Utilization of sugarcane straw for production of β-glucan biopolymer by Lasiodiplodia theobromae CCT 3966 in batch fermentation process

Abdeshahian, Peyman; Ascencio, Jesús J.; Philippini, Rafael R.; Antunes, Felipe Antônio Fernandes; Santos, Júlio César dos; Silva, Sílvio Silvério da

doi:10.1016/j.biortech.2020.123716

Cited by 22 publications

(6 citation statements)

References 42 publications

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“…Lasiodiplodan samples revealed a similar chemical structure, as shown in Figure 7A. The broad bands indicated a deep intensity in the 3274 cm −1 regions attributed to an R-OH stretching vibration [15]. In the spectral regions, close to 2900 and 1400 cm −1 , the deformation vibrations are typical on lasiodiplodan [14,37].…”

Section: Ftir Dsc and Xrd Analysismentioning

confidence: 74%

“…Then, 1650 cm −1 bands correspond to a glucose ring, while symmetrical vibrations of C-O-C characteristic bonds on carbohydrates typically have absorption in 1075 cm −1 [16]. Finally, the 890 cm −1 bands with low intensity are typical of glycosidic bonds, attributed to the β-configuration of lasiodiplodan have been described in studies on lasiodiplodan production [14][15][16]38]. Figure 7B shows three heat stages on lasiodiplodan samples.…”

Section: Ftir Dsc and Xrd Analysismentioning

confidence: 76%

“…Biotechnological lasiodiplodan production can cater to its growing demand in society. Nonetheless, the low production levels of lasiodiplodan through a microbial route is one of the bottlenecks for its commercial manufacturing [9,10,[14][15][16]. Sugarcane bagasse (SCB) is a low-cost renewable carbon source primarily used for energy and steam generation in sugar mills and distillery [17].…”

Section: Introductionmentioning

confidence: 99%

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Comparative Highly Efficient Production of β-glucan by Lasiodiplodia theobromae CCT 3966 and Its Multiscale Characterization

et al. 2021

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Lasiodiplodan, a (1→6)-β-d-glucan, is an exopolysaccharide with high commercial value and many applications in food, pharmaceuticals, and cosmetics. Current industrial production of β-glucans from crops is mostly by chemical routes generating hazardous and toxic waste. Therefore, alternative sustainable and eco-friendly pathways are highly desirable. Here, we have studied the lasiodiplodan production from sugarcane bagasse (SCB), a major lignocellulosic agricultural residue, by Lasiodiplodia theobromae CCT 3966. Lasiodiplodan accumulated on SCB hydrolysate (carbon source) supplemented with soybean bran or rice bran (nitrogen source) was 16.2 [6.8 × 103 Da] and 22.0 [7.6 × 103 Da] g/L, respectively. Lasiodiplodan showed high purity, low solubility, pseudoplastic behavior and was composed of glucose units. Moreover, the exopolysaccharides were substantially amorphous with moderate thermal stability and similar degradation temperatures. To our knowledge, this is the first report on the highest production of SCB-based lasiodiplodan to date. L. theobromae, as a microbial cell factory, demonstrated the commercial potential for the sustainable production of lasiodiplodan from renewable biomass feedstock.

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Section: Ftir Dsc and Xrd Analysismentioning

confidence: 74%

Section: Ftir Dsc and Xrd Analysismentioning

confidence: 76%

Section: Introductionmentioning

confidence: 99%

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Comparative Highly Efficient Production of β-glucan by Lasiodiplodia theobromae CCT 3966 and Its Multiscale Characterization

et al. 2021

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“…The XG5 EPS decrease may be due to the use of XG5 EPS by L. pseudomesenteroides XG5 after 24 h of fermentation. Similarly, Abdeshahian et al (2020) found that β-glucan accumulated in the ini- tial 72 h of fermentation time and later consumed. It is remarkable that the higher dry cell weight was achieved in 24 h of batch fermentation, and the maximum yield of XG5 EPS was harvested at this time.…”

Section: Fed-batch Fermentation Of Xg5 Eps In 50 L Bioreactormentioning

confidence: 78%

Pilot-scale production of exopolysaccharide from Leuconostoc pseudomesenteroides XG5 and its application in set yogurt

Pan

Wang

Qu³

et al. 2022

Journal of Dairy Science

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Exopolysaccharide from Leuconostoc pseudomesenteroides XG5 (XG5 EPS) is a linear dextran that is built by glucose units via α-1,6 glycosidic bond. The primary objective of this study was to investigate the yield of XG5 EPS and its application in set yogurt. In laboratory scale, the culture conditions of XG5 EPS production were optimized using the L9 (3 3 ) orthogonal test. Here, the optimized yield of XG5 EPS was 26.02 g/L under the conditions of 100 g/L sucrose, initial pH 7.0, 25°C incubation, and 100 rpm for 36 h in a shaking flask. Based on the optimized parameters of laboratory scale, a pilot fed-batch fermentation was performed in a 50-L bioreactor with an adjusted agitation speed of 20 rpm. The XG5 EPS yield reached 40.07 g/L in fedbatch fermentation, which was 54% higher than that achieved in laboratory scale. In addition, XG5 EPS was added into set yogurt to investigate its effect on the stability of set yogurt. Our data demonstrated that the XG5 EPS improved the water-holding capacity, texture profile, and viscosity of set yogurt during cold storage compared with the controls. In particular, addition of 0.5% XG5 EPS increased the structure of 3-dimensional network of set yogurt, which eventually improved the physical stability of the set yogurt. Overall, this study provided new insights for exploring the pilot scale production and application of dextran.

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“…The study fulfilled by Abdeshahian et al [36] showed that an untreated sample of sugarcane straw was composed of 41.25% cellulose, 35.17% hemicellulose, 17.25% lignin, and 2.81% ash. This indicates that sugarcane straw could be regarded as low-cost lignocellulosic biomass to obtain glucose, which can be further used for the production of glucan biopolymer through microbial fermentation process.…”

Section: Introductionmentioning

confidence: 99%

Fermentative Production of Lasiodiplodan by Lasiodiplodia theobromae CCT3966 from Pretreated Sugarcane Straw

et al. 2021

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Lasiodiplodan is a β-glucan polymer with different interesting characteristics, including therapeutic properties. It is an extracellular product, which is produced by the filamentous fungus Lasiodiplodia theobromae, using glucose as a substrate. In the present work, the production of lasiodiplodan was studied by the utilization of sugarcane straw as a low-cost carbon source. Glucose-rich sugarcane straw hydrolysate was obtained by a sequential pretreatment with dilute nitric acid (1% v/v) and sodium hydroxide (1% w/v), followed by enzymatic hydrolysis. The fermentation process was conducted by the cultivation of the strain Lasiodiplodia theobromae CCT3966 in sugarcane straw hydrolysate in a shake flask at 28 °C for 114 h. It was found that hydrolysate obtained after enzymatic hydrolysis contained 47.10 gL−1 of glucose. Fermentation experiments of lasiodiplodan synthesis showed that the peak yield and productivity of 0.054 gg−1 glucose consumed and 0.016 gL−1 h−1, respectively, were obtained at 72 h fermentation time. Fungal growth, glucose consumption, and lasiodiplodan production from sugarcane straw hydrolysate presented a similar pattern to kinetic models. The study on the chemical structure of lasiodiplodan produced showed it had a β-glucan construction. The current study revealed that sugarcane straw is a promising substrate for the production of lasiodiplodan.

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Utilization of sugarcane straw for production of β-glucan biopolymer by Lasiodiplodia theobromae CCT 3966 in batch fermentation process

Cited by 22 publications

References 42 publications

Comparative Highly Efficient Production of β-glucan by Lasiodiplodia theobromae CCT 3966 and Its Multiscale Characterization

Comparative Highly Efficient Production of β-glucan by Lasiodiplodia theobromae CCT 3966 and Its Multiscale Characterization

Pilot-scale production of exopolysaccharide from Leuconostoc pseudomesenteroides XG5 and its application in set yogurt

Fermentative Production of Lasiodiplodan by Lasiodiplodia theobromae CCT3966 from Pretreated Sugarcane Straw

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