Antibody responses in the serum and respiratory tract of mice following oral vaccination with liposomes coated with filamentous hemagglutinin and pertussis toxoid

Abstract

Mice were orally vaccinated with liposomes coated with filamentous hemagglutinin (FHA) and detoxified pertussis toxin (PT) of BordeteUla pertussis. FHA-and PT-specific immunoglobulin G (IgG) was detected in serum, and both IgG and IgA were detected in lung washes following the immunization. Antibody responses in mice immunized with liposomes coated with FHA and PT were significantly higher than those in mice immunized with free FHA and PT, which demonstrated the adjuvanticity of the liposome carrier. The results indicate the potential usefulness of this approach for eliciting immune responses against FHA and PT (and perhaps other pertussis antigens) in humans and its possible utility in large-scale vaccination to protect against both B. pertussis infection and disease.

Novel polymer‐grafted starch microparticles for mucosal delivery of vaccines

“…7 Particulate antigen delivery methods offer an approach to enhancing the immunogenicity of orally administered soluble immunogens. Such delivery systems include liposomes, [8][9][10] immune-stimulating complexes (ISCOMs), 11,12 protein cochleates 13 and biodegradable microparticles (MP) constructed, for example, of poly(lactide-co-glycolide) (PLG), [14][15][16][17][18][19] thermally condensed polyaminoacids, 20 polyphosphazenes, 21 albumin, 22,23 and derivitized starch. 24 Notably, MP can enhance and prolong mucosal and systemic antibody responses following oral immunization.…”

Polymer‐grafted starch microparticles for oral and nasal immunization

“…In contrast, particulate antigen delivery methods can enhance the immunogenicity of mucosally delivered soluble immunogens. These delivery systems include liposomes, 7 , 8 immunostimulating complexes (ISCOM), 9 protein cochleates, 10 proteosomes 11 and microparticles (MP) fabricated from a variety of materials (Table 1). 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 Microparticles enhance and prolong mucosal and systemic antibody responses after oral or nasal immunization, most likely by temporarily protecting antigen from the acidic and enzymatically hostile environments found at mucosal surfaces, facilitating MP uptake by M cells overlying Peyer's patches (PP) 30 , 31 , 32 and/or mucosal epithelium, 33 thereby promoting antigen transport to local lymphoid follicles and associated lymph nodes, and probably by establishing depots of antigen locally 30 .…”