We present spectroscopic and photometric analysis of the quintuple star HD 86222 with an eclipsing component. Until now three visual components A, B, and C of this multiple star were known. Four components in the A/B pair were detected during the examination of the cross correlation functions obtained from the spectra. We noticed that the visual components A and B, separated by 0. 5, are in fact two binary stars -one eclipsing pair and one spectroscopic system. The pair with higher radial velocity amplitude corresponds to the eclipsing period. Photometric and spectroscopic observations of the eclipsing binary enable us to obtain the Wilson-Devinney model. The masses of this pair are M 1 = 1.29 ± 0.09 M and M 2 = 1.33 ± 0.09 M , respectively. The radii, slightly higher than for the main sequence stars, are R 1 = 1.35 ± 0.01 R and R 2 = 1.36 ± 0.01 R , respectively. The main parameters of the spectroscopic binary are also estimated, but they must be confirmed by future observations because of the uncertainty of the period. The farthest star named C, is 15 arc seconds from the main A and B components. Assuming that this object is gravitationally connected with the A and B binary pairs, HD 86222 has at least five components.