First molecular survey of piroplasm species in cattle from Kyrgyzstan

Aktaş, Münir; Kısadere, İhsan; Özübek, Sezayi; Cihan, Hüseyin; Salikov, Ruslan; Çirak, Veli Yılgör

doi:10.1007/s00436-019-06370-2

Cited by 14 publications

(14 citation statements)

References 21 publications

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“…In addition, T. luwenshuni infection in ticks from dogs and cattle was identified by reverse line blot hybridization (34). Aktaş et al (38) revealed the presence of T. orientalis and T. annulata from cattle in Kyrgyzstan, which is near Bole. In south India, which is near Yecheng and Tashikurgan, researchers also identified T. orientalis in ticks (29).…”

Section: Resultsmentioning

confidence: 99%

Molecular Detection and Identification of Babesia spp., Theileria spp., and Anaplasma spp. in Sheep From Border Regions, Northwestern China

Galon

Guo

et al. 2020

Front. Vet. Sci.

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Babesia, Theileria, and Anaplasma are important causative agents of tick-borne diseases that severely affect sheep. However, there is paucity in the occurrence genetic diversity of the infections of tick-borne diseases in sheep in border regions, northwestern China. In this study, nested polymerase chain reaction (nPCR) assays and gene sequencing were used to identify tick-borne Babesia spp., Theileria spp., and Anaplasma spp. infections in border regions, northwestern China. Out of 323 samples tested in this study, 225 (69.7%) sheep were infected with Babesia spp., Theileria spp., and Anaplasma spp. Two hundred six (63.8%), 60 (18.6%), 54 (16.7%), 51 (15.8%), 32 (9.9%), 19 (5.9%), and 16 (5.0%) were positive for A. ovis, B. motasi-like, A. bovis, T. uilenbergi, A. phagocytophilum, T. luwenshuni, and B. motasi-like Xinjiang, respectively. The most common dual infection was with A. ovis and B. motasi-like while the most frequent triple coinfection was A. ovis, B. motasi-like, and T. uilenbergi with coinfection rates of 17.0% (55/323) and 5.0% (16/323), respectively. Sequencing analysis indicated that A. ovis MSP4, A. phagocytophilum epank1, A. bovis 16S rRNA, B. motasi-like rap1-b, B. motasi-like Xinjiang rap1-a, T. luwenshuni 18S rRNA, and T. uilenbergi 18S rRNA from border regions, northwestern China, showed 99-100% identity with documented isolates from other countries. To the best of the authors' knowledge, this is the first report of T. uilenbergi and T. luwenshuni infections of sheep in border regions, northwestern China. Furthermore, these findings provide important data for understanding the distribution of Babesia, Theileria, and Anaplasma in sheep between border countries and China.

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Section: Resultsmentioning

confidence: 99%

Molecular Detection and Identification of Babesia spp., Theileria spp., and Anaplasma spp. in Sheep From Border Regions, Northwestern China

Galon

Guo

et al. 2020

Front. Vet. Sci.

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“…The detection rate of T. orientalis was not negligible either, as 4 out of 13 sequenced specimens tested positive. The frequent occurrence of this species has mostly been associated with cattle from Asia [28,38]. This Theileria species do not harbor high pathogenicity for cattle.…”

Section: Discussionmentioning

confidence: 99%

Bovines Harbor a Diverse Array of Vector-Borne Pathogens in Northeast Algeria

et al. 2020

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Arthropod-borne hemoparasites represent a serious health problem in livestock, causing significant production losses. Currently, the evidence of Anaplasma spp., Theileria spp., Babesia spp., and hemotropic Mycoplasma spp. in Algeria remains limited to a few scattered geographical regions. In this work, our objectives were to study the prevalence of these vector-borne pathogens and to search other agents not yet described in Algeria as well as the identification of statistical associations with various risk factors in cattle in the northeast of Algeria. Among the 205 cattle blood samples tested by PCR analysis, 42.4% positive results were obtained for at least one pathogen. The overall rates of Anaplasma spp., Theileria/Babesia spp., and Mycoplasma spp. in the cattle sampled were respectively 30.7%, 18.5%, and 2.9%; co-infections with multiple species was also detected. Anaplasma spp. and Theileria/Babesia spp. were detected at a higher rate in cattle under 3 years old, according to univariate analysis. Anaplasma spp. DNA was detected more frequently in our sample in cattle living in semi extensive farming. Our study provides additional data about Anaplasma spp., Theileria/Babesia spp. and reveals for the first time that Mycoplasma wenyonii and ‘Candidatus Mycoplasma hemobos are present in cattle in Northeast Algeria.

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“…Archived DNA samples used in this study and detailed information from the investigated provinces has been documented previously [ 11 ]. Briefly, this study was carried out between December 2012 and June 2013 on 454 cattle in eight provinces (Karaşar, Kayyngdy, Kızıl-Töbö, Kopuro Bazar, Moldovanovka, Sokuluk, Tamga, and Tokmok) located around the Chu valley and Issyk Kul Lake in Kyrgyzstan ( Figure 1 ).…”

Section: Methodsmentioning

confidence: 99%

“…Briefly, this study was carried out between December 2012 and June 2013 on 454 cattle in eight provinces (Karaşar, Kayyngdy, Kızıl-Töbö, Kopuro Bazar, Moldovanovka, Sokuluk, Tamga, and Tokmok) located around the Chu valley and Issyk Kul Lake in Kyrgyzstan ( Figure 1 ). All DNA samples were tested using the reverse line blot assay for bovine Theileria and Babesia species to determine the frequency of piroplasm distribution, and 149 DNA samples identified as containing T. orientalis [ 11 ] were used for genotype analysis.…”

Section: Methodsmentioning

confidence: 99%

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Detection of Theileria orientalis Genotypes from Cattle in Kyrgyzstan

et al. 2022

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The ikeda and chitose genotypes of Theileria orientalis, which for many years were thought to be benign, cause a disease that results in significant economic losses in the cattle industry. This study was carried out in order to determine the genotypes of T. orientalis in cattle in Kyrgyzstan, and 149 archived DNA samples known to be T. orientalis were analyzed by the PCR amplification of the major piroplasm surface protein (MPSP) gene region. Single-Strand Conformation Polymorphism (SSCP) analysis was performed to uncover the nucleotide changes in the archived DNA samples, and 15 samples showing different band profiles were subjected to sequence analysis. As a result of the sequence analysis, it was seen that the samples belonged to the buffeli and chitose A genotypes. In order to identify mixed genotypes, PCR was performed using primers specific for these genotypes, and buffeli (type 3), chitose (type 1) and buffeli+chitose were found to be positive in 26.2%, 2% and 71.8% of samples, respectively. As a result of this study, we showed the presence of buffeli (type 3) and chitose (type 1) genotypes of T. orientalis in cattle in Kyrgyzstan. Comprehensive epidemiological studies are needed to understand the clinical infections caused by the pathogenic chitose A and to determine the geographical distribution and different genotypes of T. orientalis.

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First molecular survey of piroplasm species in cattle from Kyrgyzstan

Cited by 14 publications

References 21 publications

Molecular Detection and Identification of Babesia spp., Theileria spp., and Anaplasma spp. in Sheep From Border Regions, Northwestern China

Molecular Detection and Identification of Babesia spp., Theileria spp., and Anaplasma spp. in Sheep From Border Regions, Northwestern China

Bovines Harbor a Diverse Array of Vector-Borne Pathogens in Northeast Algeria

Detection of Theileria orientalis Genotypes from Cattle in Kyrgyzstan

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