UV photoprotection by combination topical antioxidants vitamin C and vitamin E

Lin, Jheng-Wei; Selim, M. Angélica; Shea, Christopher R.; Grichnik, James M.; Omar, Mostafa M.; Monteiro‐Riviere, Nancy A.; Pinnell, Sheldon R.

doi:10.1067/mjd.2003.425

Cited by 294 publications

(220 citation statements)

References 57 publications

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“…This methodology was previously validated as reported in the literature. [24][25][26] SPC quantification SPC concentration was determined by an enzymatic and colorimetric method (Phospholipids kit; Spinreact, Sant Esteve de Bas, Spain) at 505 nm and 37°C.…”

Section: Characterization Of Udv Formulationsmentioning

confidence: 99%

Development, characterization, and skin delivery studies of related ultradeformable vesicles: transfersomes, ethosomes, and transethosomes

Ascenso¹,

Raposo²,

Batista³

et al. 2015

IJN

242

150

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Ultradeformable vesicles (UDV) have recently become a promising tool for the development of improved and innovative dermal and transdermal therapies. The aim of this work was to study three related UDV: transfersomes, ethosomes, and transethosomes for the incorporation of actives of distinct polarities, namely, vitamin E and caffeine, and to evaluate the effect of the carrier on skin permeation and penetration. These actives were incorporated in UDV formulations further characterized for vesicles imaging by transmission electron microscopy; mean vesicle size and polydispersity index by photon correlation spectroscopy; zeta potential by laser-Doppler anemometry; deformability by pressure-driven transport; and incorporation efficiency (IE) after actives quantification by high-performance liquid chromatography. Topical delivery studies were performed in order to compare UDV formulations regarding the release, skin permeation, and penetration profiles. All UDV formulations showed size values within the expected range, except transethosomes prepared by “transfersomal method”, for which size was smaller than 100 nm in contrast to that obtained for vesicles prepared by “ethosomal method”. Zeta potential was negative and higher for formulations containing sodium cholate. The IE was much higher for vitamin E- than caffeine-loaded UDV as expected. For flux measurements, the following order was obtained: transethosomes (TE) > ethosomes (E) ≥ transfersomes (T). This result was consistent with the release and skin penetration profiles for Vitamin E-loaded UDV. However, the releasing results were totally the opposite for caffeine-loaded UDV, which might be explained by the solubility and thermodynamic activity of this active in each formulation instead of the UDV deformability attending to the higher non-incorporated fraction of caffeine. Anyway, a high skin penetration and permeation for all caffeine-loaded UDV were obtained. Transethosomes were more deformable than ethosomes and transfersomes due to the presence of both ethanol and surfactant in their composition. All these UDV were suitable for a deeper skin penetration, especially transethosomes.

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Section: Characterization Of Udv Formulationsmentioning

confidence: 99%

Development, characterization, and skin delivery studies of related ultradeformable vesicles: transfersomes, ethosomes, and transethosomes

Ascenso¹,

Raposo²,

Batista³

et al. 2015

IJN

242

150

View full text Add to dashboard Cite

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“…Specifically, L-ascorbic acid has been shown to reduce UV-induced photodamage in a porcine skin model (27) and human cells (28). Notably, Lin et al have developed a topical formulation consisting of 15% L-ascorbic acid combined with 1% DL-α-tocopherol (17). When DL-α-tocopherol neutralizes oxidative stress in lipids, its oxidation product can be regenerated by L-ascorbic acid (28)(29)(30)(31)(32)(33)(34)(35)(36).…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, the identification of novel strategies aimed at reducing UVB-induced damage in human keratinocytes has been previously studied (12)(13)(14). Several antioxidants and cytoprotective compounds, including L-carnosine, L-(+)-ergothioneine, L-ascorbic acid and DL-α-tocopherol, have been shown to reduce UV-induced injury in experimental and animal models (15)(16)(17)(18). In contrast to the DNA alterations that may lead to NMSC-related mutations, growing evidence indicates that UVB radiation can induce protein carbonylation (PC), a major form of oxidative protein damage that has been recently proposed as a critical molecular signature of skin carcinogenesis (19).…”

Section: Introductionmentioning

confidence: 99%

Protective effect of trehalose-loaded liposomes against UVB-induced photodamage in human keratinocytes

Emanuele¹,

Bertona²,

Sanchís-Gomar

et al. 2014

Biomedical Reports

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Abstract. Trehalose, a naturally occurring non-reducing disaccharide, is known to act as a major protein stabilizer that can reduce ultraviolet B (UVB)-induced corneal damage when topically applied to the eye. However, due to the low skin permeability of trehalose, which makes the development of topical formulations difficult, its use as a skin photoprotective agent has been limited. Previous findings demonstrated that liposomes may significantly improve the intracellular delivery of trehalose. Therefore, the present study aimed to assess the protective effects of trehalose-loaded liposomes against UVB-induced photodamage using the immortalized human keratinocyte cell line, HaCaT. The effects were also compared to those of the common skin photoprotective compounds, including L-carnosine, L-(+)-ergothioneine, L-ascorbic acid and DL-α-tocopherol. The levels of cyclobutane pyrimidine dimers, 8-hydroxy-2'-deoxyguanosine and protein carbonylation in HaCaT cells were used as biological markers of UVB-induced damage. Compared to other compounds, trehalose-loaded liposomes showed the highest efficacy in reducing the levels of the three markers following UVB irradiation of HaCaT cells (all P<0.001 when compared to each of the four other photoprotective compounds). Therefore, these findings indicate that there may be a clinical application for trehalose-loaded liposomes, and further studies should be performed to assess the potential usefulness in skin photoprotection and the prevention of non-melanoma skin cancer.

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“…Vitamin B complex is involved in cell metabolism and allows scavenge ROS; Vitamin C acts as a very important antioxidant and induces collagen synthesis [12]. Vitamin E is an antioxidant and moisturizer compound [13]. Topical antioxidants can down-regulate free radical-mediated pathways that damage skin [14].…”

Section: Discussionmentioning

confidence: 99%

Clinical Assessment of a Mesotherapy Formulation for Skin Rejuvenation in Healthy Volunteers

Grand-Vincent¹,

Boisnic²,

Salomon³

et al. 2017

JCDSA

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Mesotherapy is a mildly invasive procedure involving several subcutaneous drug injections, which are performed simultaneously. The concept is to inject a very small amount of active compounds (hyaluronic acid, vitamins, antioxidants, enzymes, humectants, …) and the aim is to stimulate the biosynthetic capacity of fibroblasts, increase collagen and elastin production and to give active essential molecules for a fibroblasts environment. Numerous studies suggest that the mesotherapy procedure improves skin hydration and mechanical properties; however, some results have not been statistically significant. In this study, we investigated an antiaging treatment as a complementary approach to a non-surgical rejuvenating procedure after 5 sessions of mesotherapy. Our main objective was to assess the improvement of face, neck and neckline skin quality, after intradermal microinjections of NCTF 135HA®. This mixture, manufactured by FILORGA Laboratories, includes a non-cross-linked hyaluronic acid, vitamins, antioxidants, mineral salts and enzymes. A statistically significant decrease in the depth of crow's-feet wrinkles (43.3%) and in pore size (58.5%) was observed. Also, dermatological scores showed a significant decrease, and data suggest that skin tone was improved with a brighter tone. The injected mixture could then have an action on the skin pigmentation process with a photoprotective effect. Evolution of Dermis density was also investigated and an increase of dermal density and thickness were highlighted, showing an improvement of skin firmness with significant increases of dermis thickness (20%) and dermis density (24%), indicating a neocollagen activity.

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UV photoprotection by combination topical antioxidants vitamin C and vitamin E

Cited by 294 publications

References 57 publications

Development, characterization, and skin delivery studies of related ultradeformable vesicles: transfersomes, ethosomes, and transethosomes

Development, characterization, and skin delivery studies of related ultradeformable vesicles: transfersomes, ethosomes, and transethosomes

Protective effect of trehalose-loaded liposomes against UVB-induced photodamage in human keratinocytes

Clinical Assessment of a Mesotherapy Formulation for Skin Rejuvenation in Healthy Volunteers

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