UV Absorbance of Aqueous DNA

Abstract

Abstract:The UV absorption spectra of water with different electric charge (potential) were obtained. It was shown that UV absorption spectra of water with negative electric charge (potential) have the sharp peaks with maximum in the range 190 -200 nm and UV absorption spectra of water with positive electric charge (potential) have the wide peaks with maximum in the range 200 -220 nm. It was asked to explain this absorption. It was also established that UV absorption spectra of water solutions of surface inactive substances have sharp peaks with a maximum in the range 190 -200 nm and UV absorption spectra of solutions of surface active substances have the wide peaks with a maximum in the range 200 -220 nm. The UV absorption spectra of DNA solutions, which were prepared on the water with different electric charge (potential), were obtained. It was shown that these spectra are dependent on the electric charge (potential) of water used. It was proposed that UV absorption spectra of aqueous DNA reflect mostly the spectral properties of charged water or charged cuvette.

The Significance of Positive Electrization on Lifting Force

“…On the contrary, the negative electrification of water leads to the appearance of a peak with a maximum at ~ 195 nm on the same spectra. It should also be taken into account that uncharged water does not have a significant absorption of UV radiation in the range from 180 to 240 nm [12]. As a result, using this relationship, it was found that the contact of water with these feathers is always accompanied by the appearance of significant absorption at 205 -210 nm on the UV spectra of water absorption.…”

Legends of DNA morphology.

“…Feather research perspectives Based on the results obtained, to create a DNA model that better reflects the morphological features of real DNA. So, it can be waiting that thanks to the most successfully DNA models can be explained of a number of properties of real DNA, including their melting, as well as the nature of hypo-and hyperchromic effects in the UV absorption spectra of aqueous DNA solutions [4,[28][29][30].…”

“…Precipitation was carried out in open vials stored in the dark. Under such conditions, the solution acquires a negative potential, which reduces the solubility of DNA and promotes its precipitation [4][5][6].…”

“…The needle-shaped crystals of NaCl[4]. Potassium chloride does not form such needle-shaped crystals under the same conditions.…”

“…The formation of salt crystals under various conditions has been studied in detail. In particular, it was shown that the shape of the crystals is very sensitive to the conditions in which they form [4][5][6][7][8]. It was also shown that the shapes of the crystals are highly dependent on the properties of the surfaces on which crystallization occurs.…”