Vaccination of mice with liposome-entrapped adult antigens of Nippostrongylus brasiliensis

Rhalem, Abdelkbir; Bourdieu, Christiane; Luffau, G.; Pery, P.

doi:10.1016/0769-2625(88)90037-2

Cited by 16 publications

(6 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Promising results have also been obtained following immunization with liposome-associated antigens; the oral (3,19,35,37,47) and parenteral (4,8,13,24,25,38,39,42) routes have been used for antigens of parasites (4,24,25,37,39), viruses (13,32), and bacteria (3,8,19,35,38,42,47). Their potential as adjuvants has been demonstrated in several studies, in which the use of liposome-associated antigens resulted in protective immunity (4,8,19,24,25,37,39,42) or at least cell-mediated (8) and humoral responses (3,13,38). The adjuvanticity of liposomes seems to depend on several factors including vesicle size and structure, lipid constitution, surface charge, antigen localization, the animal species immunized, route of immunization, and the distribution and number of lamellae.…”

mentioning

confidence: 99%

Antibody responses in the serum and respiratory tract of mice following oral vaccination with liposomes coated with filamentous hemagglutinin and pertussis toxoid

et al. 1993

View full text Add to dashboard Cite

Mice were orally vaccinated with liposomes coated with filamentous hemagglutinin (FHA) and detoxified pertussis toxin (PT) of BordeteUla pertussis. FHA-and PT-specific immunoglobulin G (IgG) was detected in serum, and both IgG and IgA were detected in lung washes following the immunization. Antibody responses in mice immunized with liposomes coated with FHA and PT were significantly higher than those in mice immunized with free FHA and PT, which demonstrated the adjuvanticity of the liposome carrier. The results indicate the potential usefulness of this approach for eliciting immune responses against FHA and PT (and perhaps other pertussis antigens) in humans and its possible utility in large-scale vaccination to protect against both B. pertussis infection and disease.

show abstract

mentioning

confidence: 99%

Antibody responses in the serum and respiratory tract of mice following oral vaccination with liposomes coated with filamentous hemagglutinin and pertussis toxoid

et al. 1993

View full text Add to dashboard Cite

show abstract

“…Thus far, the oral administration of liposomes has been undertaken for antigens of parasite [25] and bacteria [5,7,8,10,12,21,24,29]. Their potential as adjuvants has been demonstrated in several studies [21,25]. However, the critical point in the oral use of liposomes is the stability of the vesicles in the harsh environment of the gastrointestinal tract.…”

mentioning

confidence: 99%

“…In particular, since the liposomesentrapped materials are protected from enzymatic attack until they reach the target sites, the potential usefulness of liposomes as carriers and adjuvants for developing oral vaccines has attracted considerable interest during the last few years [1]. Thus far, the oral administration of liposomes has been undertaken for antigens of parasite [25] and bacteria [5,7,8,10,12,21,24,29]. Their potential as adjuvants has been demonstrated in several studies [21,25].…”

mentioning

confidence: 99%

Antibody Response in the Intestinal Tract of Mice Orally Immunized with Antigen Associated with Liposomes.

Watarai

Han

Tana

et al. 1998

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. In order to evaluate the usefulness of liposomes, which are stable in acidic solution, bile and pancreatin solution (stable liposomes), as vehicle for oral vaccines, the intestinal IgA antibody responses of mice to liposome-associated antigen after oral administration were examined. The intestinal IgA antibody responses against ganglioside GM1 were detected after the oral immunization of ganglioside GM1-containing stable liposomes. When monophosphoryl lipid A was incorporated into stable liposomes containing ganglioside GM1, further augmentation of IgA responses to ganglioside GM1 was observed. On the other hand, the oral administration with ganglioside GM1 alone was unable to induce any detectable intestinal anti-ganglioside GM1 IgA antibody response. These results suggest that liposomes which are stable in acidic solution, bile, and pancreatin solution would serve effectively as an oral delivery vehicle for inducing mucosal immune responses. -KEY WORDS: liposome, mucosal immunity, oral vaccine.

show abstract

“…Thus far, the oral administration of liposomes has been undertaken for antigens of parasite [23] and bacteria [5, 7-9, 11, 19, 22, 29]. Their potential as adjuvants has been demonstrated in several studies, in which the use of liposome-associated antigens resulted in protective immunity [9,23]. However, there have been few reports that when liposomes are used as a carrier of orally administered antigens to enhance the mucosal immune response, they are delivered to Peyer's patches without degradation or release of entrapped antigens during passage through the gastrointestinal tract [3].…”

mentioning

confidence: 99%

“…Recently, it was shown that liposomes were taken up by rat Peyer's patches following intraluminal and oral administration [6,13,26], suggesting that liposomes have potential for developing oral vaccines. Thus far, the oral administration of liposomes has been undertaken for antigens of parasite [23] and bacteria [5, 7-9, 11, 19, 22, 29]. Their potential as adjuvants has been demonstrated in several studies, in which the use of liposome-associated antigens resulted in protective immunity [9,23].…”

mentioning

confidence: 99%

Application of Liposomes for Development of Oral Vaccines: Study of In Vitro Stability of Liposomes and Antibody Response to Antigen Associated with Liposomes after Oral Immunization.

Han

Watarai

Kobayashi

et al. 1997

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. In order to evaluate the usefulness of liposomes as oral vaccines, the stability of liposomes and serum IgA antibody response to antigen associated with liposomes after oral administration were examined. Liposomes composed of dipalmitoylphosphatidylcholine (DPPC), dipalmitoylphosphatidylserine (DPPS), and cholesterol (Chol) (1:1:2, molar ratio), distearoylphosphatidylcholine (DSPC) and Chol (7:2, molar ratio), and DSPC, DPPS, and Chol (7:3:2 or 1:1:2, molar ratio) were stable in acidic solution (pH 2.0), bile, and pancreatin solution, whereas liposomes composed of DPPC and Chol (7:2, molar ratio) and DPPC, DPPS, and Chol (7:3:2, molar ratio) were unstable in pH 2.0 and/or bile solutions. After the oral immunization of antigen (ganglioside GM1)-containing liposomes composed of DPPC, DPPS, and Chol (1:1:2, molar ratio) to mice, the serum IgA antibody responses against ganglioside GM1 were found. Furthermore, when monophosphoryl lipid A was incorporated into liposomes containing ganglioside GM1, further augmentation of IgA responses to ganglioside GM1 was observed. On the other hand, the oral administration with liposomes composed of DPPC, Chol, and ganglioside GM1 (unstable liposomes), ganglioside GM1 mixed with liposomes composed of DPPC, DPPS and Chol, and ganglioside GM1 alone was unable to induce any detectable anti-ganglioside GM1 IgA antibody responses. These results suggest that liposomes which showed the stability to acidic solution, bile, and pancreatin solution would serve effectively as an oral delivery vehicle for inducing mucosal immune responses. -KEY WORDS: liposome, mucosal immunity, oral administration, oral vaccine, stability.

show abstract

Vaccination of mice with liposome-entrapped adult antigens of Nippostrongylus brasiliensis

Cited by 16 publications

References 13 publications

Antibody responses in the serum and respiratory tract of mice following oral vaccination with liposomes coated with filamentous hemagglutinin and pertussis toxoid

Antibody responses in the serum and respiratory tract of mice following oral vaccination with liposomes coated with filamentous hemagglutinin and pertussis toxoid

Antibody Response in the Intestinal Tract of Mice Orally Immunized with Antigen Associated with Liposomes.

Application of Liposomes for Development of Oral Vaccines: Study of In Vitro Stability of Liposomes and Antibody Response to Antigen Associated with Liposomes after Oral Immunization.

Contact Info

Product

Resources

About