Vaccination of chickens with DNA vaccine expressing Eimeria tenella MZ5-7 against coccidiosis

Assessment of humoral immunity to Eimeria tenella sporozoites in chickens by ELISA

“…However, there was no significant difference in the mean lesion score of all groups administered with the sporozoites (P>0.05). This finding is in agreement with previous reports [16,17].…”

Detection of Cell Mediated Immune Response in Chickens Immunized with Eimeria tenella Sporozoites

“…A low mean lesion score was observed in T4 (2.5±0.22) followed by T2 (2.83±0.17), T1 (3.33±0.33) and T3 (3.00±0.45) in comparison with unimmunised infected T5 (3.33±0.33) thus indicating a partial protection (P>0.05) by the sporozoites. A similar observation was recorded by Ziomko et al, (2005) and Geriletu et al, (2011). Interferons have been reported to be inimical to parasites, probably because of their ability to inhibit parasite development (Lillihoj and Choi, 1998), promote production of free radicals (Dimier and Bout, 1997) and activate antibody-dependent cell-mediated cytotoxicity (Fleischer, 1980).…”

Efficacy of chimeric DNA vaccines encoding Eimeria tenella 5401 and chicken IFN-γ or IL-2 against coccidiosis in chickens

“…The cytokine IL-2 is a potent growth factor for a variety of cell types including involvement in T-cell differentiation, B-cell development and NK-cell activation. The protective effect of IL-2 on vaccination of chickens with coccidia genes was recently demonstrated by DNA vaccination (Dalloul and Lillehoj, 2006;Geriletu et al, 2011;Song et al, 2013). In this study, chicken IFN-γ gene and IL-2 gene were fused with 5401 gene to construct the chimeric DNA vaccine pVAX-5401-IFN-γ and pVAX-5401-IL-2, respectively.…”

“…A fourth and even fifth dose would be given unless the titers of the antiserum were satisfactory. The antiserum was collected and stored at −20°C for further use (Geriletu et al, 2011).…”

“…The rat antiserum against recombinant 5401 protein was prepared as described in Section 2.1 (Geriletu et al, 2011). The antiserum was collected and stored at −20°C for the subsequent western blot assay.…”