Utilization of spent coffee grounds for isolation and stabilization of Paenibacillus chitinolyticus CKS1 cellulase by immobilization

Abstract

This study has explored the feasibility of using spent coffee grounds as a good supporting material for the Paenibacillus chitinolyticus CKS1 cellulase immobilization. An optimal operational conditions in a batch-adsorption system were found to be: carrier mass of 12 g/L, under the temperature of 45 °C and no pH adjustments. The immobilization yield reached about 71%. An equilibrium establishment between the cellulase and the carrier surface occurred within 45 min, whereas the process kinetics may be predicted by the pseudo-second-order model. An immobilized cellulase preparation expressed very good avicelase activity, this reached up to 2.67 U/g, and revealed an improved storage stability property, compared to free enzyme sample counterpart. The addition of metal ions, such as K+ and Mg2+ did not affect positively immobilization yield results, but on the contrary, contributed to an improved bio-activities of the immobilized cellulase, thus may be employed before each enzyme application. The method developed in this study offers a cheap and effective alternative for immediate enzyme isolation from the production medium and its stabilization, compared to other carriers used for the immobilization.

Secado de Pulpa de Café: Condiciones de Proceso, Modelación Matemática y Efecto sobre Propiedades Fisicoquímicas

“…Actualmente para uso de estos residuos se elabora ensilaje para la alimentación animal, torta de pulpa de café, jugo tratado con microorganismos para el consumo animal (Noriega et al, 2009), adicionalmente han sido utilizados para cultivo de hongos (Murthy y Manonmani, 2008), producción de enzimas (Buntić et al, 2016;Ngo y Phan, 2016;Torres-Mancera et al, 2011), biocombustibles (Gouvea et al, 2009;Woldesenbet, Woldeyes, y Chandravanshi, 2016) y compostaje (Hachicha et al, 2012). Teniendo en cuenta que la pulpa de café es altamente perecedera debido a su alto contenido de humedad, se generan pérdidas por deterioro químico y microbiano (Ruiz-López et al, 2011).…”

Waste Management in the Agri-Food Industry: The Conversion of Eggshells, Spent Coffee Grounds, and Brown Onion Skins into Carriers for Lipase Immobilization

“…However, this raises the question of the cost-effectiveness of the manufacturing process of the biosensor on a support that is the inner membrane of the brown onion, since the brown onion membrane is an edible part of the onion and not a waste product in the processing of the brown onion. The same can be observed in the case of spent coffee grounds as a potential carrier for enzyme immobilization, for which there are only four literature data points [125][126][127][128]. Chen et al [125] immobilized −glucosidase by covalent binding to spent coffee grounds activated with glutaraldehyde and used it to convert isoflavone glycosides to their aglycones from black soy milk.…”

“…The immobilized −glucosidase was stable for 20 days without losing activity in 30 batches. In their work, Buntić et al [126][127][128] performed immobilization of cellulase by adsorption and covalent binding. The results indicate an improvement in storage stability and activity of immobilized cellulase compared to its free form.…”

Cellulases: From Lignocellulosic Biomass to Improved Production

“…Cross-linking, copolymerization, fiber ultra-filtration, aqueous two-phase systems, and the usage of a wide range of both natural and synthetic supports can all be used to efficiently immobilize cellulase [5,87,91,92]. Even on an industrial scale, the cross-linked cellulase aggregates constitute a useful form of immobilized enzyme for usage in largescale production procedures and biotransformation [93].…”