoculation onto Sabouraud agar supplemented with chloramphenicol and actidion were collected from the lesions towards the end of the observation period. ResultsNo undesirable local or gen...

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“…One month after the last vaccination all calves including the non-vaccinated controls (group 8; n = 13) were challenged using the method described by Rybnikář et al (1993). The challenge dose of Trichophyton -without clinical skin trichophytic changes ± minute skin changes (scales, papulae) + solitary trichophytic lesions ++ merging trichophytic lesions covering less than 50% of the inoculated area +++ merging trichophytic lesions covering more than 50% of the inoculated area Table 1 verrucosum CCM F-650 virulent culture isolated originally from cattle with clinical mycotic changes was 1 million of CFU per animal in this experiment.…”

Section: Methodsmentioning

confidence: 99%

Efficacy Test of Trichoben Vaccine Administered to Calves at Different Intervals between Vaccination and Re-Vaccination

Rybnikář¹,

Obořilová²,

Hedbávný³

2008

Acta Vet. Brno

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The aim of the study was to test a possible extension of the period between vaccination and revaccination with the live Trichoben vaccine (Bioveta, a.s., Czech Republic). The calves (n = 61) were vaccinated intramuscularly. The intervals between vaccination and re-vaccination were 3, 5, 7, 10, 14 or 28 days. Another group of calves (n = 16) was vaccinated once with a double dose of the vaccine. The protective immunity against experimental infection with a virulent Trichophyton verrucosum CCM F-650 strain was investigated in animals one month after the last vaccination and compared with a group of non-vaccinated controls (n = 16). Clinical results were evaluated and compared using balanced two-way-ANOVA. The level of post-vaccination immunity in cattle after re-vaccination was sufficient when the intervals were 5, 7, 10, 14 and 28 days. One-time injection of a double prophylactic dose of the vaccine and a 3-day interval between vaccinations reduced the immunity and vaccine efficacy to an unsatisfactory degree. There were significant differences between the groups of calves vaccinated using an interval longer than 5 days, and the group vaccinated once with a double dose, and the group with a 3-day interval between vaccinations. The level of post-vaccination immunity in cattle after re-vaccination at the commonly used interval of 10 -14 days was identical with the immunity achieved when the interval was reduced to 5 or 7 days or extended up to 28 days. The extension of the interval between vaccinations to 5 -28 days is recommended for using the Trichoben vaccine in veterinary practice.

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Vaccination of Young Calves Against Trichophytosis

Cited by 11 publications

References 1 publication

Experimental dermatophytosis in calves caused by Trichophyton verrucosum culture

Experimental dermatophytosis in calves caused by Trichophyton verrucosum culture

Immunisation of Pregnant Cows Against Ringworm and its Effect on the Progeny

Efficacy Test of Trichoben Vaccine Administered to Calves at Different Intervals between Vaccination and Re-Vaccination

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