Untitled

Nieves, Nadina; Zambrano, Yumaris; Tapia, R.; Cid, Mariela; Pina, Danilo; Castillo, R

doi:10.1023/a:1025855611981

Cited by 25 publications

(6 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Also, encapsulation of propagules that were produced in vitro could reduce the cost of micropropagation of plantlets for commercialization and final delivery (Chu 1995, Nieves et al 2003. This technology may be of value in breeding programs and allows the propagation of many elite genotypes in a short time (Nieves et al 1998).…”

Section: Introductionmentioning

confidence: 99%

In vitro regeneration protocol for artificial seed production in an important medicinal plant Mentha arvensis L

Islam

Bari

2014

J bio-sci.

View full text Add to dashboard Cite

Context: The application of encapsulated shoot tips and nodal segments may contribute to the protection of rare and threatened medicinal plants. Although the artificial seed technique has been reported for more than two decades, for medicinal plants this method has not been developed sufficiently. The main limitations in conventional propagation of some species with medicinal value are: reduced endosperm, low germination rate and seedless varieties. The above mentioned reasons indicate the need for the production of artificial seeds as a technique which combines the advantages of clonal multiplication with those of seed propagation and storage. Objectives: The objective of the present investigation was to standardize artificial seed production technology taking shoot tip and nodal explants in Mentha arvensis and its in vitro regeneration Materials and Methods: Sodium alginate beads were produced by encapsulation of shoot tip and nodal segments of the plant M. arvensis. MS medium was used as basal medium with agar and sodium alginate was used as gelling agent accompanied by CaCl2 solution. Results: Different concentrations and combinations of BAP, Kin and NAA were used in alginate bead in MS basal medium. Among the different concentrations of phytohormone, highest 80% of shoot formation was observed in MS medium containing 2.0 mg/l BAP + 0.2 mg/l NAA from nodal segments of M. arvensis. Highest average number of shoot 9.87 ± 0.58 formation was obtained in the same medium but highest length of shoot 6.27 ± 0.29 cm was found in the medium having 1.0 mg/l BAP + 0.5 mg/l NAA. Conclusion:The present investigation clearly established and demonstrated the method of obtaining the artificial seed production in M. arvensis supported by different hormone concentrations

show abstract

Section: Introductionmentioning

confidence: 99%

In vitro regeneration protocol for artificial seed production in an important medicinal plant Mentha arvensis L

Islam

Bari

2014

J bio-sci.

View full text Add to dashboard Cite

show abstract

“…Nyende, Schittenhelm, Mix-Wagner, and Greef (2005) report that in vitro precultivation of synthetic potato seeds is necessary for success in the field because direct sowing in the field had less than 18% emergence. No difference in sugar yield was found after 12 months of field cultivation when synthetic seed-acclimatized sugarcane plants were compared to macropropagated plants derived from pregerminated three-bud propagules and from single-node auxiliary buds (Nieves et al, 2003). These results demonstrate that precultivation is an alternative for the production of seedlings with good field performance and that nutrient solutions added to the growth medium stimulate the initial plant growth.…”

Section: Discussionmentioning

confidence: 80%

Sugarcane Bud Chip Encapsulation for ex vitro Synthetic Seed Formation

Silva¹,

Neto²,

Severiano³

et al. 2018

JAS

View full text Add to dashboard Cite

Altough the sugarcane crop had a huge world importance the planting system stil the same since the development, needing changes to increase the procution potential. So, the objective of this study was to assess the effect of sugarcane bud chip encapsulation on the initial growth of seedlings. To provide informations for a new planting system, using small stem pieces of sugarcane to produce the seedlings. Two experiments were conducted in a completely randomized design. In the first, bud chip encapsulation was assessed with six concentrations of sodium alginate (0, 10, 20, 30, 40, and 50 g L -1 ) cross-linked with 300 mM calcium chloride, with the encapsulated chips being kept in a greenhouse. In the second experiment, the capsules resulting from the different sodium alginate concentrations were tested for the dry mass adhered to the bud chip, moisture, swelling index, biodegradability, and solubility. Emergence greater than 70% was obtained at sodium alginate concentrations of 0, 10, and 20 g L -1. The 30, 40, and 50 g L -1 concentrations inhibited seedling emergence and initial growth; however, when the capsule was removed, the bud chips formed viable seedlings. Encapsulation inhibited emergence because the capsule acts as a physical barrier; however, encapsulation may be used for bud chip preservation. The study of new capsules and encapsulation methods may enable the ex vitro production of synthetic sugarcane seeds.

show abstract

“…Por otro lado, el encapsulado de tejidos como segmentos nodales de plantas con importancia frutícola (Rai et al, 2009) se ha aplicado en cítricos (Nieves et al, 1998) en plantas medicinales como Centella asiatica (Prasad et al, 2014), y en alfalfa (Medicago sativa L.) (Fujii et al, 1992). En caña de azúcar (Saccharum officinarum L.) variedad CP-5243, los embriones encapsulados se han plantado directamente en campo (Nieves et al, 2003).…”

Section: Introductionunclassified

Optimización de las condiciones de producción de cápsulas con microestacas para aumentar el tiempo de almacenamiento de la planta medicinal mexicana Castilleja tenuiflora (Orobanchaceae)

et al. 2019

View full text Add to dashboard Cite

Antecedentes y Objetivos: Castilleja tenuiflora es una de las plantas mexicanas, silvestres y medicinales cultivadas in vitro que presentan actividad biológica antiinflamatoria y antiulcerogénica. La encapsulación de microestacas es una opción para propagar y prolongar el almacenamiento de esta planta. El objetivo de este estudio fue establecer un método para la encapsulación y conservación de microestacas de C. tenuiflora sin afectar el contenido de sus compuestos.Métodos: Las cápsulas fueron elaboradas con alginato de sodio en complejo con cloruro de calcio dihidratado como matriz y microestacas de tejido proveniente de plántulas cultivadas in vitro. Se probaron diferentes concentraciones y tiempos de polimerización, así como diferentes tiempos de almacenamiento y temperatura, para posteriormente analizar los metabolitos mayoritarios de los extractos metanólicos mediante cromatografía (HPLC) de las plantas resultantes.Resultados clave: La combinación para la producción de cápsulas con microestacas es alginato de sodio al 3% y cloruro de calcio dihidratado al 50 mM, con 25 minutos de polimerización. Las plántulas derivadas de semillas sintéticas, almacenadas por 100 días presentaron los mismos compuestos que las plantas donantes.Conclusiones: La encapsulación de microestacas de C. tenuiflora con alginato de sodio y cloruro de calcio dihidratado permite el almacenamiento del material vegetal y se mantienen sus compuestos principales, por lo que es una alternativa para mantener el genotipo de plantas elite y contrarrestar el saqueo de plantas silvestres.

show abstract

Untitled

Cited by 25 publications

References 5 publications

In vitro regeneration protocol for artificial seed production in an important medicinal plant Mentha arvensis L

In vitro regeneration protocol for artificial seed production in an important medicinal plant Mentha arvensis L

Sugarcane Bud Chip Encapsulation for ex vitro Synthetic Seed Formation

Optimización de las condiciones de producción de cápsulas con microestacas para aumentar el tiempo de almacenamiento de la planta medicinal mexicana Castilleja tenuiflora (Orobanchaceae)

Contact Info

Product

Resources

About