Utilizing Dynamic Light Scattering as a Process Analytical Technology for Protein Folding and Aggregation Monitoring in Vaccine Manufacturing

Combustion synthesis and characterization of NiO nanoparticles

“…4(a) and (b). The vast difference in the values can be attributed to hydrodynamic diameter of NiO NPs which include solvation layers [50]. Since the scattering intensity is directly proportional to the sixth power of the particle radius, this technique is extremely sensitive towards the presence of small aggregates and dense materials like metal oxides resulting in overestimation of particle size and conflicting data [51,52].…”

Recent applications of light scattering measurement in the biological and biopharmaceutical sciences

“…However, unfolding does lead to changes in DLS, which is sensitive to changes in scatterer size. We are aware of only one publication reporting the use of DLS to monitor chemical denaturation, and the work presented is only preliminary [20]. The technique is promising and deserves further development.…”

Wanted: more monitoring and control during inclusion body processing

“…Yu et al (2013) used dynamic light scattering ( DLS ) to monitor the refolding process of a protein-based vaccine candidate. As this method has no need for an intrinsic or extrinsic fluorophore, it is not restricted to proteins with distinct properties.…”

“…As DLS measurements are non-intrusive, aggregation or dissociation caused by the assay is greatly decreased. Concerning the precision of the rH values (particle size information) Yu et al (2013) reported that a difference of 1.5 nm is reliable. Protein concentrations of 0.05–0.1 g/l were reported to give the best results (Bhattacharjee 2016).…”

“…A small drawback is that the samples need to be filtered because particles and lipopolysaccharide micelles have to be removed before the measurements. Yu et al (2013) found that DLS data can also be used for quantification of refolded protein because the obtained measurements can be correlated to SE-HPLC results. In summary, DLS has many characteristics making it an ideal PAT tool.…”