Uvaria rufa Blume attenuates benign prostatic hyperplasia via inhibiting 5α-reductase and enhancing antioxidant status

Buncharoen, Wararut; Saenphet, Kanokporn; Saenphet, Supap; Thitaram, Chatchote

doi:10.1016/j.jep.2016.10.036

Cited by 18 publications

(11 citation statements)

References 71 publications

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“…Phytochemical investigation found the presence of sterols in all four extracts. An important phytosterol, β-sitosterol, has previously been detected in ethyl acetate extracts from U. rufa stems [28]. The heartwood of C. sappan possessed high amounts of three phytosterols, campesterol, stigmasterol and beta-sitosterol [25].…”

Section: Discussionmentioning

confidence: 97%

“…Various parts of U. rufa also contained flavonoids, flavonols, alkaloids, and flavonolrutin, isoquercitrin, kaempferol, quercitrin and lignan glycoside [10][11][26][27]. β-sitosterol has been detected in ethyl acetate extracts from U. rufa stems [28]. Different types of flavonoids, alkaloids and sterols derived from various plant materials possessed α1-adrenergic receptor antagonists and exhibited relaxation effects on the dynamic component in the prostate gland of experimental animals [29][30][31][32].…”

Section: Introductionmentioning

confidence: 99%

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Relaxant Activities of Extracts from Uvaria rufa Blume and Caesalpinia sappan L. on Excised Rat’s Prostate Strips

Buncharoen

Saenphet

2019

JPRI

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Aims: To determine the relaxant activity of various extracts from the stems of Uraria rufa Blume and Caesalpinia sappan L. on rats’ prostate strips in vitro. Study Design: The relaxant efficacies of ethyl acetate and ethanolic extracts from the stems of U. rufa (UEA and UEOH) and C. sappan (CEA and CEOH) were tested on isolated rats’ prostate tissue pre-contracted by adrenaline. Place and Duration of Study: Department of Biology, Faculty of Science, Chiang Mai University, between February and September 2018. Methodology: A prostate strip was isolated, mounted in an organ bath filled with Krebs-Henseleit solution and induced to contract by adrenaline. The contracted strip was then exposed to each extract at 250 µg/mL for 30 minutes. The tension was recorded. Relaxant efficacies of various extracts were determined in prostate strips pre-contracted by adrenaline at 10 µM. All extracts were also determined for their bioactive components and the contents of total phenolics and total flavonoids. Results: The results showed that all of the extracts, as well as tamsulosin, a synthetic drug, exhibited relaxant effects (P < 0.001) on prostate smooth muscles. The UEA exhibited the most potency in relaxing the prostate smooth muscle with a maximal effect of 72.09 ± 2.15 %. The half-maximal effective concentration (EC50) values of the UEA, CEOH, UEOH and CEA were 140.23 ± 9.74, 226.35 ± 7.16, 235.35 ± 24.96 and 236.24 ± 5.05 µg/ml respectively, while tamsulosin was 86.83 ± 8.96 µg/ml. All extracts contained flavonoids, phenolics, sterols, tannins, phlobatannins, terpenoids, cardiac glycosides, alkaloids and reducing sugars. The highest contents of phenolics and flavonoids were found in CEOH and CEA respectively. Conclusion: We concluded that the ethyl acetate from the stems of U. rufa was the most potent in relaxing the prostate smooth muscles, and it may be useful to relieve the urological symptoms caused by benign prostatic hyperplasia (BPH).

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Relaxant Activities of Extracts from Uvaria rufa Blume and Caesalpinia sappan L. on Excised Rat’s Prostate Strips

Buncharoen

Saenphet

2019

JPRI

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“…While, the extract is poured into the eye in order to reduce the ophthalmic inflammation (Scartezzini and Speroni, 2000 ; Rahman et al, 2005 ). Likewise, in Sabah and Sarawak, western part of the Malaysia several Annonacaeae species including Enicosanthellum pulchrum, Friesodielsia latifolia, Uvaria grandi, Uvaria rufa are in used a remedy for pedal edema (Nordin et al, 2014 ), generalized body pain (Araujo et al, 2017 ), antipyretic (Parmar et al, 1994 ), and anti-inflammatory (Buncharoen et al, 2016 ). Moreover, in Africa 80% of the population is still dependent on the traditional remedies due to easy accessibility and low cost.…”

Section: Traditional Usesmentioning

confidence: 99%

Annonaceae: Breaking the Wall of Inflammation

2017

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Inventories of tropical forests have listed Annonaceae as one of the most diverse plant families. For centuries, it is employed in traditional medicines to cure various pathological conditions including snakebite, analgesic, astringent, diarrhea, dysentery, arthritis pain, rheumatism, neuralgia, and weight loss etc. Phytochemical analysis of Annonaceae family have reported the occurrence of alkaloids, flavonoids, triterpenes, diterpenes and diterpene flavone glycosides, sterols, lignans, and annonaceous acetogenin characteristically affiliated with Annonaceae sp. Numerous past studies have underlined the pleotropic pharmacological activities of the crude extracts and isolated compounds from Annonaceae species. This review is an effort to abridge the ethnobotany, morphology, phytochemistry, toxicity, and particularly focusing on the anti-inflammatory activity of the Annonaceae species.

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“…Although the molecular mechanism of BPH development has not been clearly elucidated, the general consensus is that increased steroid 5α-reductase activity is a risk factor for abnormal prostate growth and BPH development ( Sun et al, 2011 ). 5α-Reductase is membrane-bound enzyme in the oxidoreductase family that regulates steroid metabolism (particularly type 1 and type 2 5α-reductase) and irreversibly convert testosterone to dihydrotestosterone (DHT), which has a higher affinity for androgen receptors ( Buncharoen et al, 2016 ; Karnsomwan et al, 2017 ). When 5α-reductase is overexpressed, excessive DHT will dysregulate the hormone levels in the body, resulting in BPH, male pattern baldness, and acne ( Metcalf et al, 1989 ).…”

Section: Introductionmentioning

confidence: 99%

Characterization of carotenoids in Lycium barbarum fruit by using UPC2-PDA-Q-TOF-MSE couple with deep eutectic solvents extraction and evaluation of their 5α-reductase inhibitory activity

Xia²,

Li³

et al. 2022

Front. Chem.

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Carotenoids from Lycium barbarum fruits have possessed pharmacological efficacy against eye diseases, cardiovascular disorders, cancer, and benign prostatic hyperplasia. However, the efficient extraction, rapid characterization and activities evaluation of Lycium carotenoids remains a challenge. To concentrate and characterize Lycium carotenoids, we have developed ultrasound-assisted extraction methods with different deep eutectic solvents (DESs) and analyzed carotenoids by ultra-performance convergence chromatography coupled with photo diode array detector and quadrupole time-of-flight mass spectrometry (UPC2-PDA-Q-TOF-MSE). DESs containing choline chloride and malonic acid presented better extraction efficiency and were more environmentally friendly than other extraction methods. Carotenoids were more quickly profiled (in 11 min) by UPC2 compared to by UPLC (in 35 min), with seventeen main peaks were characterized in the MS fragmentation patterns. The in vitro 5α-reductase inhibitory activity of DESs extracts, fractions and components were subsequently assessed, and the predominant component zeaxanthin dipalmitate (ZD) exhibited potent inhibitory activity. Our study provides a chemical and pharmacological basis for the further development of potential new drugs based on Lycium carotenoids.

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Uvaria rufa Blume attenuates benign prostatic hyperplasia via inhibiting 5α-reductase and enhancing antioxidant status

Cited by 18 publications

References 71 publications

Relaxant Activities of Extracts from Uvaria rufa Blume and Caesalpinia sappan L. on Excised Rat’s Prostate Strips

Relaxant Activities of Extracts from Uvaria rufa Blume and Caesalpinia sappan L. on Excised Rat’s Prostate Strips

Annonaceae: Breaking the Wall of Inflammation

Characterization of carotenoids in Lycium barbarum fruit by using UPC2-PDA-Q-TOF-MSE couple with deep eutectic solvents extraction and evaluation of their 5α-reductase inhibitory activity

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