UV Resonance Raman Studies of Molecular Structure and Dynamics: Applications in Physical and Biophysical Chemistry

Asher, Sanford A.

doi:10.1146/annurev.pc.39.100188.002541

Cited by 207 publications

(154 citation statements)

References 64 publications

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“…This advance allows one to study a range from colored samples (generally showing fluorescence in the visible region) to colorless biomolecules (having absorption in the UV region, which allows the use of the resonance Raman effect) in solutions. 25,26 In the early 1990s, Stair and Li pioneeringly applied UV-resonance Raman spectroscopy (UV-RRS) to the study of catalysts (mainly metal oxides) having absorption in the UV region. 27,28 This extension of UV-Raman spectroscopy to a broader surface chemistry has far-reaching consequences.…”

Section: Introductionmentioning

confidence: 99%

Surface‐enhanced Raman spectroscopy with ultraviolet excitation

Lin

Ren

Yang

et al. 2005

J Raman Spectroscopy

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Surface-enhanced Raman scattering (SERS) spectroscopy excited with a UV laser was successfully developed and the UV-SER spectra of various adsorbates, including pyridine and SCN-, on different transition metal electrodes were obtained. The experimental requirements for obtaining UV-SERS in an electrochemical system are given. The surface enhancement factor of a roughened Rh electrode covered with thiocyanate as a model molecule was estimated to be about two orders of magnitude in the UV region, consistent with our preliminary theoretical calculation based on the electromagnetic model. The investigation of SERS in the UV region will improve the understanding of the SERS enhancement mechanism and broaden the research field of SERS in areas such as surface science and the life sciences. Copyright (c) 2005 John Wiley & Sons, Ltd

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Section: Introductionmentioning

confidence: 99%

Surface‐enhanced Raman spectroscopy with ultraviolet excitation

Lin

Ren

Yang

et al. 2005

J Raman Spectroscopy

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“…1a). Electronic resonance is known to significantly enhance Raman signals 16 , and moving from non-resonance (ω 0 >> ω pump ) to rigorous resonance (ω 0 = ω pump ) indeed enhances the detected stimulated Raman loss signal (apparent pump intensity decrease) of IR895 by a factor of about 10 8 compared to the C-O vibrational signal from methanol (cf. purple and yellow spectra in Fig.…”

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confidence: 99%

“…1) (Fig. 2a, red line):

σ = K ω_{0} {false(ω_{0} - ω_{vib} false)}^{3} {(\frac{false(ω_{pump}^{2} + ω_{0}^{2} false)}{{(ω_{0}^{2} - ω_{italicpump}^{2})}^{2}})}^{2}

where ω vib is the vibrational transition energy and K is a collection of frequency-independent factors of the dyes 16, 19 . We thus defined an optimal epr-SRS excitation region as 2Γ< ω 0 – ω pump <6Γ (Fig.…”

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confidence: 99%

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Super-multiplex vibrational imaging

Chen

Shi

et al. 2017

Nature

412

452

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The ability to directly visualize a large number of distinct molecular species inside cells is increasingly essential for understanding complex systems and processes. Even though existing methods have been used successfully to explore structural-functional relationships in nervous systems, profile RNA in situ, reveal tumor microenvironment heterogeneity or study dynamic macromolecular assembly1–4, it remains challenging to image many species with high selectivity and sensitivity under biological conditions. For instance, fluorescence microscopy faces a “color barrier” due to the intrinsically broad (~1500 cm−1) and featureless nature of fluorescence spectra5 that limits the number of resolvable colors to 2 to 5 (or 7-9 if using complicated instrumentation and analysis)6–8. Spontaneous Raman microscopy probes vibrational transitions with much narrower resonances (peak width ~10 cm−1) and thus doesn’t suffer this problem, but its feeble signals make many demanding bio-imaging applications impossible. And while surface-enhanced Raman scattering offers remarkable sensitivity and multiplicity, it cannot be readily used to quantitatively image specific molecular targets inside live cells9. Here we show that carrying out stimulated Raman scattering under electronic pre-resonance conditions (epr-SRS) enables imaging with exquisite vibrational selectivity and sensitivity (down to 250 nM with 1-ms) in living cells. We also create a palette of triple-bond-conjugated near-infrared dyes that each display a single epr-SRS peak in the cell-silent spectral window, and that with available fluorescent probes give 24 resolvable colors with potential for further expansion. Proof-of-principle experiments on neuronal co-cultures and brain tissues reveal cell-type dependent heterogeneities in DNA and protein metabolism under physiological and pathological conditions, underscoring the potential of this super-multiplex optical imaging approach for untangling intricate interactions in complex biological systems.

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“…[1][2][3][4][5][6] The main drawback associated with UV-resonance Raman spectroscopy is photodegradation of the samples during laser exposure, which can pose a serious problem for the observation of actual biological processes, especially when the observation volume cannot be refreshed, as is the case with microscopic imaging. 7,8 In order to develop non-destructive UV-resonance Raman microscopic measurement methods, the origin of UV photodegradation during spectroscopic measurements must be more clearly defined.…”

Section: Introductionmentioning

confidence: 99%