Fermentative Production of Lasiodiplodan by Lasiodiplodia theobromae CCT3966 from Pretreated Sugarcane Straw

Abstract

Lasiodiplodan is a β-glucan polymer with different interesting characteristics, including therapeutic properties. It is an extracellular product, which is produced by the filamentous fungus Lasiodiplodia theobromae, using glucose as a substrate. In the present work, the production of lasiodiplodan was studied by the utilization of sugarcane straw as a low-cost carbon source. Glucose-rich sugarcane straw hydrolysate was obtained by a sequential pretreatment with dilute nitric acid (1% v/v) and sodium hydroxide (1% w/v), followed by enzymatic hydrolysis. The fermentation process was conducted by the cultivation of the strain Lasiodiplodia theobromae CCT3966 in sugarcane straw hydrolysate in a shake flask at 28 °C for 114 h. It was found that hydrolysate obtained after enzymatic hydrolysis contained 47.10 gL−1 of glucose. Fermentation experiments of lasiodiplodan synthesis showed that the peak yield and productivity of 0.054 gg−1 glucose consumed and 0.016 gL−1 h−1, respectively, were obtained at 72 h fermentation time. Fungal growth, glucose consumption, and lasiodiplodan production from sugarcane straw hydrolysate presented a similar pattern to kinetic models. The study on the chemical structure of lasiodiplodan produced showed it had a β-glucan construction. The current study revealed that sugarcane straw is a promising substrate for the production of lasiodiplodan.

Exopolysaccharides of Fungal Origin: Properties and Pharmaceutical Applications

“…The use of soybean molasses, sugarcane straw, or sugarcane bagasse as a low-cost carbon source, rice bran extract, and soybean bran extract as a nitrogen source, respectively, has been reported as promising substrates for lasiodiplodan production through submerged fermentation [117][118][119]. Furthermore, dextrose, glucose, mannitol, sucrose, fructose, maltose, and lactose have been tested as carbon sources, followed by potassium nitrate, ammonium sulfate, and ammonium chloride as nitrogen sources [120].…”

Some new insights into the biological activities of carboxymethylated polysaccharides from Lasiodiplodia theobromae

“…Besides its pathogenicity, L. theobromae MMPI has been catching the interest of researchers because of the biosynthesis of Lasiodiplodan, an β-glucan [ 1 – 3 ] exopolysaccharide. Exopolysaccharides (EPS) can be found attached to the cell surface or in the fungal extracellular medium as slime.…”

“…LaEPS has been reported as having an antiproliferative effect on breast cancer cells, hypoglycemic function, antimicrobial and antioxidant activities, making it an attractive biopolymer for pharmaceutical, food, and cosmetic applications [ 3 , 17 , 20 , 21 ]. Moreover, Lasiodiplodan has an easier production compared to the other exopolysaccharides since its fermentation is submerged, and its recovery is made by precipitation methods from the cell-free fermentation broth in ethanol.…”