UV detection of derivatized carbonyl compounds in rain samples in capillary electrophoresis using sample stacking and a Z-shaped flow cell

Mainka, A.; Bächmann, K.

doi:10.1016/s0021-9673(97)00012-5

Cited by 42 publications

(35 citation statements)

References 20 publications

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“…For example, because of light attenuation, only a sixfold S/N ratio was observed despite a 60 times longer pathlength for the capillary modified Z-cell [7]. Yet, using a Z-cell, UV detection and chemical derivatization of the analytes, an improvement in the detection limit by a factor of 20 was achieved for the trace analysis of carbonyl compounds in rain water as reported by Bachmann and Mainka [8] (Fig. 1).…”

Section: Direct Measurementsmentioning

confidence: 62%

Detection in capillary electrophoresis

2000

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Section: Direct Measurementsmentioning

confidence: 62%

Detection in capillary electrophoresis

2000

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“…Most of the published protocols for DNSH derivatisation report 50 C as the reaction temperature. 23,25 Parallel to this, we tested other temperatures to investigate the possible gain in terms of the reaction time and the derivative yield. After a preliminary test with temperatures ranging from room temperature to 100 C, 80 C was chosen owing to its better reaction time/yield ratio (data not shown), and subsequently compared to 50 C. Interestingly, a reaction only between KG and DNSH at room temperature could be observed.…”

Section: Optimization Of the Derivatization Reactionmentioning

confidence: 99%

Simultaneous Quantitation of Alpha-ketoglutaric Acid and 5-Hydroxymethylfurfural in Plasma by HPLC with UV and Fluorescence Detection

et al. 2013

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Alpha-ketoglutaric acid (KG) and hydroxymethylfurfural (HMF) are currently being investigated in clinical trials as an approach in targeted cancer therapy. Hence, a method for the simultaneous determination of KG and HMF in plasma has been developed. Due to the strongly discriminative chemical properties of KG and HMF, SPE purification is performed using an ion-exchange cartridge to separate KG, and a hydrophobic polymeric cartridge to separate HMF. The cartridges are connected together for several steps, thus resulting in a quicker approach for the purification of plasma samples. The derivatization step is based on the reaction of the carbonyl groups of KG and HMF with dansylhydrazine (DNSH) catalyzed by trifluoroacetic acid. The formed derivatives could be separated by reversed-phase LC on a C8-column, and analyzed by UV and fluorescence detection in a single run using a gradient program. The obtained results show good reproducibility, specificity, and detection limits down to the low picomole range.

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“…Aldehydes are carbonyl compounds widely distributed in nature and their concentrations were determined in many matrices such as food and beverages [1,2], air [3,4] and water [5][6][7]. These ubiquitous compounds are known to be irritant, carcinogenic, capable of binding to protein and nucleic acids and impairing their functions [8].…”

Section: Introductionmentioning

confidence: 99%

“…(pentafluorobenzyl)hydroxylamine (PFBHA) [5,15], dansylhydrazine (DNSH) [7], 4-(N,N-dimethylaminosulphony)-7-hydrazino-2,1,3-benzoxadiazole (DBD-H) [16] and 1,3,5,7-tetramethyl-8-aminozidedifluoroboradiaza-s-indacene (BODIPY-aminozide) [17] have been developed. Although various reagents have been used so far but some of them lack selectivity and other carbonyl compounds may interfere during aldehyde determination especially in biological fluids.…”

Section: Introductionmentioning

confidence: 99%

Chromatographic determination of aliphatic aldehydes in human serum after pre-column derivatization using 2,2′-furil, a novel fluorogenic reagent

Ali

Kishikawa

Ohyama

et al. 2013

Journal of Chromatography A

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A novel, highly sensitive and selective fluorimetric liquid chromatographic method for simultaneous determination of medium chain aliphatic aldehydes was developed. The method was based on the derivatization of aliphatic aldehydes with 1,2-di(2-furyl)-1,2-ethanedione (2,2`-furil), a novel fluorogenic reagent, to form highly fluorescent difurylimidazole derivatives. The fluorescence derivatives were separated in less than 20 min on a reversed-phase ODS column using an isocratic elution with a mixture of methanol-water (80:20, v/v%). The detection limits were from 0.19 to 0.50 nM (1-10 fmol/injection) at a signal-tonoise ratio (S/N) of 3. This method was successfully applied for monitoring of aliphatic aldehydes in healthy human sera by a simple pretreatment procedure without interferences from serum constituents.

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UV detection of derivatized carbonyl compounds in rain samples in capillary electrophoresis using sample stacking and a Z-shaped flow cell

Cited by 42 publications

References 20 publications

Detection in capillary electrophoresis

Detection in capillary electrophoresis

Simultaneous Quantitation of Alpha-ketoglutaric Acid and 5-Hydroxymethylfurfural in Plasma by HPLC with UV and Fluorescence Detection

Chromatographic determination of aliphatic aldehydes in human serum after pre-column derivatization using 2,2′-furil, a novel fluorogenic reagent

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