Efficacy of chimeric DNA vaccines encoding Eimeria tenella 5401 and chicken IFN-γ or IL-2 against coccidiosis in chickens

Song, Xiaoling; Huang, Xinmei; Ren, Yan; Xu, Lixin; Li, Xiangrui

doi:10.1016/j.exppara.2015.05.003

Cited by 27 publications

(21 citation statements)

References 27 publications

(50 reference statements)

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“…IL-2 mRNA transcripts level in the spleen and intestine was significantly enhanced after infections with E. acervulina . IL-2 has been demonstrated to be able to significantly improve the protective effect of recombinant coccidia genes by DNA vaccination [ 29 , 34 ]. In this study, the mRNA transcripts level of IFN-γ and IL-2 were significantly increased by vaccination with pVAX-Ea14–3-3 (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Although the Ea14–3-3 DNA vaccine provided effective protection against Eimeria , some measures could be taken to improve protection and to make it more practical for use in fields [ 42 ]. The protective efficacy of the DNA vaccine could be enhanced by co-injection with plasmids encoding immune stimulating cytokines (IFN-γ, IL-2) [ 29 , 34 , 43 ]. The sugarcane ( Saccharum officinarum L.) bagasse-derived polysaccharides could be used as native immunomodulatory candidate to improve the protective efficacy of the DNA vaccine [ 44 ].…”

Section: Discussionmentioning

confidence: 99%

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Protective immunity induced by Eimeria common antigen 14–3-3 against Eimeria tenella, Eimeria acervulina and Eimeria maxima

Liu

et al. 2018

BMC Vet Res

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BackgroundAvian coccidiosis is often caused by co-infection with several species of Eimeria worldwide. Developing a multivalent vaccine with an antigen common to multiple Eimeria species is a promising strategy for controlling clinical common co-infection of Eimeria. In the previous study, 14–3-3 was identified as one of the immunogenic common antigen in E. tenella, E. acervulina and E. maxima. The aim of the present study was to evaluate the immunogenicity and protective efficacy of Ea14–3-3 in the form of DNA vaccine against infection with three species of Eimeria both individually and simultaneously.ResultsAfter vaccination with pVAX-Ea14–3-3, the Ea14–3-3 gene was transcribed and expressed in the injected muscles. Vaccination with pVAX-Ea14–3-3 significantly increased the proportion of CD4+ and CD8+ T lymphocytes and produced a strong IgY response in immunized chickens. Similarly, pVAX-Ea14–3-3 stimulated the chicken’s splenocytes to produce high levels of Th1-type (IFN-γ, IL-2) and Th2-type (IL-4) cytokines. The vaccine-induced immune response was responsible to increase weight gain, decreased the oocyst output, and alleviated enteric lesions significantly in immunized chickens as compared to control group, in addition to induce moderate anti-coccidial index (ACI).ConclusionThese results indicate that Ea14–3-3 is highly immunogenic and capable to induce significant immune responses. Furthermore, Ea14–3-3 antigen can provide effective protection against infection with Eimeria tenella, Eimeria acervulina, Eimeria maxima both individually and in combination with three Eimeria species. Significant outcomes of our study provide an effective candidate antigen for developing a multivalent Eimeria vaccine against mixed infection with various Eimeria species under natural conditions.Electronic supplementary materialThe online version of this article (10.1186/s12917-018-1665-z) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Protective immunity induced by Eimeria common antigen 14–3-3 against Eimeria tenella, Eimeria acervulina and Eimeria maxima

Liu

et al. 2018

BMC Vet Res

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“…For example, immune responses induced by DNA vaccination can be enhanced by co-injection with recombinant cytokines or plasmids encoding these cytokines (Min et al, 2002; Song et al, 2015b). The efficacy of DNA vaccination also could be increased by optimization of vaccination procedure including route, dose, time of immunization and age of primary immunization of chickens (Song et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…The membrane was incubated with anti- E. acervulina and anti- E. maxima chicken sera (1: 100) and horseradish peroxidase (HRP)-conjugated Goat anti-chicken IgG (1:2000, Sigma–Aldrich) as secondary antibody. The bound antibody was detected using 3, 30-diaminobenzidine (DAB) (Song et al, 2015b). …”

Section: Methodsmentioning

confidence: 99%

Protective Efficacy of Coccidial Common Antigen Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) against Challenge with Three Eimeria Species

Tian

Huang

et al. 2017

Front. Microbiol.

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Coccidiosis is an intestinal disorder of poultry and often caused by simultaneous infections of several Eimeria species. GAPDH is one of the immunogenic common antigens among Eimeria tenella, E. acervulina, and E. maxima identified in our previous study. The present study was performed to further evaluate its immunogenicity and protective efficacy. The genes of GAPDH cloned from E. acervulina and E. maxima were named as EaGAPDH and EmGAPDH, respectively. The immunogenicity of recombinant proteins of EaGAPDH and EmGAPDH were analyzed by Western blot. The transcription and expression of pVAX-EaGAPDH and pVAX-EmGAPDH in the injected muscles were detected by reverse transcription PCR (RT-PCR) and Western blot, respectively. GAPDH-induced changes of T lymphocytes subpopulation, cytokines production, and antibody were determined using flow cytometry, quantitative real-time PCR (qPCR), and ELISA, respectively. Finally, the protective efficacies of pVAX-EaGAPDH and pVAX-EmGAPDH were evaluated by vaccination and challenge experiments. The results revealed that the recombinant GAPDH proteins reacted with the corresponding chicken antisera. The EaGAPDH genes were successfully transcribed and expressed in the injected muscles. Vaccination with pVAX-EaGAPDH and pVAX-EmGAPDH significantly increased the proportion of CD4+ and CD8+ T lymphocytes, the cytokines productions of IFN-γ, IL-2, IL-4 et al., and IgG antibody levels compared to controls. The vaccination increased the weight gains, decreased the oocyst outputs, alleviate the enteric lesions compared to controls, and induced moderate anti-coccidial index (ACI). In conclusion, the coccidial common antigen of GAPDH induced significant humoral and cellular immune response and effective protection against E. tenella, E. acervulina, E. maxima, and mixed infection of the three Eimeria species.

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“…Several studies have confirmed that the recombinant EtMIC2 protein shows good immunogenicity and might be a good candidate for use in vaccine development (Ding et al 2005 ; Sathish et al 2011 ; Zhang et al 2014 ). To enhance its anti-coccidial effect, several cytokines were inserted into eukaryotic expression plasmids in tandem with antigen genes to immunize the host (Geriletu et al 2011 ; Song et al 2013 ; Song et al 2015 ; Song et al 2016 ). In recent years, studies have revealed that the cytokine chIL-18 could enhance the protective efficacy of immunization and host immune responses (Shi et al 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Immunological evaluation of invasive Lactobacillus plantarum co-expressing EtMIC2 and chicken interleukin-18 against Eimeria tenella

Zhang¹,

Huang²,

Jiang³

et al. 2020

Parasitol Res

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Chicken coccidiosis is a protozoan parasitic disease that leads to considerable economic losses in the poultry industry. In this study, we used invasive Lactobacillus plantarum ( L.P ) expressing the FnBPA protein as a novel bacterial carrier for DNA delivery into epithelial cells to develop a live oral DNA vaccine. A fusion DNA vaccine co-expressing EtMIC2 and chicken IL-18 (chIL-18) was constructed and then delivered to the host by invasive L.P . Its efficacy against Eimeria tenella challenge was evaluated in chickens by examining the relative weight gain rate; caecal lesion score; OPG; anti-coccidial index (ACI); levels of EtMIC2 antibody, FnBPA, IL-4, IL-18, IFN-γ and SIgA; and proliferation ability and percentages of CD4 + and CD8 + splenocytes. The experimental results showed that chickens immunized with invasive L.P carrying the eukaryotic expression vector pValac-EtMIC2 (pValac-EtMIC2/pSIP409-FnBPA) had markedly improved immune protection against challenge compared with that of chickens immunized with non-invasive L.P (pValac-EtMIC2/pSIP409). However, invasive L.P co-expressing EtMIC2 with the chIL-18 vector exhibited the highest protection efficiency against E. tenella . These results indicate that invasive Lactobacillus -expressing FnBPA improved humoural and cellular immunity and enhanced resistance to E. tenella . The DNA vaccine delivered by invasive Lactobacillus provides a new concept and method for the prevention of E. tenella .

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Efficacy of chimeric DNA vaccines encoding Eimeria tenella 5401 and chicken IFN-γ or IL-2 against coccidiosis in chickens

Cited by 27 publications

References 27 publications

Protective immunity induced by Eimeria common antigen 14–3-3 against Eimeria tenella, Eimeria acervulina and Eimeria maxima

Protective immunity induced by Eimeria common antigen 14–3-3 against Eimeria tenella, Eimeria acervulina and Eimeria maxima

Protective Efficacy of Coccidial Common Antigen Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) against Challenge with Three Eimeria Species

Immunological evaluation of invasive Lactobacillus plantarum co-expressing EtMIC2 and chicken interleukin-18 against Eimeria tenella

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