Vaccination of chicken embryos with escape mutants of La Sota Newcastle disease virus induces a protective immune response

Mast, Jan; Nanbru, C.; Decaesstecker, Mireille; Lambrecht, Bénédicte; Couvreur, Bernard; Meulemans, G.; Berg, Thierry van den

doi:10.1016/j.vaccine.2005.10.020

Cited by 39 publications

(27 citation statements)

References 31 publications

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“…This supports the idea that the immune function of broiler chickens during the late embryonic and the neonatal stage is not entirely developed due to the incomplete structural organization of their secondary immune organs (8). This is in contrast with the active immunity that can be obtained, although delayed, after in ovo or day-old vaccination with a live vaccine or vectorized antigens that have the ability to induce new germinal centers and mature a specific immune response (7,9).…”

Section: Discussionsupporting

confidence: 74%

Passive Protection Afforded by Maternally-Derived Antibodies in Chickens and the Antibodies' Interference with the Protection Elicited by Avian Influenza–Inactivated Vaccines in Progeny

Vriese¹,

Steensels²,

Palya³

et al. 2010

Avian Diseases

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Systematic vaccination can be applied when a disease has become enzootic in a country or region. The final goal of the approach is to control or eradicate the disease within the country. This is a long-term vaccination plan that could be applied nationwide to all commercial and backyard poultry. However, after several months of vaccination in enzootic areas, maternally derived antibody (MDA) is present in young chicks, providing some protection and/or interference with vaccination. The aim of this study was to evaluate the level of protection afforded by MDA against challenge with highly pathogenic avian influenza virus (HPAIV), and its suspected interference with current inactivated vaccines in broilers under controlled laboratory conditions. In the first set of experiments, broilers were vaccinated with inactivated vaccines containing H5N2 subtype antigens in the presence or absence of homologue MDAs and challenged with a clade 2.2 H5N1 HPAIV. In the second set of experiments, day-old broilers, either with or without avian influenza MDA, received a regular-type monovalent H5N2 AI vaccine (0.5 ml) or a concentrated (0.2 ml) AL-Newcastle disease virus combined inactivated vaccine subcutaneously. They were then challenged at 11 or 35 days of age. In conclusion, our results indicate that protection induced by day-old administration of inactivated vaccine (regular or concentrated) in the presence or absence of MDA to H5N2 AIV induces poor protection against challenge with H5N1 HPAIV and should not be recommended. Based on our results, vaccination of MDA-positive chickens at a later age (10 days) seems to be a valuable recommendation, although MDAs may still interfere with vaccination to a lesser extent because they are present up to 3 wk posthatch. Therefore, in areas with high infection pressure, when possible, two vaccinations are recommended for optimal protection. Also, it might be advisable to take into account day-old AI MDA titers when one is determining the optimal age of vaccination.

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Section: Discussionsupporting

confidence: 74%

Passive Protection Afforded by Maternally-Derived Antibodies in Chickens and the Antibodies' Interference with the Protection Elicited by Avian Influenza–Inactivated Vaccines in Progeny

Vriese¹,

Steensels²,

Palya³

et al. 2010

Avian Diseases

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“…When a sufficient number of strains are compared, the higher the NS/S ratio is, the greater the role of a selective pressure [10]. In recent years, our laboratory, along with other research groups, have studied the influence of the immune selective pressure on the evolution of chicken leukemia virus [7], NDV attenuated vaccine [15], and H9N2 subtype avian influenza virus [8] in cell culture or embryonated eggs. As a continuation of the above work, this paper focused on the evolution of the NDV TZ060107 field strain during passages in CEF with NDV-specific antibodies.…”

Section: Discussionmentioning

confidence: 99%

Epitope variation in the Newcastle disease virus HN gene under antibody immune selective pressure in cell culture

Gong

Cui

2011

Sci. China Life Sci.

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The influence of antibody immune selective pressure on Newcastle disease virus (NDV) HN and F gene mutations was studied in cell cultures. NDV field strain TZ060107 was inoculated into chicken embryo fibroblast cells and continuously passaged with (group A) or without (group B) anti-NDV monospecific serum. Each group contained three independent passage series. HN and F genes were amplified and sequenced for the 10th, 20th, 30th, 40th and 50th generations of each serial passage, and compared with the original strain. The results demonstrated that increased HN gene mutations were observed in group A with the antibody than in group B without the antibody. The nonsynonymous (NS) to synonymous (S) mutations ratio was 6 for group A, significantly higher than 3.4 in group B. In group A with the antibody, there were five stable NS mutations in HN gene, three of which (related to aa#353, 521 and 568) were related to known epitopes. There were two stable NS mutations in F gene in group A, but no stable NS mutations in group B. The NS/S ratios of F gene were less than 2.5 for both groups A and B. Our results suggested that the antibody strongly influenced HN gene mutations, while the F gene was less influenced by the same antibody.

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“…But the use of a chemical reagent is not preferable, and the V protein-defective recombinant virus could not induce an antibody response sufficient to overcome maternal antibodies (1,34). Recently, several escape mutants of La Sota were evaluated as in ovo vaccine candidates, but their embryonic and neonatal lethality rates were still high (32). On the other hand, KBNP-C4152R2L displayed several outstanding properties as an embryo vaccine candidate.…”

Section: Fig 2 Comparison Of Efficacies Of the Killed Kbnp-c4152r2lmentioning

confidence: 99%

Characterization of a Recombinant Newcastle Disease Virus Vaccine Strain

Cho

Kwon

Kim

et al. 2008

Clin Vaccine Immunol

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A recombinant La Sota strain (KBNP-C4152R2L) in which fusion (F) and hemagglutinin-neuraminidase (HN) genes were replaced with those of a contemporary genotype VIId virus, KBNP-4152, has been developed. To attenuate the virulence of the recombinant strain, the F cleavage motif was mutated from 112 RRQKR 116 to 112 GRQAR 116 , and to reduce pathogenic instability, a codon which does not allow changes to basic amino acids by single point mutation was inserted at codon 115. In addition a six-nucleotide sequence was inserted into the intergenic region between matrix protein and F genes for attenuation without breaking the "rule-of-six." The HN protein length was increased from 571 to 577 as a marker. Serological tests revealed that the antigenicity of KBNP-C4152R2L was similar to that of KBNP-4152 but distinct from that of the La Sota strain. KBNP-C4152R2L was avirulent (intracerebral pathogenicity index, 0.0; mean death time, >168 h) and stable in pathogenicity through in vivo passages. The killed oil emulsion of and live KBNP-C4152R2L were completely protective against mortality and egg drop caused by virulent strains, and KBNP-C4152R2L was applicable to in ovo vaccination. Therefore, KBNP-C4152R2L is a promising vaccine strain and viral vector in terms of antigenicity, productivity, safety, and pathogenic stability.

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Vaccination of chicken embryos with escape mutants of La Sota Newcastle disease virus induces a protective immune response

Cited by 39 publications

References 31 publications

Passive Protection Afforded by Maternally-Derived Antibodies in Chickens and the Antibodies' Interference with the Protection Elicited by Avian Influenza–Inactivated Vaccines in Progeny

Passive Protection Afforded by Maternally-Derived Antibodies in Chickens and the Antibodies' Interference with the Protection Elicited by Avian Influenza–Inactivated Vaccines in Progeny

Epitope variation in the Newcastle disease virus HN gene under antibody immune selective pressure in cell culture

Characterization of a Recombinant Newcastle Disease Virus Vaccine Strain

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