Utilization potential of agro-industrial by-products and waste sources: Laccase production in bioreactor with Pichia pastoris

Çaloğlu, Buse; Bi̇nay, Barış

doi:10.1016/j.bej.2023.108854

Cited by 7 publications

(3 citation statements)

References 45 publications

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“…Interestingly, a remarkable increase in protein content (46.36–59.75%) was observed upon the addition of α-amylase, which implies that it is effective in the recovery of fermentable sugars such as glucose [ 54 ]. These results correlate with other scholarly reports because edible fungus strains such as F. venenatum can produce up to 50% of the protein, and these biocatalysts are already used in large-scale SCP processes—an example includes the commercial Quorn™ brand, which is produced by Marlow Foods [ 55 ].…”

Section: Nutritional Composition Of Food Waste and Its Suitability Fo...supporting

confidence: 86%

Valorization of Food Waste into Single-Cell Protein: An Innovative Technological Strategy for Sustainable Protein Production

Sekoai,

Roets-Dlamini,

O’Brien

et al. 2024

Microorganisms

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The rapidly increasing population and climate change pose a great threat to our current food systems. Moreover, the high usage of animal-based and plant-based protein has its drawbacks, as these nutritional sources require many hectares of land and water, are affected by seasonal variations, are costly, and contribute to environmental pollution. Single-cell proteins (SCPs) are gaining a lot of research interest due to their remarkable properties, such as their high protein content that is comparable with other protein sources; low requirements for land and water; low carbon footprint; and short production period. This review explores the use of food waste as a sustainable feedstock for the advancement of SCP processes. It discusses SCP studies that exploit food waste as a substrate, alongside the biocatalysts (bacteria, fungi, yeast, and microalgae) that are used. The operational setpoint conditions governing SCP yields and SCP fermentation routes are elucidated as well. This review also demonstrates how the biorefinery concept is implemented in the literature to improve the economic potential of “waste-to-protein” innovations, as this leads to the establishment of multiproduct value chains. A short section that discusses the South African SCP scenario is also included. The technical and economic hurdles facing second-generation SCP processes are also discussed, together with future perspectives. Therefore, SCP technologies could play a crucial role in the acceleration of a “sustainable protein market”, and in tackling the global hunger crisis.

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Section: Nutritional Composition Of Food Waste and Its Suitability Fo...supporting

confidence: 86%

Valorization of Food Waste into Single-Cell Protein: An Innovative Technological Strategy for Sustainable Protein Production

Sekoai,

Roets-Dlamini,

O’Brien

et al. 2024

Microorganisms

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“…In addition to these approaches, optimization of culture medium, fermentation process and strain design including metabolic pathway engineering could be effective in refining the methylotrophic yeast heterologous protein production system. From a bioeconomy perspective, these yeast systems should also develop to produce the desired bioproduct with sustainable manufacturing process in an environmentally friendly manner (Tülek et al 2021 ; Ergün et al 2021 , 2022 ; Çaloğlu and Binay 2023 ). Regarding gene editing techniques, the recently established CRISPR/Cas9 system has been rapidly developed and widely adopted as a powerful and accurate tool in K. phaffii and O. polymorpha (Weninger et al 2016 ; Manfrão-Netto et al 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Recent progress on heterologous protein production in methylotrophic yeast systems

Tsuda,

Nonaka

2024

World J Microbiol Biotechnol

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Recombinant protein production technology is widely applied to the manufacture of biologics used as drug substances and industrial proteins such as recombinant enzymes and bioactive proteins. Various heterologous protein production systems have been developed using prokaryotic and eukaryotic hosts. Especially methylotrophic yeast in eukaryotic hosts is suggested to be particularly valuable because such systems have the following advantages: protein secretion into culture broth, eukaryotic quality control systems, a post-translational modification system, rapid growth, and established recombinant DNA tools and technologies such as strong promoters, effective selection markers, and gene knock-in and -out systems. Many methylotrophic yeasts such as the genera Candida, Ogataea, and Komagataella have been studied since methylotrophic yeast was first isolated in 1969. The methanol-consumption-related genes in methylotrophic yeast are strongly and strictly regulated under methanol-containing conditions. The well-regulated gene expression systems under the methanol-inducible gene promoter lead to the potential application of heterologous protein production in methylotrophic yeast. In this review, we describe the recent progress of heterologous protein production technology in methylotrophic yeast and introduce Ogataea minuta as an alternative production host as a substitute for K. phaffii and O. polymorpha.

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“…Pretreatment of substrates before fermentation is a common method to help the bacteria to assimilate the substrate and to produce the biopolymer. The pretreatment are the pretreatment of kitchen waste [32], the pretreatment of agro-industrial by-products [33], the pretreatment of lignocellulosic substrates [34] and also molasse [18]. Prior to microbial cultivation, the composition of various sugars and mineral was analyzed before and after pretreatment (Table 1).…”

Section: Pretreatment Of Molassementioning

confidence: 99%

Valorization of Sugar Industrial Waste (Molasse) via Polyhydroxyalkanoate Production for a Sustainable Circular Economy

MOUHO,

Yan,

Konan

et al. 2024

Preprint

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Sugar industries are among the oldest food processing factories. Regrettably, main waste from sugar industries, are discharged without treatment in the environment. There are many wastes from raw to final product in sugar processes. Industrial waste requires proper management; otherwise, it causes a major eﬀect on the environment. Molasse is one of the wastes from the sugar industry. However, it also has the potential to be transformed into polyhydroxyalkanoate (PHA) via fermentation. This approach could reduce waste and solve an environmental pollution. In this study, a fermentation was conducted in shake-flask with Cupriavidus Necator. Three types of pretreatments of molasse were conducted to increase PHA production: i) sulfuric acid pretreatment; ii) enzymatic pretreatment and iii) pretreatment with activated carbon. Molasse pretreated with enzyme, incubated in a water bath for 5 h (60 °C, 150 rpm), allows to accumulate up to a maximum of 8.20 g/L cell dry mass (CDW), with a PHA content of 71.79 wt%. The use of molasse for PHA production, has the potential to drive sugar industry toward a circular bioeconomy. Because PHB obtained will use to produce bioplastic bag, for containing sugar.

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Utilization potential of agro-industrial by-products and waste sources: Laccase production in bioreactor with Pichia pastoris

Cited by 7 publications

References 45 publications

Valorization of Food Waste into Single-Cell Protein: An Innovative Technological Strategy for Sustainable Protein Production

Valorization of Food Waste into Single-Cell Protein: An Innovative Technological Strategy for Sustainable Protein Production

Recent progress on heterologous protein production in methylotrophic yeast systems

Valorization of Sugar Industrial Waste (Molasse) via Polyhydroxyalkanoate Production for a Sustainable Circular Economy

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