Utilization of phytate by some yeasts

Lambrechts, Christel; Boze, Hélène; Moulin, G.; Galzy, P.

doi:10.1007/bf01030915

Cited by 82 publications

(47 citation statements)

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“…Although phytases are produced by various groups of microbes (Lim et al 2007), yeasts, which are simple mostly non-pathogenic Eukarya, with high extracellular phytase activity (Lambrechts et al 1992), serve as ideal candidates to study the potential applications of in situ calcium-phosphate precipitation.…”

Section: Resultsmentioning

confidence: 99%

“…Arxula adeninivorans CBS 7350 was obtained from the CBS (Utrecht, The Netherlands) and was grown at room temperature in (1) liquid minimal medium, (2) on solid medium plates prepared according to Lambrechts et al (1992), and (3) in CaPh amended minimal medium in batch culture; samples for A 600 were taken at various time points over 26 h. To solid medium, 1 % Ca 2+ -phytate (myo-inositol hexakisphosphate, calcium salt; Sigma-Aldrich, St. Louis, MO) was added as a sole C and P source, pH being adjusted to 6.5. Agar plates contained 2 % Bactoagar (Difco, USA).…”

Section: Methodsmentioning

confidence: 99%

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Microbial phytase-induced calcium-phosphate precipitation — a potential soil stabilization method

Roeselers

Loosdrecht

2010

Folia Microbiol

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ABSTRACT. Two hypotheses were tested: (1) microbial dephosphorylation of phytate in the presence of Ca 2+ ions will result in the precipitation of hydroxyapatite-like crystals and (2) precipitation of calcium-phosphate crystals on and between sand-like particles can cause cementation. A growing culture of the dimorphic phytase-active yeast Arxula adeninivorans was introduced into a column filled with quartz particles and subsequently a liquid growth medium amended with calcium phytate was pumped through the column resulting in increased strength and stiffness of the quartz particle matrix. Environmental scanning electron microscope analysis combined with energy-dispersive X-ray measurement revealed cementation of the quartz particles by calcium-phosphate crystals. This microbial mineralization process could provide a novel approach to improving the mechanical properties like strength and stiffness of sandy soils. Microbial metabolic activities often contribute to selective cementation processes by producing relatively insoluble organic and inorganic compounds. Microbially induced biomineralization, such as calcite (CaCO 3 ) and calcium hydroxyapatite [Ca 5 (PO 4 ) 3 OH] precipitation, has been described in a wide variety of environments (Boquet et al. 1973;Schmittner and Giresse 1999;Belzer et al. 2006;Canaveras et al. 2006;Kremer et al. 2008). The formation of calcium hydroxyapatite has received considerable attention as a result of its importance in biological calcification. Calcium phosphates are the main constituent of teeth and bones and have been found in pathological mineral deposits in the form of urinary and kidney stones.The importance of microbially induced cementation has been widely recognized in petroleum, geological and civil engineering (Dejong et al. 2006;Ivanov and Chu 2008). There is a growing interest in methods to improve the mechanical properties of sandy soils using microbially induced biochemical reactions in the subsurface (Whiffin et al. 2007). These methods simulate natural diagenetic processes that transform loose sand to sandstone. It has been documented that cracks in rock formations, especially in oil reservoirs, could be remediated by microbial mineral precipitation (Jack 1991).Phytic acid salts or phytates (myo-inositol hexakisphosphates) are abundant plant constituents. They typically account for 60-90 % of total seed phosphorus. Phytate is a natural by-product of fermentation processes and a waste product in the fermentation industry.Phytases, a specific group of phosphatases, catalyze hydrolysis of the phosphate ester bonds of phytates (by the addition of water) (Cosgrove 1966;Mullaney et al. 2000). They have been found in various eukaryotic and prokaryotic microorganisms (Lassen et al. 2001;Lim et al. 2007). Extracellular phytases from several filamentous fungi and yeasts have been studied in detail. The dimorphic yeast A.a. can utilize phytate as a sole C and P source by secreting high level of phytase into its environment (Sano et al. 1999).This paper aims to explore the po...

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Microbial phytase-induced calcium-phosphate precipitation — a potential soil stabilization method

Roeselers

Loosdrecht

2010

Folia Microbiol

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“…Free inorganic phosphate was assayed in the culture supernatant using the ammonium molybdate method (Heinonen and Lahti, 1981) modified by Lambrechts et al (1992). Phosphate concentrations from 0. l0 to 5 rnM were measured.…”

Section: Methodsmentioning

confidence: 99%

“…The culture supernatant phytic phosphate content was assayed by determination of the phosphate concentration released after hydrolysis of phytate by the phytase of Aspergillusflcuum (Sigma) as described by Lambrechts et al (1992). A calibration curve was plotted for sodium phytate (Sigma) concentrations from 0.01 to 0.5 rnM.…”

Section: Methodsmentioning

confidence: 99%

Influence of culture conditions on the biosynthesis of Schwanniomyces castellii phytase

Lambrechts¹,

Boze²,

Segueilha³

et al. 1993

Biotechnol Lett

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“…Initially, the cultures were isolated from Musa acuminata fruit surface (B4), Malus domestica fruit surface (PA4) and Sorghum bicolor stem juice (SS1) from Pantnagar (29.00°N/79.28°E), a subtropical region of Indian Shiwalik Himalayas. The active cultures were screened qualitatively for phytase production using phytase screening medium (PSM) as described by Lambrechts et al (1992). Sodium phytate (2 gL -1 ) was filter sterilized and added to the sterilized medium before pouring.…”

Section: Yeast Cultures and Screening For Phytase Productionmentioning

confidence: 99%

In vitro degradation of natural animal feed substrates by intracellular phytase producing Shiwalik Himalayan budding yeasts

Ch¹,

Suyal²,

Tewari³

2013

Afr. J. Microbiol. Res.

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Himalayas are the natural reservoir of complex and diversified gene pool. Three Shiwalik Himalayan intracellular phytase producing budding yeasts were assayed for in vitro degradation of natural animal feed substrates. Phosphorus availability was found to enhance upto 70% yeast cultures during in vitro biodegradation of natural animal feed substrates. A direct correlation between intracellular phosphate concentration and phytase activity suggested the use of whole cell preparations in place of purified enzymes. Zymogram analysis revealed the presence of single high molecular weight isoform of the enzyme phytase. Based on 5.8S-ITS-rDNA sequencing, using ITS1 and ITS4 primers, the cultures were identified as Candida tropicalis (B4), Issatchenkia orientalis (PA4) and Pichia gluermondii (SS1). Indigenous I. orientalis strain PA4 was found superior among all the yeasts strains and therefore can be developed as successful inoculant for animal nutrition as well as environmental management under Himalayan ecosystems.

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Utilization of phytate by some yeasts

Cited by 82 publications

References 8 publications

Microbial phytase-induced calcium-phosphate precipitation — a potential soil stabilization method

Microbial phytase-induced calcium-phosphate precipitation — a potential soil stabilization method

Influence of culture conditions on the biosynthesis of Schwanniomyces castellii phytase

In vitro degradation of natural animal feed substrates by intracellular phytase producing Shiwalik Himalayan budding yeasts

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