Here we present the UV-induced disulfide formation (UV-DF) and disulfide reduction (UV-DRSpatially resolved functionalization of surfaces is important in a variety of research fields ranging from microfluidics [1] and electronics [2] to biotechnology. [3] In this context, photochemical reactions attracted attention, because they offer both spatial and temporal control of surface modification. [4] To date, a variety of light-triggered reactions, for example, thiol-ene [5] /yne, [6] photoinitiated azide-yne, [7] tetrazole-ene, [8] tetrazole-thiol, [9] and phototriggered Diels-Alder reactions, [10] have been exploited for surface functionalization. However, the majority of the existing methods are restricted to irreversible surface functionalization because of the formation of relatively non-reactive covalent bonds. In contrast, reversible photochemical modifications provide more diverse possibilities for surface manipulation. These can be applied for example, for dynamic tuning of interfacial properties, stimuli-responsive activation and deactivation of specific functions, or reversible attachment/detachment of functional moieties.To the best of our knowledge, only three examples of reversible phototriggered surface functionalization methods based on thiol−quinone methide reaction, [11] addition fragmentation chain transfer reaction of allyl sulfides with thiols, [12] and photodynamic disulfide exchange reaction (PDDE), [13] have been reported. Here we demonstrate two photo-induced reactions allowing for the spatially and temporally controlled thiol-disulfide interconversions, namely UVinduced disulfide formation (UV-DF) and disulfide reduction (UV-DR) reactions. These reactions are used to demonstrate reversible UV-induced surface modification, patterning, attachment as well as detachment of functional molecules (Scheme 1).Thiol-disulfide interconversion is well-documented to proceed via the thiolate-centered mechanism; [14] however, it lacks the spatial control required for surface patterning. Recently,