Chromatographic determination of aliphatic aldehydes in human serum after pre-column derivatization using 2,2′-furil, a novel fluorogenic reagent

Abstract

A novel, highly sensitive and selective fluorimetric liquid chromatographic method for simultaneous determination of medium chain aliphatic aldehydes was developed. The method was based on the derivatization of aliphatic aldehydes with 1,2-di(2-furyl)-1,2-ethanedione (2,2`-furil), a novel fluorogenic reagent, to form highly fluorescent difurylimidazole derivatives. The fluorescence derivatives were separated in less than 20 min on a reversed-phase ODS column using an isocratic elution with a mixture of methanol-water (80:20, v/v%). The detection limits were from 0.19 to 0.50 nM (1-10 fmol/injection) at a signal-tonoise ratio (S/N) of 3. This method was successfully applied for monitoring of aliphatic aldehydes in healthy human sera by a simple pretreatment procedure without interferences from serum constituents.

Current trends in isotope‐coded derivatization liquid chromatographic‐mass spectrometric analyses with special emphasis on their biomedical application

“…Hydrazine reagents are unstable, flammable, irritant to skin, and toxic to nerves and respiratory tract (Fathy Bakr Ali et al, ). Thus, many reagents that do not belong to the hydrazine‐based group were developed and adopted for the ICD of carbonyl compounds.…”

“…Their derivatization reaction depends on the coupling of aldehydes with an alpha‐dicarbonyl compound and ammonium acetate to form a fluorescence and strongly ionizable imidazole derivative. This derivatization technique was applied for determination of various types of aldehydes and alpha carbonyl aldehydes in biological samples (El‐Maghrabey, Kishikawa, & Kuroda, ; El‐Maghrabey, Kishikawa, Ohyama, & Kuroda, ; El‐Maghrabey, Nakatani, Kishikawa, & Kuroda, ; Fathy Bakr Ali et al, ). Recently, a novel ICD method for aldehydes was developed by the same research group based on the same idea using commercially available 15 N 0 / 15 N 1 ‐ammonium acetate and 9,10‐phenanthrenequinone.…”

“…Hydrazine reagents are unstable, flammable, irritant to skin, and toxic to nerves and respiratory tract (Fathy Bakr Ali et al, 2013).…”

New Methyltrimethoxysilane-(3-Mercaptopropyl)-Trimethoxysilane Coated Hollow Fiber-Solid Phase Microextraction for Hexanal and Heptanal Analysis

“…Kata kunci: pengekstrakan mikro fasa pepejal-gentian berongga, hibrid sol-gel, metiltrimetoksisilana, (3-merkaptopropil)-trimetoksisilana, heksanal, heptanal Introduction Aldehydes are common carbonyl compounds that take part in the pathology of certain diseases and their presence have been suggested as degradation products of lipid peroxidation [1,2]. Besides, aldehydes were predominately VOCs biomarker found at elevated concentrations level in lung cancer subjects.…”

“…Kesesuaian HF-SPME MTMOS-MPTMOS untuk analisis heksanal dan heptanal dalam sampel air kencing manusia terbukti dengan perolehan kuantitatif yang dicapai (> 90%). HF-SPME MTMOS-MPTMOS menawarkan alternatif menarik sebagai alat pengekstrakan yang cepat dan mudah serta menunjukkan potensi yang baik untuk analisis heksanal dan heptanal daripada sampel akueus.Kata kunci: pengekstrakan mikro fasa pepejal-gentian berongga, hibrid sol-gel, metiltrimetoksisilana, (3-merkaptopropil)-trimetoksisilana, heksanal, heptanal Introduction Aldehydes are common carbonyl compounds that take part in the pathology of certain diseases and their presence have been suggested as degradation products of lipid peroxidation [1,2]. Besides, aldehydes were predominately VOCs biomarker found at elevated concentrations level in lung cancer subjects.…”