Vaccination of squirrel monkeys (Saimiri spp.) with nanoparticle-based Toxoplasma gondii antigens: new hope for captive susceptible species

Ducournau, Céline; Cantin, Pauline; Alerte, Vanessa; Quintard, Benoît; Popelin-Wedlarski, Florine; Wedlarski, Rudy; Ollivet-Courtois, Florence; Maltot, Johanne Ferri-Pisani; Herkt, Clément; Fasquelle, François; Sannier, Mylène; Berthet, Mélanie; Fretay, Vanessa; Aubert, Dominique; Villena, Isabelle; Betbeder, Didier; Moiré, Nathalie; Dimier‐Poisson, Isabelle

doi:10.1016/j.ijpara.2023.02.003

Cited by 2 publications

(8 citation statements)

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“…Based on these results, the vaccination of captive Saimiris was proposed in 6 French zoos dealing with T. gondii infection ( Ducournau et al, 2023 ). The vaccine was considered as an experimental prophylactic procedure which, in the absence of an alternative, could help in tackling a real health issue for these captive animals.…”

Section: Discussionmentioning

confidence: 99%

“…After 5 years of survey, no death related to infection by T. gondii was observed, while 3–26% of animals used to die each year from this infection. A Th1 immune response was induced by the vaccination, without any humoral response ( Ducournau et al, 2023 ).…”

Section: Discussionmentioning

confidence: 99%

“…The titration of anti- T. gondii serum antibodies was assessed by ELISA before the beginning of the study and after each of the 3 immunizations ( Ducournau et al, 2023 ). A 96-well plate was coated with 100 μL per well of 10 μg/ml of inactivated T. gondii , in carbonate buffer at pH 9.6, overnight at 4 °C.…”

Section: Methodsmentioning

confidence: 99%

“…Recently, a prophylactic vaccine was tested in French zoos where lethal T. gondii infection occurred annually ( Ducournau et al, 2023 ). The vaccine was based on inactivated T. gondii , associated with lipidated maltodextrine-based nanoparticles (NPL), and was administered twice nasally at one-month intervals, followed by heterologous nasal/subcutaneous boosts 6 months, one year and two years after the beginning of the study.…”

Section: Introductionmentioning

confidence: 99%

“…The vaccine was based on inactivated T. gondii , associated with lipidated maltodextrine-based nanoparticles (NPL), and was administered twice nasally at one-month intervals, followed by heterologous nasal/subcutaneous boosts 6 months, one year and two years after the beginning of the study. No death has occurred among vaccinated animals in the participating zoos since the first administration, thanks to a memory Th1 immune response ( Ducournau et al, 2023 ).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Nasal vaccination of six squirrel monkeys (Saimiri sciureus): Improved immunization protocol against Toxoplasma gondii with a nanoparticle-born vaccine

Fasquelle,

Scuotto,

Vreulx

et al. 2023

International Journal for Parasitology: Parasites and Wildlife

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Nasal vaccination of six squirrel monkeys (Saimiri sciureus): Improved immunization protocol against Toxoplasma gondii with a nanoparticle-born vaccine

Fasquelle,

Scuotto,

Vreulx

et al. 2023

International Journal for Parasitology: Parasites and Wildlife

Self Cite

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Human T-cell activation withToxoplasma gondiiantigens loaded in maltodextrin nanoparticles

García-López,

Zamora-Vélez,

Vargas-Montes

et al. 2024

Life Sci. Alliance

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Toxoplasmosis is the most prevalent parasitic zoonosis worldwide, causing ocular and neurological diseases. No vaccine has been approved for human use. We evaluated the response of peripheral blood mononuclear cells (PBMCs) to a novel construct ofToxoplasma gondiitotal antigen in maltodextrin nanoparticles (NP/TE) in individuals with varying infectious statuses (uninfected, chronic asymptomatic, or ocular toxoplasmosis). We analyzed the concentration of IFN-γ after NP/TE ex vivo stimulation using ELISA and the immunophenotypes of CD4+and CD8+cell populations using flow cytometry. In addition, serotyping of individuals with toxoplasmosis was performed by ELISA using GRA6-derived polypeptides. Low doses of NP/TE stimulation (0.9 μg NP/0.3 μg TE) achieved IFN-γ–specific production in previously exposed human PBMCs without significant differences in the infecting serotype. Increased IFN-γ expression in CD4+effector memory cell subsets was found in patients with ocular toxoplasmosis with NP/TE but not with TE alone. This is the first study to show how T-cell subsets respond to ex vivo stimulation with a vaccine candidate for human toxoplasmosis, providing crucial insights for future clinical trials.

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Vaccination of squirrel monkeys (Saimiri spp.) with nanoparticle-based Toxoplasma gondii antigens: new hope for captive susceptible species

Cited by 2 publications

References 81 publications

Nasal vaccination of six squirrel monkeys (Saimiri sciureus): Improved immunization protocol against Toxoplasma gondii with a nanoparticle-born vaccine

Nasal vaccination of six squirrel monkeys (Saimiri sciureus): Improved immunization protocol against Toxoplasma gondii with a nanoparticle-born vaccine

Human T-cell activation withToxoplasma gondiiantigens loaded in maltodextrin nanoparticles

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