Vaccination of cattle against tropical theileriosis in Uzbekistan using autochthonous live vaccine

Rasulov, I.; Fish, L.; Shkap, Varda

doi:10.1016/j.vaccine.2008.10.001

Cited by 7 publications

(2 citation statements)

References 15 publications

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“…Vaccination, with infected cell lines that develop attenuated virulence upon long-term culture, has been utilised in several countries [2, 3]. These vaccines can provide protection against clinical disease in the field but do not prevent establishment of carrier status.…”

Section: Introductionmentioning

confidence: 99%

Identification of candidate transmission-blocking antigen genes in Theileria annulata and related vector-borne apicomplexan parasites

et al. 2017

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BackgroundVector-borne apicomplexan parasites are a major cause of mortality and morbidity to humans and livestock globally. The most important disease syndromes caused by these parasites are malaria, babesiosis and theileriosis. Strategies for control often target parasite stages in the mammalian host that cause disease, but this can result in reservoir infections that promote pathogen transmission and generate economic loss. Optimal control strategies should protect against clinical disease, block transmission and be applicable across related genera of parasites. We have used bioinformatics and transcriptomics to screen for transmission-blocking candidate antigens in the tick-borne apicomplexan parasite, Theileria annulata.ResultsA number of candidate antigen genes were identified which encoded amino acid domains that are conserved across vector-borne Apicomplexa (Babesia, Plasmodium and Theileria), including the Pfs48/45 6-cys domain and a novel cysteine-rich domain. Expression profiling confirmed that selected candidate genes are expressed by life cycle stages within infected ticks. Additionally, putative B cell epitopes were identified in the T. annulata gene sequences encoding the 6-cys and cysteine rich domains, in a gene encoding a putative papain-family cysteine peptidase, with similarity to the Plasmodium SERA family, and the gene encoding the T. annulata major merozoite/piroplasm surface antigen, Tams1.ConclusionsCandidate genes were identified that encode proteins with similarity to known transmission blocking candidates in related parasites, while one is a novel candidate conserved across vector-borne apicomplexans and has a potential role in the sexual phase of the life cycle. The results indicate that a ‘One Health’ approach could be utilised to develop a transmission-blocking strategy effective against vector-borne apicomplexan parasites of animals and humans.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-3788-1) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Identification of candidate transmission-blocking antigen genes in Theileria annulata and related vector-borne apicomplexan parasites

et al. 2017

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“…Anaplasma marginale and Babesia bigemina have been reported serologically in cattle in Tajikistan [ 26 ]. Live vaccines against infections caused by T. annulata and B. bigemina have been developed and applied in Uzbekistan [ 27 , 28 ]. There is no information about T. orientalis genotypes in Kazakhstan, Tajisiktan, and Uzbekistan located on the border of Kyrgyzstan.…”

Section: Discussionmentioning

confidence: 99%

Detection of Theileria orientalis Genotypes from Cattle in Kyrgyzstan

et al. 2022

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The ikeda and chitose genotypes of Theileria orientalis, which for many years were thought to be benign, cause a disease that results in significant economic losses in the cattle industry. This study was carried out in order to determine the genotypes of T. orientalis in cattle in Kyrgyzstan, and 149 archived DNA samples known to be T. orientalis were analyzed by the PCR amplification of the major piroplasm surface protein (MPSP) gene region. Single-Strand Conformation Polymorphism (SSCP) analysis was performed to uncover the nucleotide changes in the archived DNA samples, and 15 samples showing different band profiles were subjected to sequence analysis. As a result of the sequence analysis, it was seen that the samples belonged to the buffeli and chitose A genotypes. In order to identify mixed genotypes, PCR was performed using primers specific for these genotypes, and buffeli (type 3), chitose (type 1) and buffeli+chitose were found to be positive in 26.2%, 2% and 71.8% of samples, respectively. As a result of this study, we showed the presence of buffeli (type 3) and chitose (type 1) genotypes of T. orientalis in cattle in Kyrgyzstan. Comprehensive epidemiological studies are needed to understand the clinical infections caused by the pathogenic chitose A and to determine the geographical distribution and different genotypes of T. orientalis.

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