Protective Efficacy of Coccidial Common Antigen Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) against Challenge with Three Eimeria Species

Tian, Lili; Li, Wenyu; Huang, Xinmei; Tian, Di; Liu, Jianhua; Yang, XinChao; Liu, Lianrui; Ren, Yan; Xu, Lixin; Li, Xiangrui; Song, Xiaoling

doi:10.3389/fmicb.2017.01245

Cited by 34 publications

(39 citation statements)

References 42 publications

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“…The presence of upregulated immunogenic PP2C suggests that PP2C is a potential vaccine candidate for toxoplasmosis [40]. As a common antigen of Eimeria, GAPDH could induce strong humoral and cellular immune responses, and had effective protection against E. tenella, E. acervulina and E. maxima as a potential vaccine candidate [41]. In our present study, GAPDH as an immunodominant antigen was confirmed to be immunologically shared by all three Eimeria species.…”

Section: Discussionsupporting

confidence: 68%

Immunoproteomic and mass spectrometric analysis of Eimeria acervulina antigens recognized by antisera from chickens infected with E. acervulina, E. tenella or E. necatrix

Liu

Tuo

et al. 2020

Parasites Vectors

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Background: Coccidiosis is caused by Eimeria spp. and can result in severe economic losses to the global poultry industry. Due to anticoccidial drug resistance rapidly developing in the parasites and drug residues in poultry products, efficacious and safe alternative coccidia control measures are needed. The objective of the present study was to identify common protective antigens which may be used as vaccine candidates in the development of subunit, multivalent, cross-protective vaccines against most of the economically important Eimeria species.Methods: Whole sporozoite proteins of Eimeria acervulina were prepared and analyzed by 2-dimensional gel electrophoresis (2-DE) followed by western blotting using immune sera specific to E. tenella, E. acervulina, or E. necatrix. The protein spots detected by all three immune sera were then excised from the preparative gel and protein ID was performed by MALDI-TOF-MS/MS. Results:Approximately 620 E. acervulina sporozoite protein spots were demonstrated by 2-DE with silver staining, among which 23 protein spots were recognized by immune sera specific to all three Eimeria species. The results showed that 21 putative E. acervulina proteins were identified, which include proteins with known enzymatic properties, and those which are involved in protein translation, transport and trafficking, and ribosomal biogenesis and functions. There is one protein which may be involved in transcription and one heat-shock protein. Two proteins contain predicted domains, but with no apparent functions known. There were 2 protein spots which had no detectable proteins. None of the proteins has a predicted signal peptide or a transmembrane domain; however, 6 of the 21 putative proteins were predicted to be potentially secretory through the non-classical pathway. Conclusions:Our study identified a diverse group of antigens immunologically common to all three Eimeria species, none of which was previously characterized and tested as a vaccine candidate. Further research on immunogenicity and cross-protective potential of these individual proteins as vaccine candidates will aid the development of vaccines against the most common and pathogenic Eimeria spp.

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Section: Discussionsupporting

confidence: 68%

Immunoproteomic and mass spectrometric analysis of Eimeria acervulina antigens recognized by antisera from chickens infected with E. acervulina, E. tenella or E. necatrix

Liu

Tuo

et al. 2020

Parasites Vectors

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“…In this case, CD8α was significantly downregulated in the intestine of Ross 308 broilers at 6d pi with both infectious doses and CD4 expression was upregulated significantly less, at 13d pi, compared to Ranger Classic broilers. A study by Hong et al reported that E. maxima infection increased both CD8 and CD4 expression at 6‐8 days pi and both CD4 and CD8 lymphocytes numbers, which may have occurred via recognition of antigens such as those encoded by E. maxima surface antigen genes or Eimeria glyceraldehyde‐3‐phosphate dehydrogenase . However, the genotype of these chickens was not reported but the result probably reflects the use of a different genotype to the ones we have used and further highlights the importance of genetic background when extrapolating data between different studies of Eimeria infection in chickens.…”

Section: Discussionmentioning

confidence: 79%

Differential immune response to Eimeria maxima infection in fast‐ and slow‐growing broiler genotypes

Giles

Sakkas

Belkhiri

et al. 2019

Parasite Immunology

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Very little has been reported comparing resistance to coccidiosis in fast or slow growing broilers, the latter of which are becoming more prevalent in the broiler industry. We examined mRNA expression in the intestines of fast and slow growing broilers following Eimeria infection. We show that by day 13 post‐infection (d pi) with 2500 or 7000 oocysts of Eimeria maxima, slower‐growing (Ranger Classic) broilers significantly (P < 0.01) upregulated expression of proinflammatory cyclooxygenase genes (LTB4DH, PTSG1 and PTSG2) above that detected in fast growing (Ross 308) broilers. Expression of CD8α mRNA was downregulated in Ross 308 at day 6d pi with either 2500 or 7000 oocysts of E maxima (P < 0.05), compared to uninfected controls, but was not differentially expressed in Ranger Classic. CD4 genes were not differentially expressed in either chicken line infected with either infectious oocyst dose at d6 pi, compared to uninfected controls. However, at d13 pi, CD4 expression was significantly upregulated in both chicken lines infected with either infectious oocyst dose, compared to uninfected controls (P < 0.05) but this was significantly greater in Ranger Classic broilers compared to Ross 308 (P < 0.05). At d13 pi, expression of CD3 chains (required for T lymphocyte activation) was significantly increased in Ranger Classic compared to Ross 308, infected with either oocyst dose (P < 0.05‐0.01). Expression of IL‐2 and IL‐15 mRNA, required for T lymphocyte proliferation was also significantly upregulated, or maintained longer, in Ranger Classic broilers compared to Ross 308. These differences in immune response to E maxima corresponded with a reduction in E maxima genome detected in the intestines of Ranger Classic compared to Ross 308.

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“…Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed to detect the puri ed rEmROM2. The rat anti-rEmROM2 serum was prepared by the protocol previously described [8] for the detection of Western blot. Meanwhile, the serum from noninjected rat was used as a negative control.…”

Section: Preparation Of Remrom2 and Anti-remrom2 Serummentioning

confidence: 99%

“…Chicken anti-E. maxima serum was obtained by the method previously described [8,20]. A Western blot assay was carried out with the above chicken antiserum as primary antibody (serum from uninfected chicken was set as negative control), and horseradish peroxidase (HRP)-conjugated goat anti-chicken IgG (Sigma-Aldrich, Darmstadt, Germany) as secondary antibody.…”

Section: Western Blot Recognition Of Remrom2 By Chicken Anti-e Maximmentioning

confidence: 99%

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Rhomboid Protein 2 of Eimeria Maxima Provided Partial Protection Against Infection by Homologous Species

Chen

Tian

Chen

et al. 2020

Preprint

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Background: Rhomboid-like proteases (ROMs) are considered as a new candidate antigen for developing new-generation vaccine due to their important role involved in the invasion of apicomplexan protozoa. In prior works, we obtained a ROM2 sequence of Eimeria maxima (EmROM2) which is the homologous gene with ROM2 of Toxoplasma gondii. This study was conducted to evaluate the immunogenicity and protective efficacy of EmROM2 recombinant protein (rEmROM2) and EmROM2 DNA (pVAX1-EmROM2) against infection by Eimeria maxima (E. maxima).Methods: Western blot assay was conducted to analyze the immunogenicity of rEmROM2. Reverse transcription-polymerase chain reaction (RT-PCR) and Western blot assay were performed to determine the transcription and expression of pVAX1-EmROM2 recombinant plasmid. EmROM2-induced changes in transcriptional level of cytokines, T lymphocytes subsets and specific serum IgG antibody were detected through qPCR (quantitative real-time PCR), flow cytometry and indirect ELISA, respectively. Ultimately, a vaccination-challenge trial was performed to evaluate the protective efficacy of rEmROM2 and pVAX1-EmROM2 against infection with E. maxima. Results: The purified rEmROM2 was recognized with chicken anti-E. maxima serum. After vaccination with pVAX1-EmROM2, apparent transcription and translation of EmROM2 were observed in the vaccinated chickens. Vaccination with rEmROM2 and EmROM2 DNA significantly upregulated the proportion of CD8+ and CD4+ T lymphocytes, the transcription level of cytokines (IFN-γ, IL-2, IL-4, IL-10, IL-17, TGF-β and TNF SF15) and serum IgG antibody response. Meanwhile, the vaccination significantly alleviated enteric lesions, weight loss, and reduced oocyst output caused by challenge infection of E. maxima, and provided anticoccidial index (ACI) of more than 160, indicating partial protection against E. maxima.Conclusions: Vaccination with rEmROM2 and pVAX1-EmROM2 activated notable humoral and cell-mediated immunity and provided partial protection against infection by E. maxima. These results demonstrated that EmROM2 protein and DNA are promising vaccine candidates against E. maxima infection.

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Protective Efficacy of Coccidial Common Antigen Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) against Challenge with Three Eimeria Species

Cited by 34 publications

References 42 publications

Immunoproteomic and mass spectrometric analysis of Eimeria acervulina antigens recognized by antisera from chickens infected with E. acervulina, E. tenella or E. necatrix

Immunoproteomic and mass spectrometric analysis of Eimeria acervulina antigens recognized by antisera from chickens infected with E. acervulina, E. tenella or E. necatrix

Differential immune response to Eimeria maxima infection in fast‐ and slow‐growing broiler genotypes

Rhomboid Protein 2 of Eimeria Maxima Provided Partial Protection Against Infection by Homologous Species

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