UV light-emitting diode (UV-LED) at 265 nm as a potential light source for disinfecting human platelet concentrates

Abstract

The risk of sepsis through bacterial transmission is one of the most serious problems in platelet transfusion. In processing platelet concentrates (PCs), several methods have been put into practice to minimize the risk of bacterial transmission, such as stringent monitoring by cultivation assays and inactivation treatment by photoirradiation with or without chemical agents. As another potential option, we applied a light-emitting diode (LED) with a peak emission wavelength of 265 nm, which has been shown to be effective for water, to disinfect PCs. In a bench-scale UV-LED exposure setup, a 10-min irradiation, corresponding to an average fluence of 9.2 mJ/cm2, resulted in >2.0 log, 1.0 log, and 0.6 log inactivation (mean, n = 6) of Escherichia coli, Staphylococcus aureus, and Bacillus cereus, respectively, in non-diluted plasma PCs. After a 30-min exposure, platelet counts decreased slightly (18 ± 7%: mean ± SD, n = 7); however, platelet surface expressions of CD42b, CD61, CD62P, and PAC-1 binding did not change significantly (P>0.005), and agonist-induced aggregation and adhesion/aggregation under flow conditions were well maintained. Our findings indicated that the 265 nm UV-LED has high potential as a novel disinfection method to ensure the microbial safety of platelet transfusion.

UV C Light from a Light-Emitting Diode at 275 Nanometers Shortens Wound Healing Time in Bacterium- and Fungus-Infected Skin in Mice

“…The emergence of the light-emitting diode (LED) light source provides a new option for UVC sterilization and realizes the continuously tunable wavelength ( 40 ). There has been limited research on the 265-nm wavelength ( 34 , 41 – 45 ) and 280-nm wavelength ( 46 – 49 ) for treatment recently. However, no literature reported the in vivo application and safety of 275-nm UVC light in antimicrobial therapy ( 32 ).…”

Towards Efficient Electrically-Driven Deep UVC Lasing: Challenges and Opportunities

“…For example, UVC light below 280 nm wavelength has a strong biocidal effect against protozoa, bacteria, viruses, and molds, destroying or inactivating microorganisms so that they can no longer multiply. Light at wavelengths near 265 nm is the most effective for producing this effect [ 1 , 2 ]. Light at 280 nm wavelength and below is also needed for non-line-of-sight urban communication applications [ 3 ] and can be potentially used in deep UV lithography.…”