Temperature-dependent Photodegradation in UV-resonance Raman Spectroscopy

Yoshino, Hikaru; Saito, Yuika; Kumamoto, Yoshiaki; Taguchi, Akihito; Verma, Prabhat; Kawata, Satoshi

doi:10.2116/analsci.31.451

Cited by 3 publications

(3 citation statements)

References 22 publications

(18 reference statements)

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“…176−180 The energy relaxation also helps to avoid generation of reactive oxygen species from surrounding medium, which otherwise degrades nearby molecules. 176,177 Oxygen purging 176,177 and sample cooling 184,185 have been also used to suppress the deep-UV photodegradation.…”

Section: Deep-uv Resonant Raman Scatteringmentioning

confidence: 99%

“…This protection effect is explained by Förster resonance energy transfer (FRET) between the excited molecules and the lanthanide ions. The nucleotide bases and aromatic amino acids are excited by the absorption of deep-UV light, and the energy is relaxed via FRET, before the molecule is irreversibly broken via ionization, radicalization, oxidation, cleavage, and/or polymerization. − The energy relaxation also helps to avoid generation of reactive oxygen species from surrounding medium, which otherwise degrades nearby molecules. , Oxygen purging , and sample cooling , have been also used to suppress the deep-UV photodegradation.…”

Section: Enhancement By Electronic Resonancementioning

confidence: 99%

See 1 more Smart Citation

Nano-Raman Scattering Microscopy: Resolution and Enhancement

Kawata

Ichimura²,

Taguchi

et al. 2017

Chem. Rev.

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Raman scattering microscopy is becoming one of the hot topics in analytical microscopy as a tool for analyzing advanced nanomaterials, such as biomolecules in a live cell for the study of cellular dynamics, semiconductor devices for characterizing strain distribution and contamination, and nanocarbons and nano-2D materials. In this paper, we review the recent progress in the development of Raman scattering microscopy from the viewpoint of spatial resolution and scattering efficiency. To overcome the extremely small cross section of Raman scattering, we discuss three approaches for the enhancement of scattering efficiency and show that the scattering enhancement synergistically increases the spatial resolution. We discuss the mechanisms of tip-enhanced Raman scattering, deep-UV resonant Raman scattering, and coherent nonlinear Raman scattering for micro- and nanoscope applications. The combinations of these three approaches are also shown as nanometer-resolution Raman scattering microscopy. The critical issues of the structures, materials, and reproducibility of tips and three-dimensionality for TERS; photodegradation for resonant Raman scattering; and laser availability for coherent nonlinear Raman scattering are also discussed.

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Section: Deep-uv Resonant Raman Scatteringmentioning

confidence: 99%

Section: Enhancement By Electronic Resonancementioning

confidence: 99%

Nano-Raman Scattering Microscopy: Resolution and Enhancement

Kawata

Ichimura²,

Taguchi

et al. 2017

Chem. Rev.

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“…Recently, Yoshino et al suppressed molecular degradation occurring under UV (λ = 355 nm) light exposure. They showed that deep cooling down to 80 K decreased chemical reaction rates of electronically excited biomolecules . Their study is not in the DUV, but implies that deep cooling can suppress molecular degradation under DUV exposure by decreasing reaction rates of electronically excited biomolecules.…”

Section: Photodamagementioning

confidence: 95%

Deep‐Ultraviolet Biomolecular Imaging and Analysis

Kumamoto

Taguchi

Kawata

2018

Advanced Optical Materials

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Deep‐ultraviolet light, 200–300 nm in wavelength, interacts with nucleic acids and proteins strongly compared to visible and infrared light. In this article, the interaction between deep‐ultraviolet photons and biomolecules is discussed. Especially, the absorption and autofluorescence of biomolecules by the deep‐ultraviolet excitation are examined. Applications of deep‐ultraviolet absorption and autofluorescence to label‐free biomolecular imaging and analysis of cells and tissues are shown. Resonant Raman scattering at nucleotide bases and aromatic amino acids as another important topic of deep‐ultraviolet photonics is reviewed in biomolecular imaging and analysis. The discussion extends to the recent progress in the development of deep‐ultraviolet microscopes as well as a variety of deep‐ultraviolet optical devices such as light sources, detectors, and objectives. The issue of photodamage due to deep‐ultraviolet irradiation on cells and tissues is also discussed. It has been recently suppressed with use of lanthanide ions. The experimental results of the photodamage suppression are shown. For surface‐enhanced resonant Raman scattering, autofluorescence enhancement, and tip‐enhanced resonant Raman scattering microscopy, plasmonic materials applicable in the deep‐ultraviolet range are discussed.

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Deep-ultraviolet microscopy for tryptophan label-free imaging in cells and tissue

Kumamoto¹

2022

Biophotonics, Tryptophan and Disease

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Temperature-dependent Photodegradation in UV-resonance Raman Spectroscopy

Cited by 3 publications

References 22 publications

Nano-Raman Scattering Microscopy: Resolution and Enhancement

Nano-Raman Scattering Microscopy: Resolution and Enhancement

Deep‐Ultraviolet Biomolecular Imaging and Analysis

Deep-ultraviolet microscopy for tryptophan label-free imaging in cells and tissue

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