UV–Vis Spectrophotometric Analysis and Quantification of Glyphosate for an Interdisciplinary Undergraduate Laboratory

Felton, Daniel; Ederer, Martina M.; Steffens, Timothy; Hartzell, Patricia L.; Waynant, Kristopher V.

doi:10.1021/acs.jchemed.7b00440

Cited by 30 publications

(20 citation statements)

References 27 publications

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“…Capillary electrophoresis methods have been reported in recent years using detection systems such as contactless conductivity, electrochemiluminescence [100], and laser-induced fluorescence [101,102], as reviewed by Gauglitz et al [103]. Ion chromatography [104], electrochemical method, surface resonance-enhanced spectrometry, enzyme-linked immunosorbent assay also called ELISA methods [87], spectrophotometry [73,85], and fluorescent spectrometry [50,55,57,75,78,80,90,96] were also reported to detect GLY in current literatures. However, the selectivity of ion chromatography was limited.…”

Section: Other Methodsmentioning

confidence: 99%

Glyphosate Residues in Soil and Air: An Integrated Review

Tzanetou¹,

Karasali²

2020

Pests, Weeds and Diseases in Agricultural Crop and Animal Husbandry Production

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Glyphosate [N-(phosphonomethyl) glycine] (GPS) is currently the most commonly applied herbicide worldwide. Given the widespread use of glyphosate, the investigation of the relationship between glyphosate and soil ecosystem is critical and has great significance for its valid application and environmental safety evaluation. However, although the occurrence of glyphosate residues in surface and groundwater is rather well documented, only few information are available for soils and even fewer for air. Due to this, the importance of developing methods that are effective and fast to determine and quantify glyphosate and its major degradation product, aminomethylphosphonic acid (AMPA), is emphasized. Based on its structure, the determination of this pesticide using a simple analytical method remains a challenge, a fact known as the "glyphosate paradox." In this chapter a critical review of the existing literature and data comparison studies regarding the occurrence and the development of analytical methods for the determination of pesticide glyphosate in soil and air is performed.

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Section: Other Methodsmentioning

confidence: 99%

Glyphosate Residues in Soil and Air: An Integrated Review

Tzanetou¹,

Karasali²

2020

Pests, Weeds and Diseases in Agricultural Crop and Animal Husbandry Production

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“…Derivatization of Gly: Because Gly did not show clear absorbance in the UV range, in this study, FMOC-Cl was incorporated with Gly as per Felton's protocol. [19]. However, the solution of FMOC-Cl has a short half-life [20]; therefore, the stock solution of FMOC-CL was freshly storaged before measurement.…”

Section: Methods For Determining Gly Residues In Sugarcanementioning

confidence: 99%

“…Therefore, the detection of trace amounts of Gly in cane juice after application is very important in order to determine the future of the use of this herbicide in agricultural fields. Gly has high polarity, with the added feature of forming ionic species and no chromophores groups [19]. Thus, the determination of this compound often requires additional processes that may allow quantification through chromatographic methods.…”

Section: Evaluation Of Ga3 and Gly Residues In Sugarcanementioning

confidence: 99%

“…Thus, the determination of this compound often requires additional processes that may allow quantification through chromatographic methods. It is known that there are several studies on the determination of Gly in various types of samples, such as urine and breast milk [19,39]. In this study, the development of analytical methods for Gly was based on UV-Vis measurement.…”

Section: Evaluation Of Ga3 and Gly Residues In Sugarcanementioning

confidence: 99%

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Effect of GA3 and Gly Plant Growth Regulators on Productivity and Sugar Content of Sugarcane

Nguyen¹,

Dang²,

Nguyen³

et al. 2019

Agriculture

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The use of plant growth regulators is one effective solution to improve sugarcane yields and sugar content in several countries. In this study, we examined the role of gibberellin acid (GA3) and glyphosate (Gly) plant growth regulators to determine the appropriate concentration of GA3 and Gly to increase the yield of sugarcane and sugar accumulation, respectively. The statistical results showed that GA3 was sprayed at 150 ppm to increment the actual yield by 19.94%; sucrose accumulation increased by 2.21%. With Gly treatment, although the yield decreased by 3.17%, sucrose accumulation increased by 11.27% compared to control trials. In this study, the combined concentration of 150 ppm of both GA3 and Gly gave the best results, for which sucrose accumulation increased from 2.21% to 10.74% and from 19.94% to 20.97% for actual yield. The results led to increased net income compared to the control. To address concerns about residues of plant growth regulators, residues of GA3 and Gly were evaluated after the sugarcane harvest using the HPLC and UV-vis methods, respectively. The analyzed results showed that their residues were lower than what is permitted in several countries. This showed the applicability of the study, on a large scale, to increase sucrose accumulation, productivity of sugarcane, and profit for farmers.

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“…[5][6][7][8][9][10][11][12][13][14] Accordingly, the number of STEM courses and laboratory experiments designed to integrate multiple disciplines is increasing. [15][16][17][18][19][20][21] For example, the fields of biochemistry and molecular biophysics are inherently interdisciplinary in nature, and their principles encompass many concepts from biology, chemistry, and physics. 22 In this paper, we present an upper level interdisciplinary undergraduate biochemistry and molecular biophysics experiment.…”

Section: Introductionmentioning

confidence: 99%

Structural determination of model phospholipid membranes by Raman spectroscopy: Laboratory experiment

Giancaspro

Scollan

Rosario

et al. 2022

Biochem Molecular Bio Educ

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In an upper-division interdisciplinary laboratory experiment, students use Raman spectroscopy to highlight how the overall structure and conformational order of lipid bilayers can be influenced by their individual phospholipid composition. Students prepare a supported lipid bilayer, as a model cell membrane, by spreading liposomes made of various phospholipids on a solid support. The characterization of phospholipid bilayers, a major component of cellular membranes, can advance our fundamental understanding of important biological phenomena, with significant implications in various fields including drug delivery and development. We use Raman spectroscopy as an analytical tool to investigate the structural and packing properties of model cell membranes.The spectral frequency, intensity, and line-width of lipid Raman bands are extremely sensitive to structural alterations. This experimental module effectively exposes students to the fundamentals of Raman spectroscopy and teaches students the importance of interdisciplinary education as they integrate concepts from chemical structure, molecular interactions, and analytical spectroscopic techniques to gain a more holistic understanding of biological membrane properties.

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UV–Vis Spectrophotometric Analysis and Quantification of Glyphosate for an Interdisciplinary Undergraduate Laboratory

Cited by 30 publications

References 27 publications

Glyphosate Residues in Soil and Air: An Integrated Review

Glyphosate Residues in Soil and Air: An Integrated Review

Effect of GA3 and Gly Plant Growth Regulators on Productivity and Sugar Content of Sugarcane

Structural determination of model phospholipid membranes by Raman spectroscopy: Laboratory experiment

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